Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing
Zhang H, Pan H, Zhou C, Wei Y, Ying W, Li S, Wang G, Li C, Ren Y, Li G, Ding X, Sun Y, Li G, Song L, Li Y, Yang H, Liu Z. Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Development 2018, 145: dev168906. PMID: 30275281, DOI: 10.1242/dev.168906.Peer-Reviewed Original ResearchConceptsGene functionMultiple genesHair cell generationProtein-coding sequencesMulti-gene interactionsModel organismsFunctional genesZygotic mutationsGene crucialHigh-throughput screeningBase editingMouse mutantsStop codonSingle mutationGenesCRISPRCell generationGenetic mutationsMutationsMouse breedingNormal hearing functionDrosophilaMutantsBreedingCodon