Aortic endothelial cell proteoheparan sulfate. I. Isolation and characterization of plasmamembrane-associated and extracellular species.
Keller R, Silbert J, Furthmayr H, Madri J. Aortic endothelial cell proteoheparan sulfate. I. Isolation and characterization of plasmamembrane-associated and extracellular species. American Journal Of Pathology 1987, 128: 286-98. PMID: 3039849, PMCID: PMC1899622.Peer-Reviewed Original ResearchConceptsDaltons apparent molecular weightProteoheparan sulfate speciesHeparan sulfate chainsApparent molecular weightHS IBovine aortic endothelial cellsSulfate chainsCultured bovine aortic endothelial cellsCore proteinAortic endothelial cellsCell surface localizationPlasma membrane fractionEndothelial cellsPulse-chase experimentsCsCl density centrifugationThird speciesPlasma membraneSubcellular fractionationExtracellular speciesSpecialized functionsMatrix localizationSurface localizationMembrane fractionSulfate biosynthesisMedium species