1990
Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis
Hotez P, Haggerty J, Hawdon J, Milstone L, Gamble H, Schad G, Richards F. Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis. Infection And Immunity 1990, 58: 3883-3892. PMID: 2254016, PMCID: PMC313750, DOI: 10.1128/iai.58.12.3883-3892.1990.Peer-Reviewed Original ResearchConceptsProtease activitySodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisProtein substratesSulfate-polyacrylamide gel electrophoresisMr polypeptideNematode Strongyloides stercoralisBovine elastinPossible functionsBiochemical similaritiesAlkaline pH optimumLarvaeConnective tissue macromoleculesHuman hookworms Ancylostoma duodenaleInvasion propertiesGel electrophoresisEnzyme activityAncylostoma speciesBiochemical differencesA. caninum larvaeMinor bandsProtease compositionSmall peptidesHuman fibronectinMajor band
1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture