2022
N 6-methyladenosine enhances post-transcriptional gene regulation by microRNAs
Kanoria S, Rennie WA, Carmack CS, Lu J, Ding Y. N 6-methyladenosine enhances post-transcriptional gene regulation by microRNAs. Bioinformatics Advances 2022, 2: vbab046. PMID: 35098135, PMCID: PMC8792947, DOI: 10.1093/bioadv/vbab046.Peer-Reviewed Original ResearchPost-transcriptional gene regulationMiRNA-binding sitesGene regulationMiRNA-mediated gene regulationEukaryotic messenger RNAsMiRNA-mediated regulationMiRNA-target bindingRNA-binding proteinMiRNA target sitesPost-transcriptional regulatorsPotential methylation sitesHigh GC contentHigh-throughput dataArgonaute proteinsEvolutionary conservationPrevalent modificationTarget mRNAsGC contentMethylation sitesTarget secondary structureGene expressionRNA structureSecondary structureMessenger RNAFunctional significance
2016
In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system
Narayanan A, Hill-Teran G, Moro A, Ristori E, Kasper DM, A. Roden C, Lu J, Nicoli S. In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system. Scientific Reports 2016, 6: 32386. PMID: 27572667, PMCID: PMC5004112, DOI: 10.1038/srep32386.Peer-Reviewed Original ResearchConceptsMiRNA familiesSingle guide RNAsMiRNA gene familiesHigher multicellular organismsMultiplexed CRISPR/Entire miRNA familiesMulticellular organismsMiRNA genesGene familySame physiological functionChromosomal locationPhylogenetic ancestorsGenomic sequencesCas9 nucleaseGuide RNACRISPR/Mutagenesis strategyNuclease systemPrimary sequenceVivo mutagenesisPhysiological functionsSecondary structureModel systemMiRNAsMutations