2016
CJD and Scrapie Require Agent‐Associated Nucleic Acids for Infection
Botsios S, Manuelidis L. CJD and Scrapie Require Agent‐Associated Nucleic Acids for Infection. Journal Of Cellular Biochemistry 2016, 117: 1947-1958. PMID: 26773845, DOI: 10.1002/jcb.25495.Peer-Reviewed Original ResearchConceptsTSE agentsTransmissible spongiform encephalopathiesAdaptive immune responsesDegenerative brain changesInfectious particlesHost prion proteinGT1 neuronal cellsForms of PrPLymphoreticular tissuesBrain changesImmune responseTSE strainsNeuronal cellsNeurodegenerative diseasesLatent virusInfectivity assaysSpongiform encephalopathiesNucleic acid genomeTitersEpidemic spreadViral structuresPrion proteinHost proteinsVirusHost components
2014
Highly Infectious CJD Particles Lack Prion Protein but Contain Many Viral‐Linked Peptides by LC‐MS/MS
Kipkorir T, Tittman S, Botsios S, Manuelidis L. Highly Infectious CJD Particles Lack Prion Protein but Contain Many Viral‐Linked Peptides by LC‐MS/MS. Journal Of Cellular Biochemistry 2014, 115: 2012-2021. PMID: 24933657, PMCID: PMC7166504, DOI: 10.1002/jcb.24873.Peer-Reviewed Original ResearchConceptsSporadic CJDMouse brainTransmissible encephalopathiesNormal human brain samplesHost prion proteinHuman brain samplesSCJD brainsPrion proteinAmyloid pathologyAPP processingNew therapiesUninfected controlsBrain homogenatesBrain samplesCellular findingsDetectable PrPNeurodegenerative diseasesSheep scrapieInfectious titerBrainProteinase KCJDLC-MS/MSViral motifsInfectious form
2013
Infectious particles, stress, and induced prion amyloids
Manuelidis L. Infectious particles, stress, and induced prion amyloids. Virulence 2013, 4: 373-383. PMID: 23633671, PMCID: PMC3714129, DOI: 10.4161/viru.24838.Peer-Reviewed Original ResearchConceptsDiverse organismsInfectious TSE agentEnvironmental metagenomesTransmissible encephalopathiesHost prion proteinProtein misfoldingInfectious amyloidProtein modelingSusceptible PrP genotypesPrion amyloidInnate immune responsePrion proteinNeuronal agingDifferent amyloid proteinsTSE agentsMicrobial agentsNeurodegenerative diseasesPublic health measuresOrganismsBiological characteristicsNucleic acidsInfectious particlesAmyloid proteinProteinEndemic scrapie
2010
Agent-specific Shadoo Responses in Transmissible Encephalopathies
Miyazawa K, Manuelidis L. Agent-specific Shadoo Responses in Transmissible Encephalopathies. Journal Of Neuroimmune Pharmacology 2010, 5: 155-163. PMID: 20112073, PMCID: PMC2875883, DOI: 10.1007/s11481-010-9191-1.Peer-Reviewed Original ResearchConceptsAgent-specific differencesTransmissible spongiform encephalopathiesGT1 cellsTga20 miceTSE agentsHost prion proteinKuru agentRegional neuropathologyIntracellular amyloidSporadic CJDPrP changesTSE infectionUninfected controlsPrP levelsInfectious agentsAbnormal PrPTransmissible encephalopathiesMiceNeurodegenerative diseasesScrapie agentCJDInfectionInfected cellsSheep scrapieSpongiform encephalopathies
2009
The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents
Manuelidis L, Chakrabarty T, Miyazawa K, Nduom NA, Emmerling K. The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 13529-13534. PMID: 19633190, PMCID: PMC2715327, DOI: 10.1073/pnas.0905825106.Peer-Reviewed Original ResearchConceptsSporadic Creutzfeldt-Jakob diseaseCreutzfeldt-Jakob diseaseBovine spongiform encephalopathyBSE agentHuman sporadic Creutzfeldt-Jakob diseaseInfectious agentsEpidemic bovine spongiform encephalopathyTSE agentsScrapie agentTransmissible spongiform encephalopathy agentsSpongiform encephalopathy agentKuru agentLymphoreticular involvementBrain neuropathologySporadic CJDGT1 cellsNormal miceInfected humansNeurodegenerative diseasesDiseaseViral receptorsInfectious neurodegenerative diseasesSheep scrapieSpongiform encephalopathiesHigh levels