2016
High-resolution mass spectrometry of small molecules bound to membrane proteins
Gault J, Donlan JA, Liko I, Hopper JT, Gupta K, Housden NG, Struwe WB, Marty MT, Mize T, Bechara C, Zhu Y, Wu B, Kleanthous C, Belov M, Damoc E, Makarov A, Robinson CV. High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature Methods 2016, 13: 333-336. PMID: 26901650, PMCID: PMC4856209, DOI: 10.1038/nmeth.3771.Peer-Reviewed Original ResearchMeSH KeywordsHumansLipidsMass SpectrometryMembrane ProteinsModels, MolecularPeptide FragmentsProtein BindingSmall Molecule Libraries
2013
Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins
Bhattacharyya M, Gupta K, Gowd KH, Balaram P. Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins. Molecular Omics 2013, 9: 1340-1350. PMID: 23467691, DOI: 10.1039/c3mb25534d.Peer-Reviewed Original ResearchMeSH KeywordsCystineDisulfidesLactalbuminMass SpectrometryNatriuretic PeptidesNeurotoxinsPeptide FragmentsProteinsRecombinant ProteinsConceptsMass spectrometric fragmentationDisulfide connectivityRapid mass spectrometric determinationPeptide toxinsNatural peptide toxinsMass spectrometric determinationCID fragmentationSpectrometric determinationNatural peptidesProteolytic nickProteolytic peptidesDirect fragmentationUnambiguous characterisationDisulfide linkagesSubsequent determinationSynthetic analoguesRapid screeningMS profilesDisulfide crosslinksStraightforward approachNative disulfidesDeterminationPeptidesFoldamersIntact polypeptide
2011
Mass spectrometric identification of an intramolecular disulfide bond in thermally inactivated triosephosphate isomerase from a thermophilic organism Methanocaldococcus jannaschii
Banerjee M, Gupta K, Balaram H, Balaram P. Mass spectrometric identification of an intramolecular disulfide bond in thermally inactivated triosephosphate isomerase from a thermophilic organism Methanocaldococcus jannaschii. Rapid Communications In Mass Spectrometry 2011, 25: 1915-1923. PMID: 21698673, DOI: 10.1002/rcm.5058.Peer-Reviewed Original ResearchConceptsMethanocaldococcus jannaschiiTriosephosphate isomeraseDisulfide bond formationIntramolecular disulfide bondsCysteine thiol groupsMonomer molecular massThree-dimensional structureThermophilic enzymesMass spectrometric identificationMS/MS analysisTetrameric enzymeTertiary structureMolecular massDisulfide bridgesDisulfide-bonded moleculesDisulfide bondsMonomeric massJannaschiiCircular dichroismComplete lossSpectrometric identificationIsomeraseEnzymeTryptic digestDa difference