1988
Photochemical crosslinking of bacteriophage T4 single‐stranded DNA‐binding protein (gp32) to oligo‐p(dT)8: Identification of phenylalanine‐183 as the site of crosslinking
Shamoo Y, Williams K, Konigsberg W. Photochemical crosslinking of bacteriophage T4 single‐stranded DNA‐binding protein (gp32) to oligo‐p(dT)8: Identification of phenylalanine‐183 as the site of crosslinking. Proteins Structure Function And Bioinformatics 1988, 4: 1-6. PMID: 3186689, DOI: 10.1002/prot.340040103.Peer-Reviewed Original ResearchMeSH KeywordsCross-Linking ReagentsDNA, Single-StrandedDNA, ViralDNA-Binding ProteinsMacromolecular SubstancesOligodeoxyribonucleotidesPhenylalaninePhotochemistryT-PhagesViral ProteinsConceptsCovalent bond formationAnion-exchange high-performance liquid chromatographyHigh-performance liquid chromatographyBond formationGas-phase sequencingLiquid chromatographyPhotochemical crosslinkingPhenylthiohydantoin derivativesSer-GlyTryptic peptidesUltraviolet irradiationTyr-AspUltraviolet lightCrosslinkingSer-AsnHigh affinityCleavage productsGln-ValGlu-SerPeptidesPhotolysisTrypsin cleavage productSingle tryptic peptideChromatographyComplexes
1986
Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA).
Chase J, Rabin B, Murphy J, Stone K, Williams K. Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA). Journal Of Biological Chemistry 1986, 261: 14929-14935. PMID: 3021756, DOI: 10.1016/s0021-9258(18)66806-1.Peer-Reviewed Original ResearchConceptsExonuclease VII activityLarge subunitStandard E. coli genetic mapE. coli genetic mapEscherichia coli exonuclease VIIDeletion mutant strainAmino acid sequenceGenetic mapGene productsAcid sequenceMutant strainActive enzymeCell extractsBase pairsGenesExonuclease VIIAmino acidsSubunitsProteinSequenceGuaBXseACloningPromoterMolecular weight