2001
pp60c-src Modulates Microvascular Endothelial Phenotype and in Vitro Angiogenesis
Marx M, Warren S, Madri J. pp60c-src Modulates Microvascular Endothelial Phenotype and in Vitro Angiogenesis. Experimental And Molecular Pathology 2001, 70: 201-213. PMID: 11417999, DOI: 10.1006/exmp.2001.2358.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueAnimalsBecaplerminCell DivisionEndothelium, VascularGenes, srcGenetic VectorsMaleMicrocirculationMoloney murine leukemia virusNeovascularization, PhysiologicPlatelet-Derived Growth FactorProto-Oncogene Proteins c-sisProto-Oncogene Proteins pp60(c-src)RatsRecombinant ProteinsSignal TransductionTransfectionConceptsC-Src mutantC-SrcTwo-dimensional cultureThree-dimensional cultureWild-type c-SrcC-Src kinase activityC-Src tyrosine kinaseC-Src associatesC-src proteinPlatelet-derived growth factor receptorV-SrcPDGF signalCytoskeletal organizationGrowth factor receptorKinase activityCell shapeTyrosine kinaseVitro AngiogenesisTube-like structuresCell morphologyFactor receptorTube formationMutantsRegulatory effectsOverexpression
1997
Nitric oxide synthase inhibitors attenuate transforming-growth-factor-beta 1-stimulated capillary organization in vitro.
Papapetropoulos A, Desai KM, Rudic RD, Mayer B, Zhang R, Ruiz-Torres MP, García-Cardeña G, Madri JA, Sessa WC. Nitric oxide synthase inhibitors attenuate transforming-growth-factor-beta 1-stimulated capillary organization in vitro. American Journal Of Pathology 1997, 150: 1835-44. PMID: 9137106, PMCID: PMC1858220.Peer-Reviewed Original ResearchConceptsNitric oxideL-NAMETube formationNOS isoformsNitric oxide synthase inhibitorL-nitro-arginine methylesterOxide synthase inhibitorNO donor sodium nitroprussideRole of NOTranscriptase-polymerase chain reactionExcess L-arginineDonor sodium nitroprussideSoluble guanylate cyclaseWestern blot analysisEndothelial cell proliferationNOS blockadeCapillary tube formationEndothelial NOSSodium nitroprussideSynthase inhibitorL-arginineMicrovascular ECsAutocrine productionGuanylate cyclaseCapillary organization
1995
Human capillary endothelial cells from abdominal wall adipose tissue: Isolation using an anti-pecam antibody
Springhorn J, Madri J, Squinto S. Human capillary endothelial cells from abdominal wall adipose tissue: Isolation using an anti-pecam antibody. In Vitro Cellular & Developmental Biology - Animal 1995, 31: 473-481. PMID: 8589892, DOI: 10.1007/bf02634261.Peer-Reviewed Original ResearchMeSH KeywordsAbdomenAdipose TissueAnimalsAntibodies, MonoclonalAntigens, Differentiation, MyelomonocyticCell Adhesion MoleculesCell SeparationCell Transformation, ViralCells, CulturedEndothelium, VascularGrowth HormoneHumansLectinsPlant LectinsPlatelet Endothelial Cell Adhesion Molecule-1Vascular Cell Adhesion Molecule-1Von Willebrand FactorConceptsCapillary endothelial cellsHuman capillary endothelial cellsAnti-PECAM antibodyEndothelial cellsCellular adhesion moleculesVon Willebrand factorAdipose tissueDensity lipoproteinAdhesion molecule antibodyGrowth hormoneFlow cytometryMolecule antibodiesAdhesion moleculesAntibodiesWillebrand factorPassage sixNormal patternAntibody methodCell isolatesImmunofluorescent labelingAgglutinin lectinType I
1994
Modulation of platelet-derived growth factor receptor expression in microvascular endothelial cells during in vitro angiogenesis.
Marx M, Perlmutter R, Madri J. Modulation of platelet-derived growth factor receptor expression in microvascular endothelial cells during in vitro angiogenesis. Journal Of Clinical Investigation 1994, 93: 131-139. PMID: 7506710, PMCID: PMC293745, DOI: 10.1172/jci116936.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueAnimalsCell DivisionCells, CulturedElectrophoresis, Polyacrylamide GelEndothelium, VascularEpididymisImmunoblottingKineticsMaleMicrocirculationMolecular WeightNeovascularization, PathologicPlatelet-Derived Growth FactorRatsReceptors, Platelet-Derived Growth FactorRecombinant ProteinsTime FactorsConceptsMicrovascular endothelial cellsEndothelial cellsPDGF receptorReceptor expressionPlatelet-derived growth factor receptor expressionGrowth factor receptor expressionPDGF receptor expressionFactor receptor expressionPDGF receptor alphaReceptor surface expressionEndothelial cell growthNovel therapeutic applicationsCell growthSuramin treatmentProliferative responseEndothelial cell phenotypeReceptor alphaPDGF isoformsPDGF-AAInhibited proliferationReceptor regulationPDGF-BBPDGF-ABAngiogenesisCell proliferation