2024
Mouse Models Enable the Functional Investigation of Tertiary Lymphoid Structures in Cancer
Jeevanandam A, Yin Z, Connolly K, Joshi N. Mouse Models Enable the Functional Investigation of Tertiary Lymphoid Structures in Cancer. Methods In Molecular Biology 2024, 2864: 57-76. PMID: 39527217, DOI: 10.1007/978-1-0716-4184-2_4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDisease Models, AnimalHumansLungMiceNeoplasmsTertiary Lymphoid StructuresTumor MicroenvironmentConceptsTertiary lymphoid structuresTertiary lymphoid structure formationSecondary lymphoid organsLymphoid structuresMurine modelFeatures of tertiary lymphoid structuresFunction of tertiary lymphoid structuresMouse modelPersistent inflammatory stimulationAssociated with positive clinical outcomesTissue-specific regulatory mechanismsPositive clinical outcomesPrognostic significanceClinical outcomesGut environmentNonlymphoid tissuesLymphoid aggregatesLymphoid organsMouse lungCancer patientsGenetic sequencesInflammatory stimulationRegulatory mechanismsTherapeutic modulationClinical efforts
2021
A reservoir of stem-like CD8+ T cells in the tumor-draining lymph node preserves the ongoing anti-tumor immune response
Connolly KA, Kuchroo M, Venkat A, Khatun A, Wang J, William I, Hornick NI, Fitzgerald BL, Damo M, Kasmani MY, Cui C, Fagerberg E, Monroy I, Hutchins A, Cheung JF, Foster GG, Mariuzza DL, Nader M, Zhao H, Cui W, Krishnaswamy S, Joshi NS. A reservoir of stem-like CD8+ T cells in the tumor-draining lymph node preserves the ongoing anti-tumor immune response. Science Immunology 2021, 6: eabg7836. PMID: 34597124, PMCID: PMC8593910, DOI: 10.1126/sciimmunol.abg7836.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesFemaleImmunotherapyLung NeoplasmsLymph NodesLymphocyte ActivationMaleMiceMice, Inbred C57BLTumor MicroenvironmentConceptsTumor-specific CD8T cellsTumor microenvironmentOngoing anti-tumor immune responseChronic lymphocytic choriomeningitis virus (LCMV) infectionTumor-draining lymph nodesAnti-tumor immune responseLymphocytic choriomeningitis virus infectionIntratumoral T cellsEfficacy of immunotherapyT cell responsesTumor-draining lymphAntitumor T cellsT cell terminal differentiationStem-like CD8Immunologic shiftGene expression signaturesLymph nodesTerminal differentiationLung tumorsVirus infectionLung adenocarcinomaImmune responseCD8Cell responses
2019
Stereotactic Body Radiation and Interleukin-12 Combination Therapy Eradicates Pancreatic Tumors by Repolarizing the Immune Microenvironment
Mills B, Connolly K, Ye J, Murphy J, Uccello T, Han B, Zhao T, Drage M, Murthy A, Qiu H, Patel A, Figueroa N, Johnston C, Prieto P, Egilmez N, Belt B, Lord E, Linehan D, Gerber S. Stereotactic Body Radiation and Interleukin-12 Combination Therapy Eradicates Pancreatic Tumors by Repolarizing the Immune Microenvironment. Cell Reports 2019, 29: 406-421.e5. PMID: 31597100, PMCID: PMC6919969, DOI: 10.1016/j.celrep.2019.08.095.Peer-Reviewed Original ResearchConceptsStereotactic body radiation therapyMultiple preclinical mouse modelsPancreatic ductal adenocarcinoma patientsEffective neoadjuvant therapyAdvanced metastatic diseaseStereotactic body radiationDuctal adenocarcinoma patientsLate-stage diseaseInterferon-gamma productionBody radiation therapySystemic tumor immunityPreclinical mouse modelsNeoadjuvant approachNeoadjuvant therapyLiver metastasesMetastatic diseaseCD8 TTumor immunityAdenocarcinoma patientsImmune microenvironmentTumor reductionMS administrationTherapy resultsPancreatic tumorsGamma production
2018
Lineage Specifiers in Lung Cancer Are Ahead of Their TIME
Connolly KA, Fitzgerald B, Joshi NS. Lineage Specifiers in Lung Cancer Are Ahead of Their TIME. Immunity 2018, 49: 587-589. PMID: 30332625, DOI: 10.1016/j.immuni.2018.10.004.Commentaries, Editorials and LettersInvestigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma
Connolly KA, Nader M, Joshi N. Investigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma. Methods In Molecular Biology 2018, 1845: 259-273. PMID: 30141018, DOI: 10.1007/978-1-4939-8709-2_15.ChaptersConceptsTertiary lymphoid structuresLung adenocarcinomaLymphoid structuresImmune cellsFluorescence-activated cell sortingLung lobesTumor-associated tertiary lymphoid structuresHuman lung cancer patientsStrong antitumor immune responseAdvanced stage diseaseAntitumor immune responseRegulatory T cellsLung cancer patientsMurine lung adenocarcinomaFunctional immune systemAnimal tumor modelsLymph nodesCancer patientsAutochthonous tumorsT cellsImmune responseMouse modelTherapeutic interventionsImmune systemTumor growth