Genome-Wide Transposon Mutagenesis in Pathogenic Leptospira Species
Murray GL, Morel V, Cerqueira GM, Croda J, Srikram A, Henry R, Ko AI, Dellagostin OA, Bulach DM, Sermswan RW, Adler B, Picardeau M. Genome-Wide Transposon Mutagenesis in Pathogenic Leptospira Species. Infection And Immunity 2008, 77: 810-816. PMID: 19047402, PMCID: PMC2632054, DOI: 10.1128/iai.01293-08.Peer-Reviewed Original ResearchConceptsTransposon mutagenesisTransposon mutantsGenome-wide transposon mutagenesisL. interrogansTransposon insertion sitesNovel virulence factorsEssential genesHypothetical genesGene functionGenome sequenceTransposon insertionGene disruptionGenetic manipulationDifferent genesGenetic analysisHeme biosynthesisPathogenic Leptospira speciesMutantsSequence analysisGenesVirulence factorsInsertional hot spotsLocation of insertionGenomeMutagenesis