2008
Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2002
Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpression
2001
Focal Adhesion Kinase Activates Stat1 in Integrin-mediated Cell Migration and Adhesion*
Xie B, Zhao J, Kitagawa M, Durbin J, Madri J, Guan J, Fu X. Focal Adhesion Kinase Activates Stat1 in Integrin-mediated Cell Migration and Adhesion*. Journal Of Biological Chemistry 2001, 276: 19512-19523. PMID: 11278462, DOI: 10.1074/jbc.m009063200.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCell AdhesionCell LineCell MovementDNA-Binding ProteinsDose-Response Relationship, DrugEnzyme ActivationFibroblastsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene DeletionGlutathione TransferaseHumansIntegrinsMicroscopy, FluorescenceMutagenesis, Site-DirectedMutationPhosphorylationPlasmidsPrecipitin TestsProtein BindingProtein Structure, TertiaryProtein-Tyrosine KinasesSignal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorTime FactorsTrans-ActivatorsTransfectionConceptsFocal adhesion kinaseCell adhesionCell migrationFAK-deficient cellsIntegrin/focal adhesion kinaseC-terminal deletionsAdhesion kinaseTerminal domainFAK localizationTranscription pathwayFocal contactsSignal transducerSTAT1PathwayRecent studiesAdhesionMigrationCellsKinaseIntegrinsSTAT3DeletionActivatorFirst timeActivation
1999
Hyperglycemia-Induced Vasculopathy in the Murine Vitelline Vasculature Correlation with PECAM-1/CD31 Tyrosine Phosphorylation State
Pinter E, Mahooti S, Wang Y, Imhof B, Madri J. Hyperglycemia-Induced Vasculopathy in the Murine Vitelline Vasculature Correlation with PECAM-1/CD31 Tyrosine Phosphorylation State. American Journal Of Pathology 1999, 154: 1367-1379. PMID: 10329590, PMCID: PMC1866605, DOI: 10.1016/s0002-9440(10)65391-6.Peer-Reviewed Original ResearchConceptsPECAM-1 tyrosine phosphorylationTyrosine phosphorylation stateMurine conceptusYolk sacCell-extracellular matrixFirst organ systemCell-factor interactionsTyrosine dephosphorylationEndothelial cell migrationEmbryonic angioblastsEmbryonic developmentMesodermal cellsVasculogenesis/angiogenesisTyrosine phosphorylationPhosphorylation statePerinatal lethalityNormal organogenesisVascular systemCell migrationHematopoietic cellsDiabetic pregnant miceComplex vascular systemVasculogenesisPECAM-1 expressionOffspring
1998
Platelet endothelial cell adhesion molecule-1 expression modulates endothelial cell migration in vitro.
Kim C, Wang T, Madri J. Platelet endothelial cell adhesion molecule-1 expression modulates endothelial cell migration in vitro. Laboratory Investigation 1998, 78: 583-90. PMID: 9605183.Peer-Reviewed Original ResearchConceptsWild-type PECAM-1Endothelial cell migrationEndogenous PECAM-1Cell migrationCell-extracellular matrix interactionsMatrix interactionsTyrosine phosphorylation stateMurine conceptusCell-cell adhesionC-myc epitopeIntegrin-extracellular matrix interactionsCell-cell interactionsPECAM-1Endothelial cellsDynamic dephosphorylationCytoskeletal reorganizationEmbryonic vasculogenesisPhosphorylation stateMigratory stateMigration rateRetroviral transductionCultured endothelial cellsEndothelial cell adhesion molecule-1 expressionEndothelial cell adhesion molecule-1Y686The synergistic activity of αvβ3 integrin and pdgf receptor increases cell migration
Woodard A, García-Cardeña G, Leong M, Madri J, Sessa W, Languino L. The synergistic activity of αvβ3 integrin and pdgf receptor increases cell migration. Journal Of Cell Science 1998, 111: 469-478. PMID: 9443896, DOI: 10.1242/jcs.111.4.469.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntigens, CDBecaplerminCell AdhesionCell DivisionCell MovementCells, CulturedEndothelium, VascularHumansIntegrin alphaVLamininMiceOligopeptidesPlatelet-Derived Growth FactorProto-Oncogene Proteins c-sisRatsReceptor, Platelet-Derived Growth Factor betaReceptors, Platelet-Derived Growth FactorReceptors, VitronectinVitronectinConceptsMicrovascular endothelial cellsGrowth factor receptorGrowth factorEndothelial cellsFactor receptorCell migrationSynergistic activityPlatelet-derived growth factor receptorRat microvascular endothelial cellsAlphavbeta3 integrinGrowth factor-BBNIH 3T3 cellsTissue infiltrationFactor-BBNeovessel formationDifferent antibodiesPlateletsΑvβ3 integrinReceptorsAlphavbeta3Beta1 integrinCell's abilityIntegrinsCellsNovel pathwayThree-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*
Haas T, Davis S, Madri J. Three-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*. Journal Of Biological Chemistry 1998, 273: 3604-3610. PMID: 9452488, DOI: 10.1074/jbc.273.6.3604.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCollagenDrug CombinationsEndothelium, VascularEnzyme ActivationEnzyme InhibitorsGelatinasesLamininMatrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PhysiologicProtein ConformationProteoglycansRatsRats, Sprague-DawleyRNA, MessengerSignal TransductionTissue Inhibitor of Metalloproteinase-2ConceptsMT1-MMPTwo-dimensional cultureEndothelial cellsThree-dimensional collagen matrixEndothelial cell organizationEndothelial cell angiogenesisEndothelial cell migrationCell organizationCoordinate expressionPutative activatorMT1-MMP activationMMP-2Matrix metalloproteinasesCell migrationFunctional roleMatrix interactionsMatrix remodelingCollagen matrixCell angiogenesisEndothelial cell networksMMP-2 proteinProtein
1997
Platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) tyrosine phosphorylation state changes during vasculogenesis in the murine conceptus.
Pinter E, Barreuther M, Lu T, Imhof B, Madri J. Platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) tyrosine phosphorylation state changes during vasculogenesis in the murine conceptus. American Journal Of Pathology 1997, 150: 1523-30. PMID: 9137078, PMCID: PMC1858227.Peer-Reviewed Original ResearchConceptsBlood islandsSrc homology 2 domain-containing proteinsDomain-containing proteinsPhosphorylation state changesPlatelet endothelial cell adhesion molecule-1PECAM-1 cytoplasmic domainMurine conceptusParticular tyrosine residueCell-extracellular matrixDifferential tyrosine phosphorylationCell-cell interactionsEndothelial cell migrationCytoplasmic domainEmbryonic angioblastsMesodermal cellsTyrosine phosphorylationTyrosine residuesCellular localizationCell migrationAngioblastsPhosphorylationGrowth factorVasculogenesisCell adhesion molecule-1MorphogensT cell adhesion to endothelial cells and extracellular matrix is modulated upon transendothelial cell migration.
Romanic A, Graesser D, Baron J, Visintin I, Janeway C, Madri J. T cell adhesion to endothelial cells and extracellular matrix is modulated upon transendothelial cell migration. Laboratory Investigation 1997, 76: 11-23. PMID: 9010446.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCell AdhesionCell MovementCells, CulturedCollagenEncephalomyelitis, Autoimmune, ExperimentalEndothelium, VascularExtracellular MatrixFibronectinsFlow CytometryIntegrin alpha4beta1IntegrinsIntercellular Adhesion Molecule-1Interleukin-2Lymphocyte Function-Associated Antigen-1MiceMice, Inbred StrainsMyelin Basic ProteinPeptide FragmentsReceptors, Lymphocyte HomingReceptors, Very Late AntigenRecombinant ProteinsT-LymphocytesUp-RegulationVascular Cell Adhesion Molecule-1ConceptsAdhesion molecule-1T cellsMolecule-1Recombinant vascular cell adhesion molecule-1Recombinant intercellular adhesion molecule-1Experimental autoimmune encephalomyelitis (EAE) miceLate activation antigen-4Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1T cell migrationExtracellular matrixCell migrationTransendothelial cell migrationAntigen-4Integrin surface expressionInflammatory responseCD4 expressionBrain sectionsT cell adhesionPerivascular tissueEndothelial cellsIntegrin expressionCell-ECM interactionsSurface expression
1996
Role of plasminogen activator inhibitor in the reciprocal regulation of bovine aortic endothelial and smooth muscle cell migration by TGF-beta 1.
Petzelbauer E, Springhorn J, Tucker A, Madri J. Role of plasminogen activator inhibitor in the reciprocal regulation of bovine aortic endothelial and smooth muscle cell migration by TGF-beta 1. American Journal Of Pathology 1996, 149: 923-31. PMID: 8780396, PMCID: PMC1865168.Peer-Reviewed Original ResearchConceptsVascular cell typesCell typesSmooth muscle cellsPlasminogen activator inhibitorMuscle cellsAnti-catalytic antibodyActivator inhibitorExtracellular matrix componentsMigratory rateVascular smooth muscle cellsEndothelial cellsSmooth muscle cell migrationUrokinase plasminogen activatorMigratory behaviorReciprocal regulationMuscle cell migrationStable transfectantsCell migrationExtracellular matrixIntegrin synthesisMatrix componentsMigratory responseProteolytic phenotypeProteolytic activityGrowth factor
1995
Modulation of cell spreading and migration by pp125FAK phosphorylation.
Sankar S, Mahooti-Brooks N, Hu G, Madri J. Modulation of cell spreading and migration by pp125FAK phosphorylation. American Journal Of Pathology 1995, 147: 601-8. PMID: 7677174, PMCID: PMC1870973.Peer-Reviewed Original ResearchConceptsPp125FAK tyrosine phosphorylationTyrosine phosphorylationCell migrationCell spreadingIntegrin-mediated signalingFocal adhesion kinaseKinase-negative mutantPp125FAK phosphorylationAdhesion kinaseMigration ratePhosphorylationCascade pathwayFaster migration rateControl cellsSlower migration rateType I collagenEnhanced levelsCellsI collagenFibronectinMigrationPp125FAKMutantsKinaseSignaling
1994
Effect of tyrosine kinase inhibition on basal and epidermal growth factor‐stimulated human Caco‐2 enterocyte sheet migration and proliferation
Basson M, Turowski G, Zarif A, Modlin I, Beidler D, Jena B, Madri J. Effect of tyrosine kinase inhibition on basal and epidermal growth factor‐stimulated human Caco‐2 enterocyte sheet migration and proliferation. Journal Of Cellular Physiology 1994, 160: 491-501. PMID: 8077287, DOI: 10.1002/jcp.1041600312.Peer-Reviewed Original ResearchConceptsEpidermal growth factorTyrosine kinaseTyrosine kinase regulationEGF-stimulated migrationProtein-linked DNA breaksSubstrate-binding siteTyrosine kinase inhibitor genisteinCell-matrix interactionsDNA topoisomerase activityKinase inhibitor genisteinKinase regulationATP bindingMonolayer expansionEGF stimulationSheet migrationSubunit organizationDNA breaksTopoisomerase activityEGF receptorInhibitor genisteinCell migrationCell proliferationKinase inhibitionTyrosine kinase inhibitionKinaseExtracellular Matrix‐Degrading Proteinases in the Nervous System
Romanic A, Madri J. Extracellular Matrix‐Degrading Proteinases in the Nervous System. Brain Pathology 1994, 4: 145-156. PMID: 8061860, DOI: 10.1111/j.1750-3639.1994.tb00825.x.Peer-Reviewed Original ResearchConceptsCell-ECM interactionsExtracellular matrixMatrix-degrading proteinasesNeuronal cell migrationExtracellular matrix-degrading proteinasesCell migrationNervous systemECM degradationNeurite outgrowthCell proliferationDrug designProteolytic activityNew insightsPathological conditionsBrain tumor growthTumor growthMatrix metalloproteinasesProteinasesForm of therapyCentral nervous systemInhibitorsPlasminogen activatorTraffickingLeukocyte traffickingNerve demyelination
1993
Fibronectin expression correlates with U937 cell adhesion to migrating bovine aortic endothelial cells in vitro.
Hauser I, Setter E, Bell L, Madri J. Fibronectin expression correlates with U937 cell adhesion to migrating bovine aortic endothelial cells in vitro. American Journal Of Pathology 1993, 143: 173-80. PMID: 7686342, PMCID: PMC1886955.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell AdhesionCell MovementCells, CulturedDrug ResistanceEndothelium, VascularFibronectinsFluorescent Antibody TechniqueHumansIntegrinsLeukemia, Promyelocytic, AcuteNeomycinProto-Oncogene MasProto-Oncogene Proteins pp60(c-src)Transforming Growth Factor betaTumor Cells, CulturedConceptsCell adhesionU937 cell adhesionEndothelial cell populationSRC proto-oncogeneEndothelial cellsCell populationsExtracellular matrix componentsEndothelial cell migrationBovine aortic endothelial cellsExtracellular matrix synthesisDenudation injuryFibronectin depositionCell migrationProto-oncogeneVascular endothelial cellsU937 cellsAortic endothelial cellsAmount of fibronectinMatrix componentsMigratory responseEndothelial cell monolayersMatrix synthesisCellsCell monolayersAdhesionPhotoinhibition of smooth muscle cell migration: Potential therapy for restenosis
Deckelbaum L, Scott J, Stetz M, O'Brien K, Sumpio B, Madri J, Bell L. Photoinhibition of smooth muscle cell migration: Potential therapy for restenosis. Lasers In Surgery And Medicine 1993, 13: 4-11. PMID: 8426525, DOI: 10.1002/lsm.1900130104.Peer-Reviewed Original ResearchConceptsSmooth muscle cell migrationMuscle cell migrationLow energy light irradiationNeointima formationBroadband white lightCell migrationLight irradiationIncidence of restenosisLuminal cross-sectional areaSmooth muscle cell monolayersSmooth muscle cellsFibrointimal thickeningArterial injuryPotential therapyDaily radiant exposureRadiant exposureMuscle cellsThymidine incorporationRestenosisCross-sectional areaMigration of cellsCell proliferationCell monolayersMigration kineticsWhite light
1992
Platelet endothelial cell adhesion molecule, PECAM‐1, modulates cell migration
Schimmenti L, Yan H, Madri J, Albelda S. Platelet endothelial cell adhesion molecule, PECAM‐1, modulates cell migration. Journal Of Cellular Physiology 1992, 153: 417-428. PMID: 1429859, DOI: 10.1002/jcp.1041530222.Peer-Reviewed Original ResearchConceptsCell-cell adhesionCell-cell adhesion moleculesCell-cell contactPlatelet endothelial cell adhesion molecule-1Cell migrationIntegral membrane proteinsAdhesion moleculesPECAM-1 moleculesControl cellsCell surface adhesion moleculesModulates cell migrationNeomycin resistance geneEndothelial cell migrationMembrane proteinsCell adhesion moleculeEndothelial cellsExtracellular domainSurface adhesion moleculesNull cellsNIH/3T3 cellsResistance genesExtracellular matrixTransfectantsAdjacent cellsMigration assaysModulation of vascular cell behavior by transforming growth factors β
Madri J, Bell L, Merwin J. Modulation of vascular cell behavior by transforming growth factors β. Molecular Reproduction And Development 1992, 32: 121-126. PMID: 1637550, DOI: 10.1002/mrd.1080320207.Peer-Reviewed Original ResearchConceptsVascular cell typesCell typesCellular responsesCell surfaceTGF-beta receptorsVascular cell behaviorGrowth factor βDistinct bioassaysDifferent isoformsCell migrationCell behaviorInhibition of proliferationVascular cell responsesSmooth muscle cellsUnique binding profileAngiogenic assaysIsoformsBinding profileCell populationsBovine aortic endothelialFactor βMuscle cellsType IBASMCsAortic endothelialAutocrine angiotensin system regulation of bovine aortic endothelial cell migration and plasminogen activator involves modulation of proto-oncogene pp60c-src expression.
Bell L, Luthringer D, Madri J, Warren S. Autocrine angiotensin system regulation of bovine aortic endothelial cell migration and plasminogen activator involves modulation of proto-oncogene pp60c-src expression. Journal Of Clinical Investigation 1992, 89: 315-320. PMID: 1370299, PMCID: PMC442850, DOI: 10.1172/jci115578.Peer-Reviewed Original ResearchMeSH Keywords1-Sarcosine-8-Isoleucine Angiotensin IIAngiotensin IIAngiotensin Receptor AntagonistsAngiotensin-Converting Enzyme InhibitorsAnimalsAortaCattleCell MovementCells, CulturedEnalaprilEndothelium, VascularGene Expression Regulation, ViralLisinoprilPlasminogen ActivatorsProtein KinasesProto-Oncogene Proteins pp60(c-src)Receptors, AngiotensinRNA, MessengerConceptsEndothelial cell migrationC-Src expressionCell migrationC-SrcProto-oncogene c-srcC-Src kinase activityPp60c-src expressionCoding sequenceKinase activityU-PABovine aortic endothelial cell migrationAngiotensin-converting enzyme inhibitor lisinoprilRetroviral vectorsProtein 3Plasminogen activatorStable infectionExpressionDenudation injuryPlasminogen activator activityActivatorActivity 2Cell expressionAngiotensin system inhibitionActivator activityEndothelial cell expression
1990
Influence of the angiotensin system on endothelial and smooth muscle cell migration.
Bell L, Madri J. Influence of the angiotensin system on endothelial and smooth muscle cell migration. American Journal Of Pathology 1990, 137: 7-12. PMID: 2164777, PMCID: PMC1877705.Peer-Reviewed Original ResearchConceptsSmooth muscle cell migrationVessel wall responseMuscle cell migrationBovine aortic endothelial cellsAortic smooth muscle cell migrationMedial smooth muscle cellsEndothelial cellsAngiotensin system componentsIle8-angiotensin IIEnzyme inhibitor lisinoprilSmooth muscle cellsCell migrationAortic endothelial cellsAngiotensin systemAngiotensin-converting enzyme inhibitor lisinoprilInhibitor lisinoprilLuminal sizeVessel functionMuscle cellsCell monolayersInjuryCultured cell monolayersPresent studyImportant determinantCells
1988
Extracellular matrix specificity for the differentiation of capillary endothelial cells
Carley W, Milici A, Madri J. Extracellular matrix specificity for the differentiation of capillary endothelial cells. Experimental Cell Research 1988, 178: 426-434. PMID: 3049122, DOI: 10.1016/0014-4827(88)90411-9.Peer-Reviewed Original ResearchConceptsExtracellular matrixCapillary endothelial cellsEndothelial cellsSpecific extracellular matrixEndothelial cell migrationVivo phenotypeDifferentiated phenotypeCell migrationCell typesCell growthCell dedifferentiationEndothelial cell growthMatrix componentsMembrane structurePhenotypeCellsExpressionCulture methodCytoplasmProteinGrowthMadinDifferentiationDedifferentiationGreater number