2023
Rediscovering the intricacies of secretory granule biogenesis
Campelo F, Tian M, von Blume J. Rediscovering the intricacies of secretory granule biogenesis. Current Opinion In Cell Biology 2023, 85: 102231. PMID: 37657367, DOI: 10.1016/j.ceb.2023.102231.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSG biogenesisRegulated secretionImmature SGsEssential cellular processesSecretory granulesSecretory granule biogenesisImmature secretory granulesCargo selectionCargo receptorsCellular processesGranule biogenesisGranule cargoCargo moleculesBiogenesisBiophysical mechanismsDisease mechanismsCellular communicationCargoCentral roleDiverse rangeNew lightTraffickingMechanismProtein
2022
Liquid–liquid phase separation facilitates the biogenesis of secretory storage granules
Parchure A, Tian M, Stalder D, Boyer C, Bearrows S, Rohli K, Zhang J, Rivera-Molina F, Ramazanov B, Mahata S, Wang Y, Stephens S, Gershlick D, von Blume J. Liquid–liquid phase separation facilitates the biogenesis of secretory storage granules. Journal Of Cell Biology 2022, 221: e202206132. PMID: 36173346, PMCID: PMC9526250, DOI: 10.1083/jcb.202206132.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkLiquid-liquid phase separationSecretory granulesClient moleculesSecretory storage granulesΒ-cellsMembrane traffickingCargo receptorsPlasma membranePancreatic β-cellsProcessing enzymesCell typesStorage granulesChromogranin proteinsBiogenesisAcidic pHTraffickingGranulesProteinEnzyme
2017
Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network
Blank B, von Blume J. Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network. European Journal Of Cell Biology 2017, 96: 383-390. PMID: 28372832, DOI: 10.1016/j.ejcb.2017.03.001.Peer-Reviewed Original ResearchConceptsMolecular functionsCargo moleculesCytoplasmic actin cytoskeletonSpecific cargo moleculesTransmembrane cargo receptorsTrans-Golgi networkMannose-6-phosphate receptorCargo receptorsGolgi networkActin cytoskeletonATPase SPCA1Secreted proteinsPlasma membraneSecretory proteinsExtracellular proteasesCab45Sorting mechanismSurface receptorsProteinExtracellular spaceSpecific carrierPossible roleFinal destinationSortingReceptors