2008
MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors
Lu J, Guo S, Ebert BL, Zhang H, Peng X, Bosco J, Pretz J, Schlanger R, Wang JY, Mak RH, Dombkowski DM, Preffer FI, Scadden DT, Golub TR. MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors. Developmental Cell 2008, 14: 843-853. PMID: 18539114, PMCID: PMC2688789, DOI: 10.1016/j.devcel.2008.03.012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Bone Marrow CellsCell DifferentiationCell LineageCells, CulturedErythroid CellsErythropoietinGene Expression RegulationGenes, ReporterHematopoietic Stem CellsHumansIntegrin beta3K562 CellsMegakaryocytesMiceMice, Inbred C57BLMicroRNAsModels, BiologicalPlatelet Membrane Glycoprotein IIbProto-Oncogene Proteins c-mybThrombopoietinConceptsMegakaryocyte-erythrocyte progenitorsLineage specificationTranscription factor MYBMiR-150Cell fateLineage fateRegenerative biologyErythroid cellsFunction experimentsMultipotent cellsMegakaryocytic lineageMiRNA expressionPrimary cellsCritical targetModel systemMicroRNAsProgenitorsFateRegulationCellsImportant participantsMYBLineagesMiRNAsBiology
2006
Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature
Hazony Y, Lu J, St. Hilaire C, Ravid K. Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature. Nucleic Acids Research 2006, 34: 4416-4428. PMID: 16936310, PMCID: PMC1636359, DOI: 10.1093/nar/gkl578.Peer-Reviewed Original ResearchConceptsCell-specific gene expressionNon-coding regionsRelated DNA sequencesDNA sequencesGene expressionMegakaryocytic lineageUnique transcription factorsCommon DNA sequenceNon-coding sequencesGroup of genesGene promoter sequencesMammalian genomesRegulatory sequencesTranscription factorsPromoter sequencesCross-species differencesGene promoterDNA signaturesLineagesSpecific sequencesCommon sequenceDNA samplesSequencePromoter groupExpression
2004
Properties of Ets-1 Binding to Chromatin and Its Effect on Platelet Factor 4 Gene Expression
Lu J, Pazin MJ, Ravid K. Properties of Ets-1 Binding to Chromatin and Its Effect on Platelet Factor 4 Gene Expression. Molecular And Cellular Biology 2004, 24: 428-441. PMID: 14673175, PMCID: PMC303331, DOI: 10.1128/mcb.24.1.428-441.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsChromatinDetergentsGene Expression RegulationHumansMegakaryocytesMiceNucleosomesPlatelet Factor 4Platelet Glycoprotein GPIb-IX ComplexPlatelet Membrane GlycoproteinsPromoter Regions, GeneticProto-Oncogene Protein c-ets-1Proto-Oncogene ProteinsProto-Oncogene Proteins c-etsRatsSarcosineTranscription FactorsConceptsEts-1 bindsPromoter activityLineage-specific factorsChromatin immunoprecipitation assaysEts-1 siteGlycoprotein IIbLineage-specific promotersNaked DNAGPIIb promoterTranscriptional regulationAccessory factorsPF4 promoterTranscription factorsHypersensitive sitesImmunoprecipitation assaysHematopoietic lineagesDNase IChromatinGene expressionPromoter activationPromoterProximal regionModel systemFactor 4ET-1
2002
A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation
Kaluzhny Y, Hechler B, Lu J, Nguyen HG, Cataldo LM, Ravid K. A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation. FEBS Letters 2002, 527: 279-283. PMID: 12220674, DOI: 10.1016/s0014-5793(02)03230-1.Peer-Reviewed Original Research3' Flanking Region3' Untranslated RegionsAnimalsCell DifferentiationCells, CulturedGlyceraldehyde-3-Phosphate DehydrogenasesMegakaryocytesMiceNeoplasm ProteinsPlatelet Factor 4Promoter Regions, GeneticProto-Oncogene ProteinsReceptors, CytokineReceptors, Purinergic P2Receptors, Purinergic P2Y1Receptors, ThrombopoietinRNA StabilityRNA, MessengerThrombopoietin
2001
Roads to polyploidy: The megakaryocyte example
Ravid K, Lu J, Zimmet JM, Jones MR. Roads to polyploidy: The megakaryocyte example. Journal Of Cellular Physiology 2001, 190: 7-20. PMID: 11807806, DOI: 10.1002/jcp.10035.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell Cycle ProteinsCell DifferentiationGene Expression RegulationHumansMegakaryocytesPolyploidyConceptsCell cycleHigher ploidyHaploid chromosome numberGroup of genesEndomitotic cell cycleChromosome numberMammalian cellsCell physiologyDifferent cell cyclesAnaphase BS phaseMultiple copiesCell typesPlatelet precursorsPolyploidyGenesMegakaryocytesPloidyPolyploidizationCytokinesisInsectsCellsMitosisPlantsPhysiology
1999
Mpl ligand enhances the transcription of the cyclin D3 gene: a potential role for Sp1 transcription factor.
Wang Z, Zhang Y, Lu J, Sun S, Ravid K. Mpl ligand enhances the transcription of the cyclin D3 gene: a potential role for Sp1 transcription factor. Blood 1999, 93: 4208-21. PMID: 10361118, DOI: 10.1182/blood.v93.12.4208.412k17_4208_4221.Peer-Reviewed Original ResearchConceptsProtein phosphatase 1Cyclin D3 promoterMpl ligandCyclin D3 geneTranscription factorsSp1-dependent genesD3 geneSp1 transcription factorForm of Sp1Basal promoter activityMegakaryocytic cell linesCyclin D3 proteinSp familySp1 proteinD3 gene expressionSp1 sitesPhosphatase 1Okadaic acidCyclin D3 gene expressionNuclear runSp1DNase IGene expressionPromoter activityPromoter region