Depletion of CD4 T cells provides therapeutic benefits in aged mice after ischemic stroke
Harris NM, Roy-O'Reilly M, Ritzel RM, Holmes A, Sansing LH, O'Keefe LM, McCullough LD, Chauhan A. Depletion of CD4 T cells provides therapeutic benefits in aged mice after ischemic stroke. Experimental Neurology 2020, 326: 113202. PMID: 31954116, PMCID: PMC7059209, DOI: 10.1016/j.expneurol.2020.113202.Peer-Reviewed Original ResearchConceptsIP-10 levelsMiddle cerebral artery occlusionCD4 T cellsT cellsIschemic strokeStroke patientsIsotype controlBrain-infiltrating T cellsCD4 T-cell depletionCerebral artery occlusionT-cell depletionAged mouse modelAged male micePro-inflammatory factorsT cell dysfunctionPro-inflammatory cytokinesAnti-CD4 antibodiesAged stroke patientsIgG isotype controlCD4 treatmentDepletion antibodyArtery occlusionExperimental strokeStroke onsetSham surgeryAging exacerbates neutrophil pathogenicity in ischemic stroke
Roy-O'Reilly MA, Ahnstedt H, Spychala MS, Munshi Y, Aronowski J, Sansing LH, McCullough LD. Aging exacerbates neutrophil pathogenicity in ischemic stroke. Aging 2020, 12: 436-461. PMID: 31927534, PMCID: PMC6977697, DOI: 10.18632/aging.102632.Peer-Reviewed Original ResearchConceptsIschemic stroke patientsIschemic strokeNeutrophil-activating cytokineStroke patientsNeutrophil functionNeutrophil reactive oxygen speciesPoor post-stroke outcomesDepletion of neutrophilsPro-inflammatory functionsStrong risk factorExperimental mouse modelPost-stroke outcomesHigher stroke mortalityStroke outcomePoor outcomeFunctional outcomeStroke mortalityLong-term benefitsRisk factorsSpecific monoclonal antibodiesNeutrophil trafficTissue injuryYoung miceAged subjectsMouse model