2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCalcium-Transporting ATPasesCell MembraneHeLa CellsHumansIntracellular MembranesProtein TransportSecretory PathwaySphingomyelinsTrans-Golgi NetworkConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivity
2014
Secretory cargo sorting at the trans-Golgi network
Kienzle C, von Blume J. Secretory cargo sorting at the trans-Golgi network. Trends In Cell Biology 2014, 24: 584-593. PMID: 24841758, DOI: 10.1016/j.tcb.2014.04.007.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsBiological TransportCell MembraneHumansIntracellular MembranesProtein TransportSecretory PathwayTrans-Golgi NetworkConceptsTrans-Golgi networkCytoplasmic actin cytoskeletonPlasma membrane deliverySubset of proteinsNovel sorting mechanismKey cellular eventsSecretory cargoActin cytoskeletonCellular eventsBinding proteinLysosomal hydrolasesSorting mechanismProteinNormal physiologySuccessful sortingRecent discoverySortingCab45CytoskeletonHydrolasesSPCA1ATPaseRegulationPhysiologyCargo
2012
Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast
Curwin AJ, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Molecular Biology Of The Cell 2012, 23: 2327-2338. PMID: 22553351, PMCID: PMC3374751, DOI: 10.1091/mbc.e11-09-0826.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsBlotting, WesternCathepsin ACell MembraneCofilin 1EndocytosisGlucan Endo-1,3-beta-D-GlucosidaseGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, FluorescenceMutationProtein TransportProton-Translocating ATPasesR-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrans-Golgi NetworkVacuolesConceptsTrans-Golgi networkLate Golgi membranesCarboxypeptidase YSecretory cargoGolgi membranesLate Golgi compartmentMechanisms of cargoSoluble secretory proteinsRole of cofilinATPase Pma1Yeast orthologueSynthetic sicknessProtein cofilinSpecific cargoMutant cellsMammalian cellsGolgi compartmentSorting defectsPlasma membraneSecretory proteinsGolgi apparatusATPase 1CofilinCell surfacePMR1
2009
Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network
von Blume J, Duran JM, Forlanelli E, Alleaume AM, Egorov M, Polishchuk R, Molina H, Malhotra V. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2009, 187: 1055-1069. PMID: 20026655, PMCID: PMC2806282, DOI: 10.1083/jcb.200908040.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkADF/cofilinActin-depolymerizing factorSecretory proteinsSecretory cargoCofilin knockdownMass spectrometry-based protein profilingMammalian tissue culture cellsGolgi-resident proteinsSorting of proteinsEndogenous secretory proteinsIntegral membrane proteinsSoluble secretory proteinsStable isotope labelingTissue culture cellsDrosophila melanogasterActin remodelingMammalian cellsMembrane proteinsGolgi membranesProtein profilingIsotope labelingCofilinCell surfaceAmino acids