2016
Calcium-Sensing Receptor Promotes Breast Cancer by Stimulating Intracrine Actions of Parathyroid Hormone–Related Protein
Kim W, Takyar FM, Swan K, Jeong J, VanHouten J, Sullivan C, Dann P, Yu H, Fiaschi-Taesch N, Chang W, Wysolmerski J. Calcium-Sensing Receptor Promotes Breast Cancer by Stimulating Intracrine Actions of Parathyroid Hormone–Related Protein. Cancer Research 2016, 76: 5348-5360. PMID: 27450451, PMCID: PMC5026591, DOI: 10.1158/0008-5472.can-15-2614.Peer-Reviewed Original ResearchConceptsMMTV-PyMT miceBreast cancer cellsCaSR activationBone metastasesBreast cancerInhibited tumor cell proliferationOsteolytic bone metastasesCancer cellsHuman breast cancer cell linesCalcium-sensing receptorHuman breast cancer cellsHormone-related proteinTransgenic mouse modelBreast cancer cell linesMMTV-PyMT transgenic mouse modelBreast cancer progressionTumor cell proliferationTumor cell growthCancer cell linesPTHrP levelsTissue-specific disruptionHigh extracellular concentrationsPTHrP productionCASR genePTHrP expression
2014
Deletion of the Nuclear Localization Sequences and C-Terminus of PTHrP Impairs Embryonic Mammary Development but also Inhibits PTHrP Production
Boras-Granic K, Dann P, VanHouten J, Karaplis A, Wysolmerski J. Deletion of the Nuclear Localization Sequences and C-Terminus of PTHrP Impairs Embryonic Mammary Development but also Inhibits PTHrP Production. PLOS ONE 2014, 9: e90418. PMID: 24785493, PMCID: PMC4006745, DOI: 10.1371/journal.pone.0090418.Peer-Reviewed Original ResearchConceptsMammary gland developmentType 1 PTH/PTHrP receptorC-terminusEmbryonic mammary gland developmentPTH/PTHrP receptorGland developmentPTHrP mRNA levelsNuclear localization signalNuclear localization sequenceHormone-related proteinEmbryonic mammary developmentMammary epithelial cellsLocalization signalNuclear functionsPTHrP productionPTHrP receptorAlternative animal modelLocalization sequencePTHR1 geneAnimal modelsEndocrine fashionMutant formsPTHrPDevelopmental defectsMesenchyme differentiationPeriosteal PTHrP regulates cortical bone modeling during linear growth in mice
Wang M, VanHouten JN, Nasiri AR, Tommasini SM, Broadus AE. Periosteal PTHrP regulates cortical bone modeling during linear growth in mice. Journal Of Anatomy 2014, 225: 71-82. PMID: 24762197, PMCID: PMC4073294, DOI: 10.1111/joa.12184.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsBone DevelopmentFibulaGene DeletionMiceMice, KnockoutParathyroid Hormone-Related ProteinPeriosteumRANK LigandTibiaConceptsMetaphyseal-diaphyseal junctionParathyroid hormone-related peptideEndocortical bone formationCD-1 miceNF-kB ligandHormone-related peptideMineral apposition rateCortical bone modelingEntire boneLinear growthAnteromedial cortexCKO miceProximal tibiaLateral tibiaReceptor activatorOC numberApposition rateOsteoclastic resorptionPTHrPBone modelingBone formationFibulaLong bonesMicePeriosteum
2013
Mammary-Specific Ablation of the Calcium-Sensing Receptor During Lactation Alters Maternal Calcium Metabolism, Milk Calcium Transport, and Neonatal Calcium Accrual
Mamillapalli R, VanHouten J, Dann P, Bikle D, Chang W, Brown E, Wysolmerski J. Mammary-Specific Ablation of the Calcium-Sensing Receptor During Lactation Alters Maternal Calcium Metabolism, Milk Calcium Transport, and Neonatal Calcium Accrual. Endocrinology 2013, 154: 3031-3042. PMID: 23782944, PMCID: PMC3749485, DOI: 10.1210/en.2012-2195.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiological TransportBone DevelopmentBone ResorptionCalciumCrosses, GeneticFemaleGene Expression RegulationLactationLactoglobulinsMammary Glands, AnimalMiceMice, KnockoutMice, TransgenicMilkParathyroid HormoneParathyroid Hormone-Related ProteinReceptors, Calcium-SensingRNA, MessengerConceptsRenal calcium excretionCalcium excretionCalcium metabolismBone resorptionCalcium transportMilk calciumMammary glandAccelerated bone resorptionMammary-specific ablationMaternal calcium metabolismDietary calcium intakeCalcium sensing receptorCalcium-sensing receptorSecretion of PTHrPSkeletal calcium storesMilk PTHrPCalcium intakePTH secretionPTHrP secretionMaternal boneBone turnoverBone metabolismOnset of lactationMaternal circulationSystemic calciumThe calcium-sensing receptor in the breast
VanHouten JN, Wysolmerski JJ. The calcium-sensing receptor in the breast. Best Practice & Research Clinical Endocrinology & Metabolism 2013, 27: 403-414. PMID: 23856268, PMCID: PMC3713417, DOI: 10.1016/j.beem.2013.02.011.BooksMeSH KeywordsAnimalsBreastEpithelial CellsFemaleHumansLactationMammary Glands, AnimalMammary Glands, HumanParathyroid Hormone-Related ProteinReceptors, Calcium-SensingSignal TransductionConceptsG-protein usageBreast cancer cellsCalcium-sensing receptorCalcium metabolismCancer cellsParathyroid hormone-related protein levelsBreast cellsMaternal calcium metabolismOsteolytic skeletal metastasesSystemic calcium metabolismEpithelial cellsNormal breast epithelial cellsFeed-forward cycleMalignant breast cellsNormal breast cellsBreast epithelial cellsSkeletal metastasesPTHrP productionPTHrP expressionMammary epithelial cellsCaSRCalcium transportProtein levelsTransport of calciumSupply of calcium
2012
PTHrP regulates the modeling of cortical bone surfaces at fibrous insertion sites during growth
Wang M, VanHouten JN, Nasiri AR, Johnson RL, Broadus AE. PTHrP regulates the modeling of cortical bone surfaces at fibrous insertion sites during growth. Journal Of Bone And Mineral Research 2012, 28: 598-607. PMID: 23109045, PMCID: PMC3574208, DOI: 10.1002/jbmr.1801.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone DevelopmentFibrocartilageMiceModels, BiologicalParathyroid Hormone-Related ProteinConceptsCortical bone surfaceCortical surfaceParathyroid hormone-related proteinLong bonesHormone-related proteinMedial collateral ligamentBone cell activityBone surfaceLong bone growthTendon insertionForme frusteCollateral ligamentFibrous enthesesInitial genetic evidenceCell activityInsertion sitePTHrPConditional deletionLinear growthEnthesesLigamentPeriosteal componentBiomechanical controlBoneFruste
2011
Parathyroid Hormone-Related Protein Is Not Required for Normal Ductal or Alveolar Development in the Post-Natal Mammary Gland
Boras-Granic K, VanHouten J, Hiremath M, Wysolmerski J. Parathyroid Hormone-Related Protein Is Not Required for Normal Ductal or Alveolar Development in the Post-Natal Mammary Gland. PLOS ONE 2011, 6: e27278. PMID: 22087279, PMCID: PMC3210770, DOI: 10.1371/journal.pone.0027278.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFemaleGene ExpressionLactationMammary Glands, AnimalMiceMice, TransgenicParathyroid Hormone-Related ProteinPregnancyTissue DistributionConceptsMammary glandPTHrP expressionAlveolar developmentMyoepithelial cellsMaternal calcium homeostasisOverexpression of PTHrPRole of PTHrPExpression of PTHrPEmbryonic mammary budPTHrP deficiencyMouse mammary glandParathyroid hormoneTransgenic miceDuctal developmentCalcium homeostasisAlveolar cellsPTHrPPubertal developmentMMTV-CrePost-natal mammary glandMammary developmentMammary budPostnatal mammary glandCre transgenePTHrP gene
2010
Increased PTHrP and Decreased Estrogens Alter Bone Turnover but Do Not Reproduce the Full Effects of Lactation on the Skeleton
Ardeshirpour L, Brian S, Dann P, VanHouten J, Wysolmerski J. Increased PTHrP and Decreased Estrogens Alter Bone Turnover but Do Not Reproduce the Full Effects of Lactation on the Skeleton. Endocrinology 2010, 151: 5591-5601. PMID: 21047946, PMCID: PMC2999486, DOI: 10.1210/en.2010-0566.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesBone DensityEstrogensFemaleFertility Agents, FemaleLactationLeuprolideMiceOvariectomyParathyroid Hormone-Related ProteinConceptsBone mineral densityBone lossEstrogen deficiencyMineral densityInfusion of PTHrPReversible bone lossLevels of estrogenCombination of OVXLeuprolide treatmentCentral hypogonadismGnRH agonistMaternal skeletonBone turnoverOsmotic minipumpsOsteoclast numberBone metabolismBone resorptionSurgical ovariectomyNulliparous micePTHrPOVXInfusionEstrogenLeuprolideModest decline
2008
Switching of G-protein Usage by the Calcium-sensing Receptor Reverses Its Effect on Parathyroid Hormone-related Protein Secretion in Normal Versus Malignant Breast Cells*
Mamillapalli R, VanHouten J, Zawalich W, Wysolmerski J. Switching of G-protein Usage by the Calcium-sensing Receptor Reverses Its Effect on Parathyroid Hormone-related Protein Secretion in Normal Versus Malignant Breast Cells*. Journal Of Biological Chemistry 2008, 283: 24435-24447. PMID: 18621740, PMCID: PMC2528989, DOI: 10.1074/jbc.m801738200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone NeoplasmsBreast NeoplasmsCell Line, TumorCyclic AMPCyclic AMP-Dependent Protein KinasesFemaleGTP-Binding Protein alpha SubunitsHumansHypercalcemiaLactationMammary Glands, AnimalMammary Neoplasms, AnimalMAP Kinase Signaling SystemMiceNeoplasm MetastasisNeoplasm ProteinsParathyroid Hormone-Related ProteinPregnancyReceptors, Calcium-SensingType C PhospholipasesConceptsG-protein usagePTHrP productionMammary epithelial cellsBreast cancer cellsBreast cellsMCF-7 cellsParathyroid hormone-related protein secretionParathyroid hormone-related proteinParathyroid hormone secretionCancer cellsMCF-7 human breast cancer cellsCalcium-sensing receptorHuman breast cancer cellsHormone-related proteinNormal mammary epithelial cellsRegulation of calciumMalignant breast cellsNormal breast cellsG protein-coupled receptorsG protein couplingBone metastasesPTHrP secretionHormone secretionBone metabolismBreast cancer
2005
The calcium-sensing receptor regulates PTHrP production and calcium transport in the lactating mammary gland
Ardeshirpour L, Dann P, Pollak M, Wysolmerski J, VanHouten J. The calcium-sensing receptor regulates PTHrP production and calcium transport in the lactating mammary gland. Bone 2005, 38: 787-793. PMID: 16377269, DOI: 10.1016/j.bone.2005.11.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumFemaleHaplotypesLactationMammary Glands, AnimalMiceMice, KnockoutMilkParathyroid Hormone-Related ProteinReceptors, Calcium-SensingConceptsCalcium-sensing receptorPTHrP productionMammary epithelial cellsMammary glandCalcium transportEpithelial cellsCalcium homeostasisParathyroid hormone-related proteinParathyroid hormone secretionProduction of PTHrPHormone-related proteinSystemic calcium homeostasisExtracellular calcium ionsTransepithelial calcium transportG protein-coupled receptorsHormone secretionParathyroid glandsBone massCalcium handlingRenal tubulesTransient lossGlandGenetic confirmationReceptorsSecretionHypercalcemia of Malignancy due to Ectopic Transactivation of the Parathyroid Hormone Gene
VanHouten JN, Yu N, Rimm D, Dotto J, Arnold A, Wysolmerski JJ, Udelsman R. Hypercalcemia of Malignancy due to Ectopic Transactivation of the Parathyroid Hormone Gene. The Journal Of Clinical Endocrinology & Metabolism 2005, 91: 580-583. PMID: 16263810, DOI: 10.1210/jc.2005-2095.Peer-Reviewed Original ResearchMeSH KeywordsAgedDNA MethylationDNA, NeoplasmFatal OutcomeFemaleGene ExpressionHumansHypercalcemiaHyperparathyroidismNeuroendocrine TumorsPancreatic NeoplasmsParathyroid GlandsParathyroid Hormone-Related ProteinPromoter Regions, GeneticReverse Transcriptase Polymerase Chain ReactionTranscriptional ActivationCalcium Sensing by the Mammary Gland
VanHouten JN. Calcium Sensing by the Mammary Gland. Journal Of Mammary Gland Biology And Neoplasia 2005, 10: 129-139. PMID: 16025220, DOI: 10.1007/s10911-005-5396-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone NeoplasmsBreastBreast NeoplasmsCalciumFemaleHumansLactationMammary Glands, AnimalParathyroid Hormone-Related ProteinPregnancyReceptors, Calcium-SensingConceptsMammary glandExtracellular calcium-sensing receptorMaternal calcium homeostasisOsteolytic bone metastasesCalcium-sensing receptorHormone-related proteinNeoplastic mammary epithelial cellsBreast cancer cellsBone metastasesMammary epithelial cellsCalcium homeostasisCalcium fluxEpithelial cellsMilk secretionCancer cellsCalcium sensingImportant entityGlandCellsMetastasisPTHrPSecretionReceptors
2004
The calcium-sensing receptor regulates mammary gland parathyroid hormone–related protein production and calcium transport
VanHouten J, Dann P, McGeoch G, Brown EM, Krapcho K, Neville M, Wysolmerski JJ. The calcium-sensing receptor regulates mammary gland parathyroid hormone–related protein production and calcium transport. Journal Of Clinical Investigation 2004, 113: 598-608. PMID: 14966569, PMCID: PMC338258, DOI: 10.1172/jci18776.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related protein productionCalcium-sensing receptorMammary epithelial cellsEpithelial cellsCalcium transportMilk calcium contentExtracellular calcium concentrationSecretion of calciumTranscellular calcium transportPTHrP secretionPTHrP productionExtracellular calciumTransfer of calciumCalcium contentMammary glandCalcium concentrationProtein concentrationAvailability of calciumHomeostatic systemConcentration of calciumMilk osmolalitySecretionCalciumReceptorsThree-dimensional culture system
2003
Mammary-specific deletion of parathyroid hormone–related protein preserves bone mass during lactation
VanHouten JN, Dann P, Stewart AF, Watson CJ, Pollak M, Karaplis AC, Wysolmerski JJ. Mammary-specific deletion of parathyroid hormone–related protein preserves bone mass during lactation. Journal Of Clinical Investigation 2003, 112: 1429-1436. PMID: 14597768, PMCID: PMC228471, DOI: 10.1172/jci19504.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone DensityBone ResorptionCalcitriolCalciumFemaleHomeostasisIntegrasesLactationLactoglobulinsMammary Glands, AnimalMiceParathyroid Hormone-Related ProteinPromoter Regions, GeneticViral ProteinsConceptsBone lossBone turnoverMammary epithelial cellsParathyroid hormone-related proteinMammary glandMammary-specific deletionPTHrP-knockout miceRapid bone lossEpithelial cellsDihydroxy vitamin DNegative calcium balanceHormone-related proteinPreserves bone massAbsence of PTHrPExpression of CreUrinary cAMPVitamin DBone resorptionLate pregnancyBone massPTHrP mRNACalcium balanceKnockout micePTHrP proteinMammary development
2001
Temporally regulated overexpression of parathyroid hormone-related protein in the mammary gland reveals distinct fetal and pubertal phenotypes
Dunbar ME, Dann P, Brown CW, Van Houton J, Dreyer B, Philbrick WP, Wysolmerski JJ. Temporally regulated overexpression of parathyroid hormone-related protein in the mammary gland reveals distinct fetal and pubertal phenotypes. Journal Of Endocrinology 2001, 171: 403-416. PMID: 11739006, DOI: 10.1677/joe.0.1710403.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell DivisionEmbryonic and Fetal DevelopmentFemaleGene Expression Regulation, DevelopmentalMammary Glands, AnimalMiceMice, TransgenicMorphogenesisParathyroid Hormone-Related ProteinPhenotypeProteinsTetracyclineConceptsParathyroid hormone-related proteinHormone-related proteinPTHrP overexpressionMammary glandLobuloalveolar developmentDuctal elongationMammary developmentOverexpression of PTHrPDouble transgenic miceTransgenic mice resultsTerminal end budsEpithelial cell apoptosisPubertal phenotypesDuctal branchingEmbryonic mammary developmentTransgenic miceMice resultsPTHrPEnd budsDecrease apoptosisCell apoptosisBasal rateDuctal morphogenesisCell proliferationDuctal tree