2020
Antimicrobial activity of plant extracts against carbapenem-producing Klebsiella pneumoniae and in vivo toxicological assessment
Vasconcelos N, Vaz M, Radai J, Kassuya C, Formagio A, Graciani F, Leal M, Oliveira R, da Silva K, Croda J, Simionatto S. Antimicrobial activity of plant extracts against carbapenem-producing Klebsiella pneumoniae and in vivo toxicological assessment. Journal Of Toxicology And Environmental Health Part A 2020, 83: 719-729. PMID: 32981476, DOI: 10.1080/15287394.2020.1824135.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsCarbapenemsKlebsiella pneumoniaeMaleMiceMicrobial Sensitivity TestsPlant ExtractsConceptsSwiss male miceMultidrug-resistant strainsSplenic phagocytosisMale miceAgar disk diffusionBeneficial effectsToxicological assessmentMicronucleus assayKlebsiella pneumoniaePlant extractsVivo toxicological assessmentDisk diffusionAntibioticsAntimicrobial activityMutagenic activityGlobal spreadExtractMolecular characterizationAssessmentAntibacterial activityActivityMiceActive productsSynergismSynergistic effects of Cinnamomum cassia L. essential oil in combination with polymyxin B against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens
Vasconcelos N, de Sá Queiroz J, da Silva K, de Paula Vasconcelos P, Croda J, Simionatto S. Synergistic effects of Cinnamomum cassia L. essential oil in combination with polymyxin B against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens. PLOS ONE 2020, 15: e0236505. PMID: 32701970, PMCID: PMC7377461, DOI: 10.1371/journal.pone.0236505.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsBacterial ProteinsBeta-LactamasesCinnamomum aromaticumDrug Resistance, Multiple, BacterialDrug SynergismHumansKlebsiella InfectionsKlebsiella pneumoniaeOils, VolatilePolymyxin BSerratia InfectionsSerratia marcescensSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationEffect of High vs Low Doses of Chloroquine Diphosphate as Adjunctive Therapy for Patients Hospitalized With Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection
Borba M, Val F, Sampaio V, Alexandre M, Melo G, Brito M, Mourão M, Brito-Sousa J, Baía-da-Silva D, Guerra M, Hajjar L, Pinto R, Balieiro A, Pacheco A, Santos J, Naveca F, Xavier M, Siqueira A, Schwarzbold A, Croda J, Nogueira M, Romero G, Bassat Q, Fontes C, Albuquerque B, Daniel-Ribeiro C, Monteiro W, Lacerda M. Effect of High vs Low Doses of Chloroquine Diphosphate as Adjunctive Therapy for Patients Hospitalized With Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection. JAMA Network Open 2020, 3: e208857. PMID: 32330277, DOI: 10.1001/jamanetworkopen.2020.8857.Peer-Reviewed Original ResearchConceptsHigh dosage groupSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionAcute respiratory syndrome coronavirus 2 infectionLow dosage groupSyndrome coronavirus 2 infectionCoronavirus 2 infectionChloroquine diphosphateDay 13COVID-19Phase IIb clinical trialNonsevere COVID-19Secondary end pointsSevere COVID-19High-dose groupSpecific antiviral therapyTertiary care facilityIIb clinical trialCoronavirus disease 2019More heart diseaseParticipants' clinical statusAdjunctive therapyAdult patientsIll patientsPrimary outcomeAntiviral therapyRisk factors for polymyxin-resistant carbapenemase-producing Enterobacteriaceae in critically ill patients: An epidemiological and clinical study
da Silva K, Baker S, Croda J, Nguyen T, Boinett C, Barbosa L, Tetila A, Simionatto S. Risk factors for polymyxin-resistant carbapenemase-producing Enterobacteriaceae in critically ill patients: An epidemiological and clinical study. International Journal Of Antimicrobial Agents 2020, 55: 105882. PMID: 31923570, DOI: 10.1016/j.ijantimicag.2020.105882.Peer-Reviewed Original ResearchConceptsRisk factorsPolymyxin-resistant strainsNeonatal patientsMortality rateNeonatal intensive care unitEnterobacteriaceae strainsOnly risk factorCentral venous cathetersIntensive care unitCase-control studyPotential risk factorsHigh mortality ratePolymyxin-susceptible strainsCarbapenemase-producing EnterobacteriaceaeCarbapenem exposureAdult patientsAminoglycoside useCarbapenem useRenal failureVenous cathetersIll patientsCare unitUnfavourable outcomePolymerase chain reactionUrinary catheterAntibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain
Vasconcelos N, Silva K, Croda J, Simionatto S. Antibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain. Revista Da Sociedade Brasileira De Medicina Tropical 2020, 53: e20200032. PMID: 33027413, PMCID: PMC7534965, DOI: 10.1590/0037-8682-0032-2020.Peer-Reviewed Original ResearchConceptsHigh antibiotic resistanceMultidrug resistance profilesTime-kill methodsBla OXA-1Multidrug-resistant bacteriaMinimum inhibitory concentrationBla TEMBla CTXAntibacterial activityInhibitory effectBroth microdilutionInhibitory concentrationAntibiotic resistanceK. aerogenesOXA-1KPC-2CarbapenemsCinnamomum cassia essential oilL. essential oilCassia essential oilEssential oilCTXActivity
2019
Origanum vulgare L. essential oil inhibits the growth of carbapenem-resistant gram-negative bacteria
Vasconcelos N, Croda J, Silva K, Motta M, Maciel W, Limiere L, Simionatto S. Origanum vulgare L. essential oil inhibits the growth of carbapenem-resistant gram-negative bacteria. Revista Da Sociedade Brasileira De Medicina Tropical 2019, 52: e20180502. PMID: 31271619, DOI: 10.1590/0037-8682-0502-2018.Peer-Reviewed Original ResearchConceptsCarbapenem-resistant strainsAlternative therapeutic optionTime-kill assaysMultidrug-resistant bacteriaTherapeutic optionsMinimum inhibitory concentrationAcinetobacter baumanniiCell countCarbapenemase productionKill assaysOriganum vulgare L. essential oilL. essential oilKlebsiella pneumoniaeInhibitory concentrationDrug developmentResistant bacteriaH treatmentSerratia marcescensAntimicrobial effectNegative bacteria
2018
A high mortality rate associated with multidrug-resistant Acinetobacter baumannii ST79 and ST25 carrying OXA-23 in a Brazilian intensive care unit
da Silva K, Maciel W, Croda J, Cayô R, Ramos A, de Sales R, Kurihara M, Vasconcelos N, Gales A, Simionatto S. A high mortality rate associated with multidrug-resistant Acinetobacter baumannii ST79 and ST25 carrying OXA-23 in a Brazilian intensive care unit. PLOS ONE 2018, 13: e0209367. PMID: 30592758, PMCID: PMC6310363, DOI: 10.1371/journal.pone.0209367.Peer-Reviewed Original ResearchMeSH KeywordsAcinetobacter baumanniiAcinetobacter InfectionsAdultAgedAged, 80 and overAnti-Bacterial AgentsBacterial ProteinsBeta-LactamasesBrazilCase-Control StudiesCross InfectionDrug Resistance, Multiple, BacterialFemaleHospital MortalityHumansIntensive Care UnitsMaleMicrobial Sensitivity TestsMiddle AgedConceptsOXA-23-producing A. baumanniiHigh mortality rateA. baumanniiMortality rateRisk factorsHealthcare-related risk factorsBrazilian intensive care unitsIntensive care unit patientsIntensive care unit settingCarbapenem-resistant Acinetobacter baumanniiISAba1 insertion sequenceA. baumannii infectionsCare unit patientsIntensive care unitBlaOXA-51 geneUse of cephalosporinsCase-control studyBaumannii infectionsUnit patientsCare unitNasogastric tubeTherapeutic optionsUnit settingNosocomial infectionsSuch infectionsAntibacterial mechanisms of cinnamon and its constituents: A review
Vasconcelos N, Croda J, Simionatto S. Antibacterial mechanisms of cinnamon and its constituents: A review. Microbial Pathogenesis 2018, 120: 198-203. PMID: 29702210, DOI: 10.1016/j.micpath.2018.04.036.Peer-Reviewed Original ResearchConceptsAntibacterial mechanismGram-negative bacteriaCell divisionMembrane porinsBiofilm formationPotential new sourceCell membraneResistant bacterial infectionsAnti-quorum sensing effectsPathogenic GramCurrent knowledgeBacteriaBacterial infectionsNew antibioticsCommercial antibioticsMulti-drug resistant bacterial infectionsSynergistic interactionTypes of mechanismsAntibacterial actionATPasesPorinCinnamic acidMechanismAntibacterial agentsGlobal health threat
2017
Anti-inflammatory, antimycobacterial and genotoxic evaluation of Doliocarpus dentatus
Ishikawa R, Leitão M, Kassuya R, Macorini L, Moreira F, Cardoso C, Coelho R, Pott A, Gelfuso G, Croda J, Oliveira R, Kassuya C. Anti-inflammatory, antimycobacterial and genotoxic evaluation of Doliocarpus dentatus. Journal Of Ethnopharmacology 2017, 204: 18-25. PMID: 28392458, DOI: 10.1016/j.jep.2017.04.004.Peer-Reviewed Original ResearchConceptsMinimal inhibitory concentrationDoliocarpus dentatusPeripheral leukocyte countAnti-inflammatory agentsMechanical hypersensitivityUrine retentionCarrageenan modelLeukocyte countOral administrationLeukocyte migrationInflammation processPhagocytic activityPhagocytosis testMycobacterium tuberculosisMicronucleus testSignificant decreaseEthanolic extractGenotoxic aspectsGenotoxic evaluationInhibitory concentrationAntimycobacterial activitySafe useBetulinic acidMagnetic resonanceCount
2015
Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital
Silva K, Cayô R, Carvalhaes C, Sacchi F, Rodrigues-Costa F, da Silva A, Croda J, Gales A, Simionatto S. Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital. Journal Of Clinical Microbiology 2015, 53: 2324-2328. PMID: 25878341, PMCID: PMC4473237, DOI: 10.1128/jcm.00727-15.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAnti-Bacterial AgentsBeta-Lactam ResistanceBeta-LactamasesBrazilCarbapenemsChild, PreschoolCross InfectionDisease OutbreaksDNA, BacterialFemaleGenes, BacterialHospitals, TeachingHumansInfantIntegronsMaleMicrobial Sensitivity TestsMiddle AgedMinocyclineMolecular Sequence DataPlasmidsSequence Analysis, DNASerratia InfectionsSerratia marcescensTigecycline