2017
MMP-2: A modulator of neuronal precursor activity and cognitive and motor behaviors
Li Q, Michaud M, Shankar R, Canosa S, Schwartz M, Madri JA. MMP-2: A modulator of neuronal precursor activity and cognitive and motor behaviors. Behavioural Brain Research 2017, 333: 74-82. PMID: 28666838, DOI: 10.1016/j.bbr.2017.06.041.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell MovementCell ProliferationCells, CulturedCognitionExploratory BehaviorGene Expression RegulationMatrix Metalloproteinase 2MiceMice, Inbred C57BLMice, KnockoutMotor ActivityNerve Tissue ProteinsNeural Stem CellsNeurogenesisOncogene Protein v-aktProliferating Cell Nuclear AntigenReceptors, CXCR4Spatial LearningConceptsNeural precursor cellsBroad substrate specificityNeurosphere formationAdherent neurospheresSecondary neurosphere formationNPC activitySubstrate specificityNPC numberCell surface moleculesZinc-containing enzymesAkt activationAbsence of MMP2Cell typesExtracellular matrixActivity assaysPrecursor cellsImportant roleNPC migrationMatrix metalloproteinase2Surface moleculesExpressionKO miceBioactive moleculesNestin expressionMMP2
2008
TNF-&agr; Induces MMP2 Gelatinase Activity and MT1-MMP Expression in an In Vitro Model of Nucleus Pulposus Tissue Degeneration
Séguin CA, Pilliar RM, Madri JA, Kandel RA. TNF-&agr; Induces MMP2 Gelatinase Activity and MT1-MMP Expression in an In Vitro Model of Nucleus Pulposus Tissue Degeneration. Spine 2008, 33: 356-365. PMID: 18277865, DOI: 10.1097/brs.0b013e3181642a5e.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleElectrophoretic Mobility Shift AssayExtracellular Signal-Regulated MAP KinasesGene ExpressionImmunoblottingIn Vitro TechniquesIntervertebral DiscLuciferasesMatrix Metalloproteinase 14Matrix Metalloproteinase 2Reverse Transcriptase Polymerase Chain ReactionTumor Necrosis Factor-alphaUp-RegulationConceptsMMP-2 gelatinase activityERK MAPK pathwayTranscriptional activationMT1-MMPElectrophoretic mobility shift assaysMT1-MMP promoterMMP-2 geneMobility shift assaysSignal transduction mechanismsProtein levelsERK 1/2 activationNP tissuesTranscription factorsShift assaysMT1-MMP expressionReporter constructsTNF-alpha inductionERK MAPKGelatinase activityLuciferase constructEgr-1TNF-alpha treatmentMMP-2 activationSP-1Transduction mechanisms
2007
Loss of MMP-2 disrupts skeletal and craniofacial development and results in decreased bone mineralization, joint erosion and defects in osteoblast and osteoclast growth
Mosig RA, Dowling O, DiFeo A, Ramirez MC, Parker IC, Abe E, Diouri J, Al Aqeel AA, Wylie JD, Oblander SA, Madri J, Bianco P, Apte SS, Zaidi M, Doty SB, Majeska RJ, Schaffler MB, Martignetti JA. Loss of MMP-2 disrupts skeletal and craniofacial development and results in decreased bone mineralization, joint erosion and defects in osteoblast and osteoclast growth. Human Molecular Genetics 2007, 16: 1113-1123. PMID: 17400654, PMCID: PMC2576517, DOI: 10.1093/hmg/ddm060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArthritisBone and BonesBone RemodelingCalcification, PhysiologicCell ProliferationCells, CulturedCraniofacial AbnormalitiesGene DeletionHumansImmunohistochemistryJointsMatrix Metalloproteinase 2MiceMice, KnockoutOsteoblastsOsteoclastsReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingTime FactorsTomography, X-Ray ComputedConceptsMMP2-/- miceMMP-2Arthritis syndromeArticular cartilage destructionOsteoclast growthBone mineral densityDays of lifeWeeks of lifeWeeks of ageMMP-2 overexpressionJoint erosionsBone lossCartilage destructionNormal cell numbersPathophysiological mechanismsOsteoclast numberVivo physiological roleMineral densityControl littermatesAnatomical distributionBone disordersMurine modelMineralization defectMulticentric osteolysisDisease pathogenesis
2004
MMP‐2 null mice exhibit an early onset and severe experimental autoimmune encephalomyelitis due to an increase in MMP‐9 expression and activity
Esparza J, Kruse M, Lee J, Michaud M, Madri JA. MMP‐2 null mice exhibit an early onset and severe experimental autoimmune encephalomyelitis due to an increase in MMP‐9 expression and activity. The FASEB Journal 2004, 18: 1682-1691. PMID: 15522913, DOI: 10.1096/fj.04-2445com.Peer-Reviewed Original ResearchMeSH KeywordsAge of OnsetAnimalsBasement MembraneBlood-Brain BarrierBrainCD3 ComplexCell MovementEncephalomyelitis, Autoimmune, ExperimentalEnzyme ActivationGene Expression Regulation, EnzymologicMatrix Metalloproteinase 14Matrix Metalloproteinase 2Matrix Metalloproteinase 9Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMice, KnockoutT-Lymphocyte SubsetsT-LymphocytesConceptsMMP-2 KO miceExperimental autoimmune encephalomyelitisMMP-9 expressionAutoimmune encephalomyelitisEarly onsetWT miceKO miceMMP-9Bone marrowSevere experimental autoimmune encephalomyelitisMMP-2 null miceSimilar early onsetWT bone marrowClinical disease scoresMMP-2-deficient miceKO bone marrowMatrix metalloproteinase-2MT1-MMP expressionEndothelial cell monolayersSevere diseaseDisease scoreMetalloproteinase-2Collagen type IVMMP-2Matrix metalloproteinase
2003
Transcriptional Up-regulation of Endothelial Cell Matrix Metalloproteinase-2 in Response to Extracellular Cues Involves GATA-2*
Han X, Boyd PJ, Colgan S, Madri JA, Haas TL. Transcriptional Up-regulation of Endothelial Cell Matrix Metalloproteinase-2 in Response to Extracellular Cues Involves GATA-2*. Journal Of Biological Chemistry 2003, 278: 47785-47791. PMID: 14512418, DOI: 10.1074/jbc.m309482200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernBlotting, WesternCell NucleusCells, CulturedCollagenCOS CellsDNA-Binding ProteinsEndothelial CellsExtracellular MatrixGATA2 Transcription FactorGelatinGenes, ReporterLuciferasesMatrix Metalloproteinase 2MicrocirculationMolecular Sequence DataNeovascularization, PhysiologicPhenotypePromoter Regions, GeneticProtein BindingRatsTranscription FactorsTranscription, GeneticTranscriptional ActivationTransfectionUp-RegulationElevated glucose inhibits VEGF-A–mediated endocardial cushion formation
Enciso JM, Gratzinger D, Camenisch TD, Canosa S, Pinter E, Madri JA. Elevated glucose inhibits VEGF-A–mediated endocardial cushion formation. Journal Of Cell Biology 2003, 160: 605-615. PMID: 12591918, PMCID: PMC2173755, DOI: 10.1083/jcb.200209014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis Inducing AgentsAnimalsCell MovementCell SizeCells, CulturedCulture TechniquesDipeptidesEmbryo, MammalianEndocardial Cushion DefectsFemaleGlucoseHeartMaleMatrix Metalloproteinase 2MiceMorphogenesisMyocardiumPlatelet Endothelial Cell Adhesion Molecule-1Protease InhibitorsRecombinant Fusion ProteinsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1ConceptsEpithelial-mesenchymal transformationEndocardial cushion formationPlatelet endothelial cell adhesion molecule-1Cushion formationPECAM-1-positive endothelial cellsSingle cell motilityMMP-2 expressionMorphogenesis resultsHigh glucose-induced inhibitionCell motilityEndothelial cellsBlocks invasionMatrix metalloproteinase-2 expressionEndocardial cellsExtracellular matrixLack of invasionEndothelial cell adhesion molecule-1Mesenchymal cellsMyocardial VEGFMMP-2 inductionMetalloproteinase-2 expressionVEGF-A165ExpressionGrowth factorVascular endothelial growth factor
2000
Matrix metalloproteinase activity is required for activity-induced angiogenesis in rat skeletal muscle
Haas T, Milkiewicz M, Davis S, Zhou A, Egginton S, Brown M, Madri J, Hudlicka O. Matrix metalloproteinase activity is required for activity-induced angiogenesis in rat skeletal muscle. AJP Heart And Circulatory Physiology 2000, 279: h1540-h1547. PMID: 11009439, DOI: 10.1152/ajpheart.2000.279.4.h1540.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCapillariesCell DivisionDipeptidesElectric StimulationImmunohistochemistryMatrix Metalloproteinase 2Matrix Metalloproteinase InhibitorsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMicroscopy, ElectronMotor ActivityMuscle, SkeletalNeovascularization, PhysiologicProtease InhibitorsRatsRNA, MessengerConceptsMembrane proteinsBasement membrane proteinsEndothelial cell sprout formationRat skeletal muscleSkeletal muscleMatrix metalloproteinasesMembrane type 1Inflammation-mediated angiogenesisPhysiological angiogenesisBasement membraneCell proliferationMMP proteolysisProtein levelsProteolysisSprout formationMajor classesCritical roleProteinMatrix metalloproteinase activityMetalloproteinase activityProliferationAngiogenesisNew capillariesMembraneMMP inhibitionDistinct roles for matrix metalloproteinase-2 and α4 integrin in autoimmune T cell extravasation and residency in brain parenchyma during experimental autoimmune encephalomyelitis
Graesser D, Mahooti S, Madri J. Distinct roles for matrix metalloproteinase-2 and α4 integrin in autoimmune T cell extravasation and residency in brain parenchyma during experimental autoimmune encephalomyelitis. Journal Of Neuroimmunology 2000, 109: 121-131. PMID: 10996214, DOI: 10.1016/s0165-5728(00)00275-7.Peer-Reviewed Original ResearchConceptsMatrix metalloproteinase-2Auto-reactive T cellsExpression of alpha4T cellsMetalloproteinase-2Human multiple sclerosisExperimental autoimmune encephalomyelitisT cell extravasationMMP-2 inductionCentral nervous systemAutoimmune encephalomyelitisMultiple sclerosisAutoimmune diseasesBrain parenchymaNervous systemΑ4 integrinAlpha4 integrinsCell extravasationIndependent roleEAEAlpha4Basement membrane matrixInductionDistinct rolesIntegrins
1999
Extracellular Matrix-Driven Matrix Metalloproteinase Production in Endothelial Cells Implications for Angiogenesis
Haas T, Madri J. Extracellular Matrix-Driven Matrix Metalloproteinase Production in Endothelial Cells Implications for Angiogenesis. Trends In Cardiovascular Medicine 1999, 9: 70-77. PMID: 10578520, DOI: 10.1016/s1050-1738(99)00014-6.Peer-Reviewed Original ResearchConceptsInterstitial matrix moleculesCell-extracellular matrix interactionsBasement membrane matrixMatrix metalloproteinasesTranscriptional activationBlood vessel growthMatrix interactionsNew blood vessel growthMatrix moleculesCell surfaceExtracellular matrixMT1-MMPAdjacent cellsCell interactionsMatrix metalloproteinase productionCurrent understandingVessel growthParticular matrix metalloproteinasesEndothelial cell interactionsPotential roleAngiogenesisMetalloproteinase productionBasement membraneMMP-2Signaling
1998
The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis.
Graesser D, Mahooti S, Haas T, Davis S, Clark R, Madri J. The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis. Laboratory Investigation 1998, 78: 1445-58. PMID: 9840619.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDCell AdhesionCell MembraneCell MovementCells, CulturedClone CellsEncephalomyelitis, Autoimmune, ExperimentalEndothelium, VascularGelatinasesIntegrin alpha4Matrix Metalloproteinase 14Matrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMice, Inbred StrainsProtease InhibitorsTissue Inhibitor of Metalloproteinase-2T-LymphocytesTransfectionConceptsExperimental autoimmune encephalomyelitisT cell clonesRecombinant vascular cell adhesion molecule-1Autoreactive T cell clonesAlpha4 integrinsMMP-2EAE inductionAutoimmune encephalomyelitisMyelin basic protein-reactive T cell clonesDisease processMatrix metalloproteinaseVascular cell adhesion molecule-1Cell adhesion molecule-1Human multiple sclerosisSusceptible mouse strainsAdhesion molecule-1T cell transmigrationMatrix metalloproteinase-2Microvascular endothelial cellsAlpha4 integrin expressionExpression of alpha4Adoptive transferMultiple sclerosisMouse modelEndothelial cell layerThree-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*
Haas T, Davis S, Madri J. Three-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*. Journal Of Biological Chemistry 1998, 273: 3604-3610. PMID: 9452488, DOI: 10.1074/jbc.273.6.3604.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCollagenDrug CombinationsEndothelium, VascularEnzyme ActivationEnzyme InhibitorsGelatinasesLamininMatrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PhysiologicProtein ConformationProteoglycansRatsRats, Sprague-DawleyRNA, MessengerSignal TransductionTissue Inhibitor of Metalloproteinase-2ConceptsMT1-MMPTwo-dimensional cultureEndothelial cellsThree-dimensional collagen matrixEndothelial cell organizationEndothelial cell angiogenesisEndothelial cell migrationCell organizationCoordinate expressionPutative activatorMT1-MMP activationMMP-2Matrix metalloproteinasesCell migrationFunctional roleMatrix interactionsMatrix remodelingCollagen matrixCell angiogenesisEndothelial cell networksMMP-2 proteinProtein
1996
The roles of adhesion molecules and proteinases in lymphocyte transendothelial migration
Madri J, Graesser D, Haas T. The roles of adhesion molecules and proteinases in lymphocyte transendothelial migration. Biochemistry And Cell Biology 1996, 74: 749-757. PMID: 9164645, DOI: 10.1139/o96-082.Peer-Reviewed Original Research