1992
Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen.
Smith L, Wertelecki W, Milstone L, Petty E, Seashore M, Braverman I, Jenkins T, Byers P. Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen. American Journal Of Human Genetics 1992, 51: 235-44. PMID: 1642226, PMCID: PMC1682688.Peer-Reviewed Original ResearchConceptsAmino-terminal propeptideFragile skinType IConnective tissue disordersEhlers-Danlos syndromeLax jointsType VIIAffected animalsSkinChildrenCultured fibroblastsNormal cellsEhlers-Danlos syndrome type VIIPro alphaDisordersPropeptideAlphaDermatosparaxisProteolytic processingSepsisN-proteinaseCellsSyndromeCollagen fibrilsMethotrexate induces differentiation of human keratinocytes.
Schwartz P, Barnett S, Atillasoy E, Milstone L. Methotrexate induces differentiation of human keratinocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 594-598. PMID: 1731329, PMCID: PMC48285, DOI: 10.1073/pnas.89.2.594.Peer-Reviewed Original ResearchConceptsEffect of methotrexateAbility of methotrexateProliferation of keratinocytesProportion of cellsMarkers of differentiationHuman epidermal keratinocytesMethotrexateTherapeutic actionEpidermal keratinocytesMarked increaseHuman keratinocytesKeratinocytesEnvelope proteinSDS/PAGE profilesInvolucrin synthesisNormal cellsTerminal differentiationTissue homeostasisThymine deoxyribonucleotidesDifferentiationCellsPsoriasisCell morphologyReproducible changesDose
1991
Antiviral Activity of 5-Iodo-2’-Deoxyuridine and Related Drugs in Human Keratinocytes Infected in vitro with Herpes simplex Virus Type 1
Reuveni H, Bull CO, Landry ML, Milstone LM, Schwartz PM. Antiviral Activity of 5-Iodo-2’-Deoxyuridine and Related Drugs in Human Keratinocytes Infected in vitro with Herpes simplex Virus Type 1. Skin Pharmacology And Physiology 1991, 4: 291-297. PMID: 1665069, DOI: 10.1159/000210964.Peer-Reviewed Original ResearchConceptsGuinea pig embryo cellsHerpes simplex virus type 1Simplex virus type 1Virus type 1Antiviral activityThymidine phosphorylaseHuman keratinocytesType 1Cutaneous viral infectionsTopical antiviral agentsAntiviral agentsViral infectionExperimental animalsEmbryo cellsRelated drugsIUdRInfected cellsKeratinocytesDrugsCellsActivityInfectionPhosphorylase
1982
Retinoic Acid Causes Premature Desquamation of Cells from Confluent Cultures of Stratified Squamous Epithelia
Milstone L, McGuire J, Lavigne J. Retinoic Acid Causes Premature Desquamation of Cells from Confluent Cultures of Stratified Squamous Epithelia. Journal Of Investigative Dermatology 1982, 79: 253-260. PMID: 7130743, DOI: 10.1111/1523-1747.ep12500073.Peer-Reviewed Original Research
1981
Different polypeptides form the intermediate filaments in bovine hoof and esophageal epithelium and in aortic endothelium.
Milstone L, McGuire J. Different polypeptides form the intermediate filaments in bovine hoof and esophageal epithelium and in aortic endothelium. Journal Of Cell Biology 1981, 88: 312-316. PMID: 7193680, PMCID: PMC2111748, DOI: 10.1083/jcb.88.2.312.Peer-Reviewed Original ResearchConceptsAmino acid sequence homologyFilament-forming proteinsPeptide mapsLimited proteolytic digestionSDS-polyacrylamide gelsDifferent bovine tissuesSequence homologyDifferent polypeptidesEndothelial cellsCultured endothelial cellsPolypeptideIntermediate filamentsProteolytic digestionPolyacrylamide gelsBovine tissuesFilamentsCellsFragmentsHomologyEpitheliumProteinBovine hoofAortic endotheliumEsophageal epitheliumViable portion
1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture
1976
δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
Milstone L, Zelenka P, Piatigorsky J. δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells. Developmental Biology 1976, 48: 197-204. PMID: 1254080, DOI: 10.1016/0012-1606(76)90084-1.Peer-Reviewed Original ResearchConceptsΔ-crystallin synthesisChick lens epithelial cellsChick lens cellsEmbryonic lens epitheliumLens fiber cellsLens epithelial cellsFiber cellsLens cellsDifferential stimulationProtein synthesisCultured cellsMRNALens epitheliumEpithelial cellsTissue cultureCellsDifferentiationOverall stimulationAccumulationStimulationSpecializationCultureSynthesisOvo
1975
Rates of protein synthesis in explanted embryonic chick lens epithelia: Differential stimulation of δ-crystallin synthesis
Milstone L, Piatigorsky J. Rates of protein synthesis in explanted embryonic chick lens epithelia: Differential stimulation of δ-crystallin synthesis. Developmental Biology 1975, 43: 91-100. PMID: 1149925, DOI: 10.1016/0012-1606(75)90133-5.Peer-Reviewed Original ResearchConceptsChick lens epitheliumΔ-crystallin synthesisProtein synthesisΔ-crystallinDifferential protein synthesisLens fiber cellsLens fiber differentiationLens epitheliumProtein degradationFiber cellsFiber differentiationBulk proteinTotal protein synthesisRate of synthesisIntracellular valineLabeling periodNumber of cellsProteinFetal calf serumCellsDifferential stimulationBiochemical changesCalf serumTotal proteinEpithelium
1973
Differentiation of lens fibers in explanted embryonic chick lens epithelia
Piatigorsky J, Rothschild S, Milstone L. Differentiation of lens fibers in explanted embryonic chick lens epithelia. Developmental Biology 1973, 34: 334-345. PMID: 4596973, DOI: 10.1016/0012-1606(73)90362-x.Peer-Reviewed Original ResearchConceptsChick lens epitheliumLens epitheliumLens fibersLens epithelial cellsRibosomal clustersRibosomal RNADelta-crystallinTissue cultureSingle-strand breaksFiber differentiationCell membraneLong cellsMean cell lengthCell lengthTotal RNACellular outgrowthDifferentiationEpithelial cellsSurface membraneRNAOrganelle densityChick embryosGranular matrixBiochemical changesCells