2015
A guide to generating and using hiPSC derived NPCs for the study of neurological diseases.
Topol A, Tran N, Brennand K. A guide to generating and using hiPSC derived NPCs for the study of neurological diseases. Journal Of Visualized Experiments 2015, e52495. PMID: 25742222, PMCID: PMC4354663, DOI: 10.3791/52495.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsHiPSC neural progenitor cellsRapid genetic screeningPluripotent stem cellsCellular phenotypesDevelopmental eventsMolecular consequencesGene expressionNeurological diseasesFunctional neuronsStem cellsProgenitor cellsOnset of symptomsMolecular factorsFurther differentiationPost-mortem studiesDisease initiationGenetic screeningOxidative stressSymptom onsetDisease progressionHealthy controlsDiseaseCellsPatients
2014
Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia
Brennand K, Savas J, Kim Y, Tran N, Simone A, Hashimoto-Torii K, Beaumont K, Kim H, Topol A, Ladran I, Abdelrahim M, Matikainen-Ankney B, Chao S, Mrksich M, Rakic P, Fang G, Zhang B, Yates J, Gage F. Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia. Molecular Psychiatry 2014, 20: 361-368. PMID: 24686136, PMCID: PMC4182344, DOI: 10.1038/mp.2014.22.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntipsychotic AgentsCell DifferentiationCell MovementCells, CulturedFemaleGene ExpressionHumansMaleMiceMice, Inbred C57BLMice, TransgenicMitochondriaNeural Cell Adhesion MoleculesNeural Stem CellsOxidative StressPhenotypePluripotent Stem CellsProsencephalonProteomicsReactive Oxygen SpeciesSchizophreniaYoung AdultConceptsHiPSC neural progenitor cellsNeural progenitor cellsHuman-induced pluripotent stem cellsHiPSC-derived neuronsGene expressionGene expression comparisonsStable isotope labelingProteomic mass spectrometry analysisAbnormal gene expressionPluripotent stem cellsOxidative stressCytoskeletal remodelingMass spectrometry analysisCellular phenotypesExpression comparisonsDevelopmental mechanismsIsotope labelingPhenotypic differencesBrainSpan AtlasDisease predispositionAmino acidsScalable assayNPC phenotypeStem cellsProgenitor cells