2021
Ovarian transplantation with robotic surgery and a neovascularizing human extracellular matrix scaffold: a case series in comparison to meta-analytic data
Oktay K, Marin L, Bedoschi G, Pacheco F, Sugishita Y, Kawahara T, Taylan E, Acosta C, Bang H. Ovarian transplantation with robotic surgery and a neovascularizing human extracellular matrix scaffold: a case series in comparison to meta-analytic data. Fertility And Sterility 2021, 117: 181-192. PMID: 34801235, PMCID: PMC8863399, DOI: 10.1016/j.fertnstert.2021.08.034.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultCohort StudiesCryopreservationExtracellular MatrixFemaleFertility PreservationHumansMeta-Analysis as TopicNeovascularization, PhysiologicOvaryPregnancyPregnancy RateRetrospective StudiesRobotic Surgical ProceduresTissue Culture TechniquesTissue ScaffoldsTransplantation, AutologousYoung AdultConceptsGraft longevityCase seriesOvarian functionLow-dose aspirinOvarian tissue transplantationOvarian cortical piecesLower embryo qualityOocytes/embryosNeonatal outcomesTransdermal estrogenChemotherapy exposureOvarian transplantationCortical piecesMeta-analytic dataLower abdomenHealthy childrenLive birthsMAIN OUTCOMEGraft vascularizationExtracellular matrix scaffoldsOvarian tissueAneuploidy rateFunction returnsEmbryo qualityMeta-analytic updateComparison of open and a novel closed vitrification system with slow freezing for human ovarian tissue cryopreservation
Sugishita Y, Taylan E, Kawahara T, Shahmurzada B, Suzuki N, Oktay K. Comparison of open and a novel closed vitrification system with slow freezing for human ovarian tissue cryopreservation. Journal Of Assisted Reproduction And Genetics 2021, 38: 2723-2733. PMID: 34398400, PMCID: PMC8581089, DOI: 10.1007/s10815-021-02297-9.Peer-Reviewed Original ResearchConceptsFollicle survivalCortical piecesPrimary folliclesHuman ovarian tissue cryopreservationOvarian tissue cryopreservationCell death pathway activationOvarian cortical piecesPrimordial follicle survivalPrimordial follicle densityHuman ovarian tissueClosed vitrification systemTissue cryopreservationDNA damageOvarian tissueHistological analysisPathway activationApoptosis rateDevice groupSlow freezingAssociation of Germline BRCA Pathogenic Variants With Diminished Ovarian Reserve: A Meta-Analysis of Individual Patient-Level Data
Turan V, Lambertini M, Lee D, Wang E, Clatot F, Karlan B, Demeestere I, Bang H, Oktay K. Association of Germline BRCA Pathogenic Variants With Diminished Ovarian Reserve: A Meta-Analysis of Individual Patient-Level Data. Journal Of Clinical Oncology 2021, 39: 2016-2024. PMID: 33891474, PMCID: PMC8260903, DOI: 10.1200/jco.20.02880.Peer-Reviewed Original ResearchConceptsIndividual patient-level dataSerum AMH levelsDiminished ovarian reservePatient-level dataAMH levelsOvarian reservePathogenic variantsGermline BRCA pathogenic variantLow serum AMH levelsAnti-Müllerian hormone levelsOral contraceptive pill useLower AMH levelsDecreased ovarian reserveBody mass indexContraceptive pill useBRCA pathogenic variantsEvaluable womenMass indexMean ageHormone levelsBreast cancerAffected womenPill useMeta-AnalysisYoung womenIndividual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation
Titus S, Szymanska K, Musul B, Turan V, Taylan E, Garcia- Milian R, Mehta S, Oktay K. Individual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation. Scientific Reports 2021, 11: 407. PMID: 33431979, PMCID: PMC7801500, DOI: 10.1038/s41598-020-79643-x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntineoplastic Combined Chemotherapy ProtocolsApoptosisCyclophosphamideDNA DamageFemaleGene Expression ProfilingHeterograftsHumansMiceMice, Inbred NODMice, SCIDOocytesOogenesisOvarian FollicleOvarian ReserveOvarySignal TransductionSingle-Cell AnalysisTranscriptomeYoung AdultConceptsPrimordial follicle oocytesFollicle lossGrowth activationHuman ovarian xenograft modelPI3K/PTEN/AktFollicle oocytesDNA damagePI3K/PTEN/Akt pathwayOvarian reserve depletionPrimordial follicle reservePTEN/AKT pathwayIngenuity Pathway AnalysisOvarian xenograft modelPTEN/AKTSevere DNA damageExpression of AktGonadotoxic chemotherapyAnti-apoptotic Bcl2Early menopauseFollicle reserveTranscriptomic analysisCyclophosphamide injectionHuman ovaryPhosphorylation statusRNA sequencing
2009
Stem cells and reproductive medicine.
Oktem O, Oktay K. Stem cells and reproductive medicine. Minerva Obstetrics And Gynecology 2009, 61: 247-52. PMID: 19745792.Peer-Reviewed Original Research
2008
Fertility Issues and Options in Young Women with Cancer
Oktay K, Sönmezer M. Fertility Issues and Options in Young Women with Cancer. Recent Results In Cancer Research 2008, 178: 203-224. PMID: 18080455, DOI: 10.1007/978-3-540-71274-9_18.Peer-Reviewed Original ResearchConceptsFemale cancersHematopoietic stem cell transplantationLong-term complicationsStem cell transplantationCurrent treatment modalitiesNew cancer casesMultiagent chemotherapyCell transplantationCure rateTreatment modalitiesCancer incidenceSurgical techniqueCancer casesCertain malignanciesRadiation therapyFertility issuesCancerYoung womenDeath rate
2001
Ovarian tissue cryopreservation and transplantation: preliminary findings and implications for cancer patients
Oktay K. Ovarian tissue cryopreservation and transplantation: preliminary findings and implications for cancer patients. Human Reproduction Update 2001, 7: 526-534. PMID: 11727860, DOI: 10.1093/humupd/7.6.526.Peer-Reviewed Original ResearchConceptsOvarian tissue cryopreservationTissue cryopreservationYoung womenFrozen-banked ovarian tissueBone marrow transplantationCancers of childrenAntral follicle developmentAggressive chemotherapyComplete remissionCancer treatment modalitiesMarrow transplantationBenign causesCancer patientsTreatment modalitiesTransplantation trialsFresh graftsXenograft studiesRodent studiesOocyte cryopreservationOvarian tissuePatientsFollicle developmentTransplantationCancer treatmentWomenA technique for laparoscopic transplantation of frozen-banked ovarian tissue
Oktay K, Aydin B, Karlikaya G. A technique for laparoscopic transplantation of frozen-banked ovarian tissue. Fertility And Sterility 2001, 75: 1212-1216. PMID: 11384651, DOI: 10.1016/s0015-0282(01)01776-9.Peer-Reviewed Original ResearchConceptsFrozen-banked ovarian tissueLaparoscopic transplantationOvarian tissueAcademic medical centerSlow-freezing techniqueMenopausal gonadotropinOvarian stimulationSpontaneous mensesOvarian fossaPelvic peritoneumOvarian functionLaparoscopic techniqueOvarian cortexMedical CenterMAIN OUTCOMEMicrosurgical reconstructionProgesterone productionBlood flowFollicle developmentTransplantationPatientsTissueMensesGonadotropinTransplant
2000
The re-incarnation of an old question— clomid effect on oocyte and embryo?
Oktay K, Berkowitz P, Berkus M, Schenken R, Brzyski R. The re-incarnation of an old question— clomid effect on oocyte and embryo? Fertility And Sterility 2000, 74: 422. PMID: 10970170, DOI: 10.1016/s0015-0282(00)00663-4.Peer-Reviewed Original ResearchOvarian Function after Transplantation of Frozen, Banked Autologous Ovarian Tissue
Oktay K, Karlikaya G. Ovarian Function after Transplantation of Frozen, Banked Autologous Ovarian Tissue. New England Journal Of Medicine 2000, 342: 1919. PMID: 10877641, DOI: 10.1056/nejm200006223422516.Peer-Reviewed Original ResearchTransplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice
Oktay K, Newton H, Gosden R. Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice. Fertility And Sterility 2000, 73: 599-603. PMID: 10689020, DOI: 10.1016/s0015-0282(99)00548-8.Peer-Reviewed Original ResearchConceptsOvarian tissueSCID miceFrozen-thawed human ovarian tissueFrozen-thawed ovarian tissueAutologous ovarian transplantationFrozen-thawed graftsHuman ovarian tissueFollicle growth initiationLong-term survivalWeeks of graftingHuman primordial folliclesFollicle countOvarian transplantationImmunodeficiency miceMAIN OUTCOMEHealthy folliclesPrimordial folliclesAcademic centersAnimal studiesFollicle stageAdvanced folliclesFolliclesGraftMiceNecrotic cells
1997
Ontogeny of Follicle-Stimulating Hormone Receptor Gene Expression in Isolated Human Ovarian Follicles
Oktay K. Ontogeny of Follicle-Stimulating Hormone Receptor Gene Expression in Isolated Human Ovarian Follicles. The Journal Of Clinical Endocrinology & Metabolism 1997, 82: 3748-3751. DOI: 10.1210/jc.82.11.3748.Peer-Reviewed Original ResearchOntogeny of Follicle-Stimulating Hormone Receptor Gene Expression in Isolated Human Ovarian Follicles1
Oktay K, Briggs D, Gosden R. Ontogeny of Follicle-Stimulating Hormone Receptor Gene Expression in Isolated Human Ovarian Follicles1. The Journal Of Clinical Endocrinology & Metabolism 1997, 82: 3748-3751. PMID: 9360535, DOI: 10.1210/jcem.82.11.4346.Peer-Reviewed Original ResearchConceptsMessenger ribonucleic acidPrimordial folliclesFollicle stageElective cesarean sectionHuman ovarian folliclesPreantral follicle developmentFSH receptor geneHormone receptor gene expressionReceptor gene expressionFollicle-stimulating hormone receptor gene expressionCesarean sectionRectus muscleHuman ovaryAntral folliclesFollicular growthFSH expressionHuman folliculogenesisOvarian folliclesOvarian tissueGranulosa cellsMultilaminar folliclesStromal tissueSingle follicleFollicle developmentFolliclesIsolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue
Oktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden R. Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue. Fertility And Sterility 1997, 67: 481-486. PMID: 9091334, DOI: 10.1016/s0015-0282(97)80073-8.Peer-Reviewed Original ResearchConceptsHuman ovarian tissuePrimordial folliclesOvarian tissueWhole folliclesElective cesarean sectionLeeds General InfirmaryCryopreserved ovarian tissueHuman primordial folliclesCesarean sectionHealthy womenOvarian biopsyFollicle countingGeneral InfirmaryMAIN OUTCOMEFollicle viabilityUltrastructural signsFrozen-thawed groupFolliclesFresh groupTissuePropidium iodideSuccinimidyl esterViability ratePercent viabilityOocytes
1996
Diminished α-inhibin messenger ribonucleic acid in in vitro fertilization-embryo transfer poor responders reflects declining follicle reserve**Presented at the 41st Annual Meeting of the Society for Gynecologic Investigation, Chicago, Illinois, March 22 to 26, 1994.
Burns W, Oktay K, Tekmal R, Nelson J, Schenken R. Diminished α-inhibin messenger ribonucleic acid in in vitro fertilization-embryo transfer poor responders reflects declining follicle reserve**Presented at the 41st Annual Meeting of the Society for Gynecologic Investigation, Chicago, Illinois, March 22 to 26, 1994. Fertility And Sterility 1996, 65: 394-399. PMID: 8566269, DOI: 10.1016/s0015-0282(16)58106-0.Peer-Reviewed Original ResearchConceptsPoor respondersGood respondersMessenger RNA levelsOvarian responsivenessIVF-ETMRNA levelsPg/Academic tertiary care institutionGranulosa cell samplesRNA levelsDay of hCGTertiary care institutionGranulosa cell functionTotal mRNAGynecologic InvestigationFollicle numberFollicle aspirationE2 concentrationsMessenger ribonucleic acidRespondersFolliclesCare institutionsCell functionDecreased poolRecent reports