1997
Involvement of endothelial PECAM-1/CD31 in angiogenesis.
DeLisser H, Christofidou-Solomidou M, Strieter R, Burdick M, Robinson C, Wexler R, Kerr J, Garlanda C, Merwin J, Madri J, Albelda S. Involvement of endothelial PECAM-1/CD31 in angiogenesis. American Journal Of Pathology 1997, 151: 671-7. PMID: 9284815, PMCID: PMC1857836.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin-Converting Enzyme InhibitorsAnimalsAntibodies, MonoclonalCells, CulturedCollagenCorneaDrug CombinationsEndotheliumFibroblast Growth Factor 2HumansLamininMiceMice, Inbred C57BLNeovascularization, PhysiologicPlatelet Endothelial Cell Adhesion Molecule-1ProteoglycansRatsRats, Sprague-DawleyTransforming Growth Factor betaConceptsCell-cell adhesion moleculesEndothelial cell-cell adhesion moleculesBasic fibroblast growth factorRat capillary endothelial cellsPECAM-1Adhesion moleculesHuman PECAM-1Murine PECAM-1Endothelial cellsFibroblast growth factorAdhesion receptorsProcess of angiogenesisPECAM-1/CD31Tube formationAdhesive interactionsVessel growthGrowth factorCapillary endothelial cellsPolyclonal antibodiesRat corneal neovascularizationAngiogenesisCorneal neovascularizationCellsMurine modelMonoclonal antibodiesNitric oxide synthase inhibitors attenuate transforming-growth-factor-beta 1-stimulated capillary organization in vitro.
Papapetropoulos A, Desai KM, Rudic RD, Mayer B, Zhang R, Ruiz-Torres MP, García-Cardeña G, Madri JA, Sessa WC. Nitric oxide synthase inhibitors attenuate transforming-growth-factor-beta 1-stimulated capillary organization in vitro. American Journal Of Pathology 1997, 150: 1835-44. PMID: 9137106, PMCID: PMC1858220.Peer-Reviewed Original ResearchConceptsNitric oxideL-NAMETube formationNOS isoformsNitric oxide synthase inhibitorL-nitro-arginine methylesterOxide synthase inhibitorNO donor sodium nitroprussideRole of NOTranscriptase-polymerase chain reactionExcess L-arginineDonor sodium nitroprussideSoluble guanylate cyclaseWestern blot analysisEndothelial cell proliferationNOS blockadeCapillary tube formationEndothelial NOSSodium nitroprussideSynthase inhibitorL-arginineMicrovascular ECsAutocrine productionGuanylate cyclaseCapillary organization
1996
Role of plasminogen activator inhibitor in the reciprocal regulation of bovine aortic endothelial and smooth muscle cell migration by TGF-beta 1.
Petzelbauer E, Springhorn J, Tucker A, Madri J. Role of plasminogen activator inhibitor in the reciprocal regulation of bovine aortic endothelial and smooth muscle cell migration by TGF-beta 1. American Journal Of Pathology 1996, 149: 923-31. PMID: 8780396, PMCID: PMC1865168.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell MovementCells, CulturedEndothelium, VascularExtracellular Matrix ProteinsMuscle, Smooth, VascularPlasminogen Activator Inhibitor 1Transforming Growth Factor betaVitronectinConceptsVascular cell typesCell typesSmooth muscle cellsPlasminogen activator inhibitorMuscle cellsAnti-catalytic antibodyActivator inhibitorExtracellular matrix componentsMigratory rateVascular smooth muscle cellsEndothelial cellsSmooth muscle cell migrationUrokinase plasminogen activatorMigratory behaviorReciprocal regulationMuscle cell migrationStable transfectantsCell migrationExtracellular matrixIntegrin synthesisMatrix componentsMigratory responseProteolytic phenotypeProteolytic activityGrowth factorModulation of transforming growth factor beta receptor levels on microvascular endothelial cells during in vitro angiogenesis.
Sankar S, Mahooti-Brooks N, Bensen L, McCarthy T, Centrella M, Madri J. Modulation of transforming growth factor beta receptor levels on microvascular endothelial cells during in vitro angiogenesis. Journal Of Clinical Investigation 1996, 97: 1436-1446. PMID: 8617876, PMCID: PMC507203, DOI: 10.1172/jci118565.Peer-Reviewed Original Research
1995
Expression of Transforming Growth Factor Type III Receptor in Vascular Endothelial Cells Increases Their Responsiveness to Transforming Growth Factor β2 ∗
Sankar S, Mahooti-Brooks N, Centrella M, McCarthy T, Madri J. Expression of Transforming Growth Factor Type III Receptor in Vascular Endothelial Cells Increases Their Responsiveness to Transforming Growth Factor β2 ∗. Journal Of Biological Chemistry 1995, 270: 13567-13572. PMID: 7768960, DOI: 10.1074/jbc.270.22.13567.Peer-Reviewed Original ResearchConceptsTGF beta 2Type II receptorBovine aortic endothelial cellsTGF beta 1Receptor proteinType III receptorII receptorsBeta 2TGF-beta type III receptorBeta 1Serine-threonine kinaseInhibitor-1 proteinPlasminogen activator inhibitor-1 proteinEndothelial cellsTGF beta sGrowth factor betaGrowth factor β2Inhibition of migrationVascular endothelial cellsSignaling capacityType IAortic endothelial cellsReceptor cDNAReceptor complexProtein
1993
Characterization of distinct functional domains of transforming growth factor beta.
Burmester J, Qian S, Roberts A, Huang A, Amatayakul-Chantler S, Suardet L, Odartchenko N, Madri J, Sporn M. Characterization of distinct functional domains of transforming growth factor beta. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 8628-8632. PMID: 7690965, PMCID: PMC47411, DOI: 10.1073/pnas.90.18.8628.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAlpha-MacroglobulinsAmino Acid SequenceAnimalsCattleCHO CellsCricetinaeDose-Response Relationship, DrugEndothelium, VascularHeartHumansKineticsMiceMolecular Sequence DataReceptors, Cell SurfaceReceptors, Transforming Growth Factor betaRecombinant Fusion ProteinsRecombinant ProteinsTransforming Growth Factor betaUmbilical VeinsConceptsGrowth factor betaFactor betaHuman colorectal cancer cell linesColorectal cancer cell linesLS513 cellsCancer cell linesTGF-beta moleculesCell linesAlphaBiological potencyBetaCellsCertain cellsAmino acidsPotencyDistinct functional domainsAdditional amino acidsDifferent isoformsIsoformsDistinct isoformsFunctional domainsChimeric proteinFibronectin expression correlates with U937 cell adhesion to migrating bovine aortic endothelial cells in vitro.
Hauser I, Setter E, Bell L, Madri J. Fibronectin expression correlates with U937 cell adhesion to migrating bovine aortic endothelial cells in vitro. American Journal Of Pathology 1993, 143: 173-80. PMID: 7686342, PMCID: PMC1886955.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell AdhesionCell MovementCells, CulturedDrug ResistanceEndothelium, VascularFibronectinsFluorescent Antibody TechniqueHumansIntegrinsLeukemia, Promyelocytic, AcuteNeomycinProto-Oncogene MasProto-Oncogene Proteins pp60(c-src)Transforming Growth Factor betaTumor Cells, CulturedConceptsCell adhesionU937 cell adhesionEndothelial cell populationSRC proto-oncogeneEndothelial cellsCell populationsExtracellular matrix componentsEndothelial cell migrationBovine aortic endothelial cellsExtracellular matrix synthesisDenudation injuryFibronectin depositionCell migrationProto-oncogeneVascular endothelial cellsU937 cellsAortic endothelial cellsAmount of fibronectinMatrix componentsMigratory responseEndothelial cell monolayersMatrix synthesisCellsCell monolayersAdhesion
1992
Differential modulation of vascular cell integrin and extracellular matrix expression in vitro by TGF‐β1 correlates with reciprocal effects on cell migration+
Basson C, Kocher O, Basson M, Asis A, Madri J. Differential modulation of vascular cell integrin and extracellular matrix expression in vitro by TGF‐β1 correlates with reciprocal effects on cell migration+. Journal Of Cellular Physiology 1992, 153: 118-128. PMID: 1522126, DOI: 10.1002/jcp.1041530116.Peer-Reviewed Original ResearchIndependent modulation of enterocyte migration and proliferation by growth factors, matrix proteins, and pharmacologic agents in an in vitro model of mucosal healing.
Basson M, Modlin I, Flynn S, Jena B, Madri J. Independent modulation of enterocyte migration and proliferation by growth factors, matrix proteins, and pharmacologic agents in an in vitro model of mucosal healing. Surgery 1992, 112: 299-307; discussion 307-8. PMID: 1353641.Peer-Reviewed Original ResearchConceptsMucosal healingPharmacologic agentsGrowth factorEGF-stimulated proliferationCaco-2 enterocytesHuman Caco-2 enterocytesInhibited basalAlpha 2 integrin subunitMatrix proteinsEnterocyte migrationCollagen IInhibitor genisteinIntegrin subunitsHealingIndomethacinProliferationTyrosine kinaseEGFLamininBasalGenisteinAltered organizationIndependent modulationIdentification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells.
Qian S, Burmester J, Merwin J, Madri J, Sporn M, Roberts A. Identification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 6290-6294. PMID: 1631120, PMCID: PMC49486, DOI: 10.1073/pnas.89.14.6290.Peer-Reviewed Original ResearchConceptsEndothelial cellsFetal bovine heart endothelial cellsMicrovascular endothelial cellsGrowth factor betaHeart endothelial cellsAortic endothelial cellsBovine aortic endothelial cellsFactor betaType 1Amino acids 40Beta moleculesInhibition of growthAmino acids 1Biological potencyCellsGreater activityModulation of vascular cell behavior by transforming growth factors β
Madri J, Bell L, Merwin J. Modulation of vascular cell behavior by transforming growth factors β. Molecular Reproduction And Development 1992, 32: 121-126. PMID: 1637550, DOI: 10.1002/mrd.1080320207.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEndothelium, VascularHumansMuscle, Smooth, VascularTransforming Growth Factor betaConceptsVascular cell typesCell typesCellular responsesCell surfaceTGF-beta receptorsVascular cell behaviorGrowth factor βDistinct bioassaysDifferent isoformsCell migrationCell behaviorInhibition of proliferationVascular cell responsesSmooth muscle cellsUnique binding profileAngiogenic assaysIsoformsBinding profileCell populationsBovine aortic endothelialFactor βMuscle cellsType IBASMCsAortic endothelial
1991
Vascular cell responses to a hybrid Transforming Growth Factor-Beta molecule
Merwin J, Tucker A, Madisen L, Purchio A, Madri J. Vascular cell responses to a hybrid Transforming Growth Factor-Beta molecule. Biochemical And Biophysical Research Communications 1991, 175: 589-595. PMID: 1708238, DOI: 10.1016/0006-291x(91)91606-d.Peer-Reviewed Original ResearchVascular Cell Responses to TGF-β3 Mimic Those of TGF-β1 in vitro
Merwin J, Roberts A, Kondaiah P, Tucker A, Madri J. Vascular Cell Responses to TGF-β3 Mimic Those of TGF-β1 in vitro. Growth Factors 1991, 5: 149-158. PMID: 1768438, DOI: 10.3109/08977199109000279.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCell DivisionCell MovementCells, CulturedEndothelium, VascularMuscle, Smooth, VascularTransforming Growth Factor betaVascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro.
Merwin J, Newman W, Beall L, Tucker A, Madri J. Vascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro. American Journal Of Pathology 1991, 138: 37-51. PMID: 1846264, PMCID: PMC1886039.Peer-Reviewed Original ResearchConceptsBovine aortic endothelial cellsBovine aortic smooth muscle cellsGrowth factorGrowth factor beta 1Aortic smooth muscle cellsTGF-beta concentrationsSmooth muscle cellsProliferation of BAECsTGF-beta receptorsBovine aortic endothelial cell migrationType IAortic endothelial cellsTGF beta sCell typesMicrovascular endotheliumVascular cell typesReceptor assayMuscle cellsVascular cellsEndothelial cellsBeta 1Endothelial cell migrationBeta 2Angiogenic assaysBASMCs
1990
Alternative splicing of endothelial cell fibronectin mRNA in the IIICS region. Functional significance.
Kocher O, Kennedy S, Madri J. Alternative splicing of endothelial cell fibronectin mRNA in the IIICS region. Functional significance. American Journal Of Pathology 1990, 137: 1509-24. PMID: 2260635, PMCID: PMC1877716.Peer-Reviewed Original ResearchConceptsAlternative splicingIIICS regionBovine aortic endothelial cellsHuman umbilical vein endothelial cell cDNA libraryRecombinant proteinsEndothelial cell cDNA libraryCell cDNA librarySpecific gene expressionSmooth muscle cellsAortic endothelial cellsEndothelial cellsMuscle cellsB16F10 melanoma cellsMelanoma cellsVascular cell functionCell attachmentFibronectin mRNACDNA clonesCDNA libraryRGDS sequenceGene expressionDNA fragmentsSplicingSequence analysisDifferent cell populations