Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line
Nillni E, Sevarino K, Jackson I. Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line. Endocrinology 1993, 132: 1260-1270. DOI: 10.1210/en.132.3.1260.Peer-Reviewed Original ResearchSodium dodecyl sulfate-polyacrylamide gel electrophoresisCryptic peptidesDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisAtT20 cell lineCell linesPulse-chase studiesN-terminal fragmentGel filtration chromatographyCarboxy-terminalCell extractsGel electrophoresisPulse-chaseTRH progenitor sequenceProgenitor sequencePosttranslational processingWestern blot analysisAmino acidsBiosynthetic precursorFiltration chromatographyBlot analysisTumor cell linesGln-His-Pro-GlySequenceProTRH-derived peptides