Intramolecular autoinhibition of checkpoint kinase 1 is mediated by conserved basic motifs of the C-terminal kinase–associated 1 domain
Emptage RP, Schoenberger MJ, Ferguson KM, Marmorstein R. Intramolecular autoinhibition of checkpoint kinase 1 is mediated by conserved basic motifs of the C-terminal kinase–associated 1 domain. Journal Of Biological Chemistry 2017, 292: 19024-19033. PMID: 28972186, PMCID: PMC5704483, DOI: 10.1074/jbc.m117.811265.Peer-Reviewed Original ResearchConceptsKA1 domainIntramolecular autoinhibitionSer/Thr proteinKinase 1N-terminal kinase domainChk1 kinase activityKey regulatory mechanismTerminal regulatory regionDNA damage repairSite-directed mutagenesisCheckpoint kinase 1Thr proteinChk1 kinaseHuman Chk1Truncation mutantsKinase domainRegulatory regionsKinase activityBasic residuesDamage repairRegulatory mechanismsStructural homologyResidue linkerCell cyclePrimary structure