2022
Quantification of Protein Exit at the Trans-Golgi Network
Tran M, Kim Y, von Blume J. Quantification of Protein Exit at the Trans-Golgi Network. Methods In Molecular Biology 2022, 2557: 583-594. PMID: 36512239, DOI: 10.1007/978-1-0716-2639-9_35.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkProtein exportSecretory pathwaySelective hooks (RUSH) systemCell biological approachesProtein of interestPulse-chase experimentsProtein exitProtein sortingExport kineticsCellular homeostasisProtein transportDifferent cargoesLive cellsBiological approachesRUSH systemProteinPathwayTraffickingHomeostasisExportCargoSortingCompartmentsCells
2019
Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network
von Blume J, Hausser A. Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network. FEBS Letters 2019, 593: 2412-2427. PMID: 31344259, PMCID: PMC8048779, DOI: 10.1002/1873-3468.13552.Peer-Reviewed Original Research
2010
Role of the Second Cysteine-rich Domain and Pro275 in Protein Kinase D2 Interaction with ADP-Ribosylation Factor 1, Trans-Golgi Network Recruitment, and Protein Transport
Pusapati GV, Krndija D, Armacki M, von Wichert G, von Blume J, Malhotra V, Adler G, Seufferlein T. Role of the Second Cysteine-rich Domain and Pro275 in Protein Kinase D2 Interaction with ADP-Ribosylation Factor 1, Trans-Golgi Network Recruitment, and Protein Transport. Molecular Biology Of The Cell 2010, 21: 1011-1022. PMID: 20089835, PMCID: PMC2836954, DOI: 10.1091/mbc.e09-09-0814.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecond cysteine-rich domainCysteine-rich domainProtein transportC1a domainPlasma membraneADP-ribosylation factor 1ADP-ribosylation factorPKD isoenzymesTGN membranesSecretory trafficPKD2 functionsSmall GTPaseC1b domainKey regulatorPKD2Transport carriersARF1Factor 1PKDPrecise mechanismMembraneRecruitmentDomainArf6