Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line
Nillni E, Sevarino K, Jackson I. Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line. Endocrinology 1993, 132: 1260-1270. DOI: 10.1210/en.132.3.1260.Peer-Reviewed Original ResearchSodium dodecyl sulfate-polyacrylamide gel electrophoresisCryptic peptidesDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisAtT20 cell lineCell linesPulse-chase studiesN-terminal fragmentGel filtration chromatographyCarboxy-terminalCell extractsGel electrophoresisPulse-chaseTRH progenitor sequenceProgenitor sequencePosttranslational processingWestern blot analysisAmino acidsBiosynthetic precursorFiltration chromatographyBlot analysisTumor cell linesGln-His-Pro-GlySequenceProTRH-derived peptidesProcessing of proTRH to its intermediate products occurs before the packing into secretory granules of transfected AtT20 cells
Nillni E, Sevarino K, Jackson I. Processing of proTRH to its intermediate products occurs before the packing into secretory granules of transfected AtT20 cells. Endocrinology 1993, 132: 1271-1277. DOI: 10.1210/en.132.3.1271.Peer-Reviewed Original ResearchPulse-chase studiesProcessing of proTRHSecretory granulesAtT20 cellsAssay of marker enzymesPolyacrylamide gel electrophoresisPulse-chase experimentsCryptic peptidesC-terminal processing productsNitrogen cavitationSubcellular compartmentsSG fractionGel electrophoresisRER fractionPosttranslational processingSubcellular fractionsIntracellular compartmentsPurified antibodiesProTRH processingIntermediate processing productsGolgiIntact precursorMarker enzymesPeptideProcessing products