2014
Stratified control of IGF-I expression by hypoxia and stress hormones in osteoblasts
McCarthy TL, Yun Z, Madri JA, Centrella M. Stratified control of IGF-I expression by hypoxia and stress hormones in osteoblasts. Gene 2014, 539: 141-151. PMID: 24440782, PMCID: PMC4316208, DOI: 10.1016/j.gene.2014.01.011.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsCCAAT-Enhancer-Binding Protein-deltaCell HypoxiaCells, CulturedCore Binding Factor Alpha 1 SubunitDinoprostoneDNADNA ReplicationGene Expression RegulationHydrocortisoneInsulin-Like Growth Factor IOsteoblastsOxygenPromoter Regions, GeneticProtein BiosynthesisRatsRats, Sprague-DawleyRegulatory Elements, TranscriptionalRNA, MessengerConceptsIGF-I expressionStress hormonesIGF-I gene promoterIGF-I mRNAInfluence of PGE2Transcription factor C/EBPδOsteoblast transcription factor Runx2Hypoxic inhibitionI expressionPGE2Stimulatory effectGlucocorticoidsHypoxiaHIF activityHypoxic stressTranscription factor Runx2Bone cellsIGFProlonged exposureHormoneAlkaline phosphatase activitySystemic regulationOsteoblastsResponse elementRunx2
2003
Transcriptional Up-regulation of Endothelial Cell Matrix Metalloproteinase-2 in Response to Extracellular Cues Involves GATA-2*
Han X, Boyd PJ, Colgan S, Madri JA, Haas TL. Transcriptional Up-regulation of Endothelial Cell Matrix Metalloproteinase-2 in Response to Extracellular Cues Involves GATA-2*. Journal Of Biological Chemistry 2003, 278: 47785-47791. PMID: 14512418, DOI: 10.1074/jbc.m309482200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernBlotting, WesternCell NucleusCells, CulturedCollagenCOS CellsDNA-Binding ProteinsEndothelial CellsExtracellular MatrixGATA2 Transcription FactorGelatinGenes, ReporterLuciferasesMatrix Metalloproteinase 2MicrocirculationMolecular Sequence DataNeovascularization, PhysiologicPhenotypePromoter Regions, GeneticProtein BindingRatsTranscription FactorsTranscription, GeneticTranscriptional ActivationTransfectionUp-Regulation
2002
Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpression
1994
Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection.
Fodor W, Williams B, Matis L, Madri J, Rollins S, Knight J, Velander W, Squinto S. Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 11153-11157. PMID: 7526391, PMCID: PMC45185, DOI: 10.1073/pnas.91.23.11153.Peer-Reviewed Original ResearchConceptsHuman complement inhibitorsHyperacute rejectionComplement inhibitorsAnti-porcine antibodiesTerminal complement inhibitorTransgenic pigsXenogeneic transplantation modelPorcine cell typesHigh-level cell surface expressionVariety of murineXenogeneic complementsXenogeneic organ transplantationGraft failureOrgan rejectionVessel occlusionCell surface expressionHyperacute organ rejectionTransplantation modelOrgan transplantationXenogeneic organsVascular endotheliumTransgenic miceSerum complementAutologous complementTransplantation