2008
PKC-Induced Intracellular Trafficking of CaV2 Precedes Its Rapid Recruitment to the Plasma Membrane
Zhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA. PKC-Induced Intracellular Trafficking of CaV2 Precedes Its Rapid Recruitment to the Plasma Membrane. Journal Of Neuroscience 2008, 28: 2601-2612. PMID: 18322103, PMCID: PMC2830008, DOI: 10.1523/jneurosci.4314-07.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCPlasma membraneGrowth conesLatrunculin BIntracellular traffickingActin polymerizationIntact microtubulesIntact actinKinase CChannel insertionPKC activationIon channelsMicrotubule polymerizationRapid recruitmentOrganellesLamellipodiumSubunitsMicrotubulesActinMembraneActivationRecruitmentCone terminalsNew sites
2006
Pharmacological activation and inhibition of Slack (Slo2.2) channels
Yang B, Gribkoff VK, Pan J, Damagnez V, Dworetzky SI, Boissard CG, Bhattacharjee A, Yan Y, Sigworth FJ, Kaczmarek LK. Pharmacological activation and inhibition of Slack (Slo2.2) channels. Neuropharmacology 2006, 51: 896-906. PMID: 16876206, DOI: 10.1016/j.neuropharm.2006.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Infective Agents, LocalBepridilBithionolCalcium Channel BlockersCell Line, TransformedDose-Response Relationship, DrugDose-Response Relationship, RadiationElectric StimulationEnzyme ActivationEnzyme InhibitorsHumansMembrane PotentialsOocytesPatch-Clamp TechniquesPotassium Channels, Calcium-ActivatedQuinidineTransfectionXenopusConceptsSlack channelsConcentration-dependent mannerIschemic injuryPharmacological activationKNa channelsMammalian brainFiring ratePharmacological propertiesChannel subunitsReversible increaseChannel activityCell linesBepridilHEK cellsRobust activatorNeuronsStable cell linesInhibitionExcised patchesXenopus oocytesPresent studyBithionolChannel openingSpecific roleMembrane patches
1996
Regulation of potassium channels by protein kinases
Jonas E, Kaczmarek L. Regulation of potassium channels by protein kinases. Current Opinion In Neurobiology 1996, 6: 318-323. PMID: 8794088, DOI: 10.1016/s0959-4388(96)80114-0.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAnimalsCell MembraneEnzyme ActivationHumansKineticsPhosphorylationPotassium ChannelsProtein KinasesSerineThreonineTyrosine
1992
Recruitment of Ca2+ channels by protein kinase C during rapid formation of putative neuropeptide release sites in isolated Aplysia neurons
Knox R, Quattrocki E, Connor J, Kaczmarek L. Recruitment of Ca2+ channels by protein kinase C during rapid formation of putative neuropeptide release sites in isolated Aplysia neurons. Neuron 1992, 8: 883-889. PMID: 1316764, DOI: 10.1016/0896-6273(92)90202-o.Peer-Reviewed Original Research
1991
Phosphorylation of membrane‐associated proteins by phorbol esters in isolated bag cell neurons of Aplysia
Azhderian E, Kaczmarek L. Phosphorylation of membrane‐associated proteins by phorbol esters in isolated bag cell neurons of Aplysia. Developmental Neurobiology 1991, 22: 105-115. PMID: 2030336, DOI: 10.1002/neu.480220202.Peer-Reviewed Original ResearchConceptsProtein kinase CBag cell neuronsKinase CPlasma membrane-containing fractionsProtein kinase C inhibitor H7Membrane-associated proteinsPhorbol esterExtent of phosphorylationMembrane-containing fractionsCell neuronsMembrane proteinsProtein phosphorylationNew speciesPhosphorylation statePlasma membraneTetradecanoyl phorbol 13Inhibitor H7Inactive phorbol esterIntact cellsPhosphate incorporationProtein extractsPhosphoproteinExtracellular mediumPhosphorylationProtein
1987
Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons
Strong J, Fox A, Tsien R, Kaczmarek L. Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons. Nature 1987, 325: 714-717. PMID: 2434853, DOI: 10.1038/325714a0.Peer-Reviewed Original ResearchConceptsProtein kinase CBag cell neuronsKinase CProtein kinaseCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseAplysia bag cell neuronsCell neuronsCalcium channelsSingle-channel levelExcitable cellsKinaseUntreated cellsUnitary conductanceCalcium currentPeptidergic bag cell neuronsVoltage-activated calcium channelsCardiac muscleDifferent unitary conductancesCellsDifferent mechanismsVoltage-activated calcium currentsChannel levelSecond classNeurons
1986
Phorbol Esters, Protein Phosphorylation and the Regulation of Neuronal Ion Channels
Kaczmarek L. Phorbol Esters, Protein Phosphorylation and the Regulation of Neuronal Ion Channels. Journal Of Experimental Biology 1986, 124: 375-392. PMID: 2428907, DOI: 10.1242/jeb.124.1.375.Peer-Reviewed Original ResearchConceptsProtein kinase CKinase CProtein kinase C resultsPhorbol esterNeuronal ion channelsProtein phosphorylationPlasma membraneLipid environmentIntact cellsIon channelsSuch activatorsEndogenous diacylglycerolVertebratesDiacylglycerolSynthetic diacylglycerolPhosphatidyl serineActivatorEndogenous electrical propertiesInactive speciesSynaptic transmissionInvertebratesCalcium channelsKinasePhosphorylationNervous system
1985
Enhancement of calcium current in Aplysia neurones by phorbol ester and protein kinase C
DeRiemer SA, Strong JA, Albert KA, Greengard P, Kaczmarek LK. Enhancement of calcium current in Aplysia neurones by phorbol ester and protein kinase C. Nature 1985, 313: 313-316. PMID: 2578617, DOI: 10.1038/313313a0.Peer-Reviewed Original ResearchConceptsProtein kinase CKinase CProtein kinase presentEndogenous protein kinase CKinase presentProtein kinase1Molecular mechanismsCellular componentsPhorbol ester TPAIon channelsPhorbol esterMammalian brainTumor-promoting phorbol ester TPAMollusc AplysiaPhysiological propertiesEnzymeNeuronal excitabilityDirect evidenceKinase1PhosphorylationProteinHigh concentrationsActivationAplysia