2008
Diffusible retinal secretions regulate the expression of tight junctions and other diverse functions of the retinal pigment epithelium.
Sun R, Peng S, Chen X, Zhang H, Rizzolo LJ. Diffusible retinal secretions regulate the expression of tight junctions and other diverse functions of the retinal pigment epithelium. Molecular Vision 2008, 14: 2237-62. PMID: 19057659, PMCID: PMC2593753.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdherens JunctionsAnimalsCell MembraneCells, CulturedChick EmbryoCluster AnalysisCulture Media, ConditionedDiffusionElectric ImpedanceExtracellular MatrixGene Expression ProfilingGene Expression Regulation, DevelopmentalGenomeIon ChannelsKineticsMelaninsMembrane ProteinsMicrotubulesOligonucleotide Array Sequence AnalysisPhagocytosisRetinal Pigment EpitheliumRNA, MessengerSubcellular FractionsTight JunctionsVisual PathwaysConceptsGene expression
2007
Analysis of the RPE transcriptome reveals dynamic changes during the development of the outer blood-retinal barrier.
Rizzolo LJ, Chen X, Weitzman M, Sun R, Zhang H. Analysis of the RPE transcriptome reveals dynamic changes during the development of the outer blood-retinal barrier. Molecular Vision 2007, 13: 1259-73. PMID: 17679949.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBlood-Retinal BarrierCell Cycle ProteinsChick EmbryoCluster AnalysisCollagenExtracellular MatrixGene Expression ProfilingIntercellular JunctionsLamininMembrane ProteinsMicrotubule-Associated ProteinsMyosinsOligonucleotide Array Sequence AnalysisPhagocytosisPigment Epithelium of EyeProtein SubunitsProteinsReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTranscription, GeneticConceptsPattern of expressionExtensive remodelingAffymetrix microarray chipsCell-cell junctionsSignal transduction pathwaysExtracellular matrix interactionsAdherens junction proteinsTranscellular ion transportCell surface receptorsGenomic approachesEntire genomeGene OntologyTransduction pathwaysSTEM softwareZO-3Biological pathwaysFunctional clusteringMatrix interactionsExtracellular matrixSurface receptorsJunctional complexesMicroarray chipContinuous remodelingFalse discovery rateAffymetrix software
2006
Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions
Luo Y, Fukuhara M, Weitzman M, Rizzolo LJ. Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions. Brain Research 2006, 1110: 55-63. PMID: 16859655, DOI: 10.1016/j.brainres.2006.06.059.Peer-Reviewed Original Research
2004
The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development
Rahner C, Fukuhara M, Peng S, Kojima S, Rizzolo LJ. The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development. Journal Of Cell Science 2004, 117: 3307-3318. PMID: 15226402, DOI: 10.1242/jcs.01181.Peer-Reviewed Original ResearchAnimalsCell SeparationCells, CulturedChick EmbryoCulture Media, ConditionedDNA PrimersElectric ImpedanceElectrophysiologyFibroblastsFreeze FracturingGene Expression Regulation, DevelopmentalMicroscopy, FluorescenceNerve Tissue ProteinsPigment Epithelium of EyePolymerase Chain ReactionRetinaReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight JunctionsTime Factors
2003
Apical and Basal Regulation of the Permeability of the Retinal Pigment Epithelium
Peng S, Rahner C, Rizzolo LJ. Apical and Basal Regulation of the Permeability of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2003, 44: 808-817. PMID: 12556417, DOI: 10.1167/iovs.02-0473.Peer-Reviewed Original Research
2000
Differential regulation of tight junction permeability during development of the retinal pigment epithelium
Ban Y, Rizzolo L. Differential regulation of tight junction permeability during development of the retinal pigment epithelium. American Journal Of Physiology - Cell Physiology 2000, 279: c744-c750. PMID: 10942725, DOI: 10.1152/ajpcell.2000.279.3.c744.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumPigment epitheliumBlood-brain barrierEmbryonic ageTight junctionsTight junction permeabilityLow calcium mediumRPE developmentAcetylneuraminic acidMannitol permeabilityJunction permeabilityEpithelial regionsCulture modelDifferent subpopulationsParacellular diffusionEpitheliumDifferential regulationAgeOrder of permeationRegulation of glucose transporters during development of the retinal pigment epithelium
Ban Y, Rizzolo L. Regulation of glucose transporters during development of the retinal pigment epithelium. Brain Research 2000, 121: 89-95. PMID: 10837896, DOI: 10.1016/s0165-3806(00)00028-6.Peer-Reviewed Original ResearchAnimalsBlood-Brain BarrierBlotting, NorthernChick EmbryoGene Expression Regulation, DevelopmentalGlucose Transporter Type 1Glucose Transporter Type 2Glucose Transporter Type 3Monosaccharide Transport ProteinsNerve Tissue ProteinsPigment Epithelium of EyeReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight JunctionsTwo secreted retinal factors regulate different stages of development of the outer blood–retinal barrier
Ban Y, Wilt S, Rizzolo L. Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier. Brain Research 2000, 119: 259-267. PMID: 10675776, DOI: 10.1016/s0165-3806(99)00183-2.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic AgonistsAnimalsBiological TransportBlood-Brain BarrierCatecholaminesCell FractionationCells, CulturedChick EmbryoChromatography, GelCulture Media, ConditionedDopamine AgonistsElectric ImpedanceEndopeptidasesEye ProteinsIndolesPigment Epithelium of EyeSerotonin Receptor AgonistsTight JunctionsConceptsRetinal pigment epitheliumBlood-retinal barrierTransepithelial electrical resistanceOuter blood-retinal barrierBlood-brain barrierEffects of astrocytesCultured retinal pigment epitheliumChick retinal pigment epitheliumPigment epitheliumNeural retinaRetinal factorsChick retinaRPE barrierRPE cellsRetinaEndothelial regionAstrocytesTight junctionsCulture modelDiffusible factorsDifferent stagesActive factorsJunction developmentMultistep processFactors
1998
Protein-Binding Domains of the Tight Junction Protein, ZO-2, Are Highly Conserved between Avian and Mammalian Species
Collins J, Rizzolo L. Protein-Binding Domains of the Tight Junction Protein, ZO-2, Are Highly Conserved between Avian and Mammalian Species. Biochemical And Biophysical Research Communications 1998, 252: 617-622. PMID: 9837755, DOI: 10.1006/bbrc.1998.9710.Peer-Reviewed Original ResearchConceptsProtein-binding domainsSrc homology 3ZO-2ZO-3Homologous domainsMAGUK protein familyDistinct PDZ domainsProtein binding domainsZO-1Apical junctional complexHomology 3PDZ domainProtein familySH3 domainAcidic domainEvolutionary pressureMammalian speciesJunctional complexesEmbryonic chicken retinaJunction proteinsTight junctionsDifferent functionsProteinTight junction proteinsSequence
1997
A culture model of development reveals multiple properties of RPE tight junctions.
Ban Y, Rizzolo L. A culture model of development reveals multiple properties of RPE tight junctions. Molecular Vision 1997, 3: 18. PMID: 9479009.Peer-Reviewed Original ResearchAnimalsCell CountCell Membrane PermeabilityChick EmbryoCulture Media, ConditionedCulture TechniquesFluorescent Antibody Technique, IndirectGene Expression Regulation, DevelopmentalHorseradish PeroxidaseInulinMannitolMembrane ProteinsPhalloidinePhosphoproteinsPigment Epithelium of EyeTight JunctionsTime FactorsZonula Occludens-1 ProteinRemodeling of junctional complexes during the development of the outer blood‐retinal barrier
Williams C, Rizzolo L. Remodeling of junctional complexes during the development of the outer blood‐retinal barrier. The Anatomical Record 1997, 249: 380-388. PMID: 9372172, DOI: 10.1002/(sici)1097-0185(199711)249:3<380::aid-ar9>3.0.co;2-y.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumApical junctional complexOuter blood-retinal barrierBlood-retinal barrierNeural retinaZO-1Junctional complexesOccludin expressionRPE developmentMature retinal pigment epitheliumRPE/choroidZO-1 expressionTight junction proteinsEmbryonic day 3Same molecular massEmbryonic day 10Adherens junctionsPigment epitheliumDay 3Day 10Homologous complexesMolecular massRetinaJunction proteinsJunction permeability
1995
The distribution of Na+,K+-atpase and 5a11 antigen in apical microvilli of the retinal pigment epithelium is unrelated to α-spectrin
Rizzolo L, Zhou S. The distribution of Na+,K+-atpase and 5a11 antigen in apical microvilli of the retinal pigment epithelium is unrelated to α-spectrin. Journal Of Cell Science 1995, 108: 3623-3633. PMID: 8586673, DOI: 10.1242/jcs.108.11.3623.Peer-Reviewed Original ResearchConceptsAlpha-spectrinPlasma membranePlasma membrane proteinsDevelopment of polarityLateral plasma membraneBasal plasma membraneMembrane proteinsCortical cytoskeletonMacromolecular complexesBeta spectrinAnkyrinChick embryogenesisApical poleCytoskeletonEmbryonic day 6RPE microvilliLateral membranesReceptor proteinMost epitheliaSpectrin distributionΑ-spectrinApical membraneIon pumpsApical microvilliProtein
1994
The neural retina maintains integrins in the apical membrane of the RPE early in development.
Rizzolo L, Zhou S, Li Z. The neural retina maintains integrins in the apical membrane of the RPE early in development. Investigative Ophthalmology & Visual Science 1994, 35: 2567-76. PMID: 8163344.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumNeural retinaAlpha 6Alpha 3Photoreceptor cell layerSubunit alpha 3Apical membraneBasolateral membraneBeta 1 subunitPigment epitheliumDistribution of integrinsBeta 1 familyRetinaBeta 1Interphotoreceptor matrixExplant culturesCell layerBasal membraneRetinal interfaceEpitheliumIndividual integrinsIntegrin distributionIntegrinsIntercellular interactionsChicken development
1993
Diffusible, retinal factors stimulate the barrier properties of junctional complexes in the retinal pigment epithelium
Rizzolo L, Li Z. Diffusible, retinal factors stimulate the barrier properties of junctional complexes in the retinal pigment epithelium. Journal Of Cell Science 1993, 106: 859-867. PMID: 8308068, DOI: 10.1242/jcs.106.3.859.Peer-Reviewed Original ResearchApical Orientation of the Microtubule Organizing Center and Associated γ-Tubulin during the Polarization of the Retinal Pigment Epithelium in Vivo
Rizzolo L, Joshi H. Apical Orientation of the Microtubule Organizing Center and Associated γ-Tubulin during the Polarization of the Retinal Pigment Epithelium in Vivo. Developmental Biology 1993, 157: 147-156. PMID: 8482407, DOI: 10.1006/dbio.1993.1119.Peer-Reviewed Original ResearchConceptsMicrotubule arraysCell polarityEmbryonic day 3Apical membraneGamma-tubulin fociJunctional complexesSimple epithelial cellsMicrotubule organizing centerDevelopment of epitheliaCentrosomal proteinsJuxtanuclear regionΓ-tubulinUnique reorganizationBasal axisGamma-tubulinCell-basement membrane interactionsFunctional polarityMicrotubule growthMembrane interactionsOrganizing centerPrimary cell culturesCultured cellsEpithelial cellsRetinal pigment epitheliumCell cultures
1991
Basement membrane stimulates the polarized distribution of integrins but not the Na,K-ATPase in the retinal pigment epithelium.
Rizzolo L. Basement membrane stimulates the polarized distribution of integrins but not the Na,K-ATPase in the retinal pigment epithelium. Molecular Biology Of The Cell 1991, 2: 939-949. PMID: 1667092, PMCID: PMC361892, DOI: 10.1091/mbc.2.11.939.Peer-Reviewed Original ResearchThe polarity of the retinal pigment epithelium is developmentally regulated
Rizzolo L, Heiges M. The polarity of the retinal pigment epithelium is developmentally regulated. Experimental Eye Research 1991, 53: 549-553. PMID: 1657628, DOI: 10.1016/0014-4835(91)90173-c.Peer-Reviewed Original Research
1990
The distribution of Na+,K+-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture
Rizzolo L. The distribution of Na+,K+-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture. Experimental Eye Research 1990, 51: 435-446. PMID: 2170160, DOI: 10.1016/0014-4835(90)90156-o.Peer-Reviewed Original ResearchConceptsBasolateral plasma membranePlasma membranePrimary cell culturesPlasma membrane proteinsFunctional tight junctionsCultured RPEChicken embryosMatrix-coated filtersExtracellular matrix coatingCell culturesMembrane proteinsVesicular stomatitis virusMorphological polarityApical poleCultured cellsApical membraneEngelbreth-HolmJunctional complexesSwarm tumorEmbryonic eyeStomatitis virusATPaseBasolateral membraneSerum-free mediumTight junctions