2023
ATG9 vesicles comprise the seed membrane of mammalian autophagosomes
Olivas T, Wu Y, Yu S, Luan L, Choi P, Guinn E, Nag S, De Camilli P, Gupta K, Melia T. ATG9 vesicles comprise the seed membrane of mammalian autophagosomes. Journal Of Cell Biology 2023, 222: e202208088. PMID: 37115958, PMCID: PMC10148236, DOI: 10.1083/jcb.202208088.Peer-Reviewed Original ResearchConceptsAtg9 vesiclesMammalian autophagosomesStyrene maleic acid lipid particlesLipid scramblase activityLC3-IIAutophagosomes formAutophagosome membraneMature autophagosomesScramblase activityAutophagosome formationAtg9Lipid transportMembrane growthAutophagosomesNanoscale organizationProtein-mediated transferProteinMembrane surface areaOrganellesVesiclesSeed membraneMembraneLipid particlesLipidsDifferent stagesDirect determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransporters
2022
Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation
Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Journal Of Cell Biology 2022, 221: e202207022. PMID: 36282247, PMCID: PMC9597353, DOI: 10.1083/jcb.202207022.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumLipid dropletsProtein-mediated transferLipid transport proteinsLipid droplet formationLD biologyMitochondrial proteinsSecretory pathwayMass spectrometry analysisTerminal domainMitochondrial morphologyTransport proteinsLipid transportersCellular membranesLD metabolismMembrane contactX-ray structureSpectrometry analysisOrganellesGlycerophospholipidsProteinHydrophobic cavityFatty acidsLipidsMembrane
2018
Identifying key membrane protein lipid interactions using mass spectrometry
Gupta K, Li J, Liko I, Gault J, Bechara C, Wu D, Hopper JTS, Giles K, Benesch JLP, Robinson CV. Identifying key membrane protein lipid interactions using mass spectrometry. Nature Protocols 2018, 13: 1106-1120. PMID: 29700483, PMCID: PMC6049616, DOI: 10.1038/nprot.2018.014.Peer-Reviewed Original Research
2017
The role of interfacial lipids in stabilizing membrane protein oligomers
Gupta K, Donlan JAC, Hopper JTS, Uzdavinys P, Landreh M, Struwe WB, Drew D, Baldwin AJ, Stansfeld PJ, Robinson CV. The role of interfacial lipids in stabilizing membrane protein oligomers. Nature 2017, 541: 421-424. PMID: 28077870, PMCID: PMC5501331, DOI: 10.1038/nature20820.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBinding SitesCardiolipinsCell MembraneEscherichia coliEscherichia coli ProteinsLigandsLipidsMass SpectrometryMembrane ProteinsModels, MolecularMolecular Dynamics SimulationMoritellaProtein MultimerizationProtein StabilityReceptors, G-Protein-CoupledSodium-Hydrogen ExchangersThermodynamicsThermus thermophilus
2016
High-resolution mass spectrometry of small molecules bound to membrane proteins
Gault J, Donlan JA, Liko I, Hopper JT, Gupta K, Housden NG, Struwe WB, Marty MT, Mize T, Bechara C, Zhu Y, Wu B, Kleanthous C, Belov M, Damoc E, Makarov A, Robinson CV. High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature Methods 2016, 13: 333-336. PMID: 26901650, PMCID: PMC4856209, DOI: 10.1038/nmeth.3771.Peer-Reviewed Original Research