A high-throughput assay for directly monitoring nucleolar rRNA biogenesis
Bryant CJ, McCool MA, Abriola L, Surovtseva YV, Baserga SJ. A high-throughput assay for directly monitoring nucleolar rRNA biogenesis. Open Biology 2022, 12: 210305. PMID: 35078352, PMCID: PMC8790372, DOI: 10.1098/rsob.210305.Peer-Reviewed Original ResearchConceptsRibosome biogenesisRRNA biogenesisNucleolar functionSiRNA controlRNA polymerase 1Nucleolar protein fibrillarinPutative small molecule inhibitorsHigh-throughput primary screensHigh-throughput assaysSmall molecule inhibitorsProtein fibrillarinBiogenesisBMH-21Polymerase-1FibrillarinPrimary screenPrimary assayNucleoliAssaysMorphological alterationsRNAP1RibosomopathiesHitsBiological underpinningsDirect quantification