1994
Parathyroid hormone-related protein: evidence for isoform- and tissue-specific posttranslational processing.
Yang KH, dePapp AE, Soifer NE, Dreyer BE, Wu TL, Porter SE, Bellantoni M, Burtis WJ, Insogna KL, Broadus AE. Parathyroid hormone-related protein: evidence for isoform- and tissue-specific posttranslational processing. Biochemistry 1994, 33: 7460-9. PMID: 8003511, DOI: 10.1021/bi00189a054.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCHO CellsChromatography, AffinityChromatography, High Pressure LiquidCricetinaeCricetulusCulture Media, ConditionedHumansImmunohistochemistryMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProtein Processing, Post-TranslationalProteinsTumor Cells, CulturedConceptsHormone-related proteinCarboxy-terminal fragmentCell linesTissue-specific posttranslational processingRat insulinoma cellsDistinct paracrineHuman PTHrP geneHumoral hypercalcemiaMalignant tumorsAutocrine roleRIN cell linesMidregion fragmentPTHrP isoformsMost human cell linesNonmalignant tissuesPhysiological rationalePTHrP geneInsulinoma cellsChinese hamster ovary cellsHuman cell linesHamster ovary cellsSecretory formCellsOvary cellsPosttranslational processing
1993
Human granulosa-luteal cells secrete parathyroid hormone-related protein in vivo and in vitro.
Gutmann JN, Burtis WJ, Dreyer BE, Andrade-Gordon P, Penzias AS, Polan ML, Insogna KL. Human granulosa-luteal cells secrete parathyroid hormone-related protein in vivo and in vitro. The Journal Of Clinical Endocrinology & Metabolism 1993, 76: 1314-1318. PMID: 8496323, DOI: 10.1210/jcem.76.5.8496323.Peer-Reviewed Original ResearchConceptsGranulosa-luteal cellsOvarian follicular fluidImmunoradiometric assayHuman granulosa-luteal cellsHuman ovarian follicular fluidCultured granulosa-luteal cellsParathyroid hormone-related proteinSubsequent prospective analysisAdenylate cyclase-stimulating activityHormone-related proteinCyclase-stimulating activityPresence of mRNAPossible paracrineShort-term culturePTHrP levelsNormal mammalian tissuesBasal secretionImmunohistochemical studyFollicular fluidAutocrine rolePTHrP immunoreactivityProspective analysisPTHrPPTHImmunoreactivity