Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State
Chen Z, Lu Y, Zhang K, Xiao Y, Lu J, Fan R. Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State. Advanced Science 2019, 6: 1801361. PMID: 31065513, PMCID: PMC6498135, DOI: 10.1002/advs.201801361.Peer-Reviewed Original ResearchImmune cellsLigand lipopolysaccharideTime pointsToll-like receptor 4 ligand lipopolysaccharideBasal stateIndividual immune cellsActivation stateHuman macrophage responseEffector responsesMultiple time pointsInflammatory programSingle-cell RNA sequencingMacrophage responsePathogenic challengeSecretion assaysCell populationsSame single cellTime courseHomogeneous cell populationSecretion analysisLongitudinal trackingRNA sequencingCellsHeterogeneous responseSequential measurements