2017
Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate
Wang N, Cheng J, Fan R, Lu J. Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate. Analyst 2017, 142: 3203-3211. PMID: 28765841, PMCID: PMC5605290, DOI: 10.1039/c7an00670e.Peer-Reviewed Original ResearchConceptsWhole cell lysatesSmall non-coding RNAsComplex regulatory networkMiRNA profilingCell lysatesPost-transcriptional levelIsogenic cell linesNon-coding RNAsAdaptor ligationLow quantity samplesGenome scaleRegulatory networksGlobal profilingMiRNA captureGene expressionExpression profilesHuman diseasesMiRNA expressionLibrary preparationMiRNA alterationsCell typesMiRNA releaseRNA purificationMulti-step purificationCell lines
2016
MicroRNAs in Control of Stem Cells in Normal and Malignant Hematopoiesis
Roden C, Lu J. MicroRNAs in Control of Stem Cells in Normal and Malignant Hematopoiesis. Current Stem Cell Reports 2016, 2: 183-196. PMID: 27547713, PMCID: PMC4988405, DOI: 10.1007/s40778-016-0057-1.Peer-Reviewed Original ResearchHematopoietic stem cellsStem cellsPost-transcriptional gene regulation mechanismSpecific gene expression programsPost-transcriptional gene expressionSmall non-coding RNAsGene expression programsLeukemia stem cellsGene regulation mechanismsFunction of miRNAsNon-coding RNAsStudy of miRNAsRole of miRNAsCell signaling pathwaysLSC biologyCancer stem cell conceptStem cell fieldEpigenetic machineryExpression programsHSC emergenceEpigenetic programsStem cell conceptEpigenetic regulatorsDifferentiated progenyHSC niche