Combined Electron Transfer Dissociation–Collision-Induced Dissociation Fragmentation in the Mass Spectrometric Distinction of Leucine, Isoleucine, and Hydroxyproline Residues in Peptide Natural Products
Gupta K, Kumar M, Chandrashekara K, Krishnan KS, Balaram P. Combined Electron Transfer Dissociation–Collision-Induced Dissociation Fragmentation in the Mass Spectrometric Distinction of Leucine, Isoleucine, and Hydroxyproline Residues in Peptide Natural Products. Journal Of Proteome Research 2011, 11: 515-522. PMID: 22111579, DOI: 10.1021/pr200091v.Peer-Reviewed Original ResearchConceptsIsobaric residuesMass spectrometric peptide sequencingNatural peptide librariesPeptide natural productsCrude peptide mixturesTandem mass spectrometric methodMass spectrometric distinctionMass spectrometric methodRadical ionsIsobaric leucineElectron transferCollisional activationDissociation fragmentationPeptide mixturesNatural productsNominal massSpectrometric methodLiquid chromatographyPeptide sequencingPresence of hydroxyprolineIonsPeptide libraryDifferent peptidesDissociation approachHydroxyproline residues