2014
NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia*
Lee C, Liu A, Miranda-Ribera A, Hyun SW, Lillehoj EP, Cross AS, Passaniti A, Grimm PR, Kim BY, Welling PA, Madri JA, DeLisser HM, Goldblum SE. NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia*. Journal Of Biological Chemistry 2014, 289: 9121-9135. PMID: 24550400, PMCID: PMC3979388, DOI: 10.1074/jbc.m114.555888.Peer-Reviewed Original ResearchConceptsHuman pulmonary microvascular ECsCapillary-like tube formationEC tube formationTube formationCell adhesion molecule-1Pulmonary microvascular ECsHuman Lung Microvascular EndotheliaNeu1 sialidaseLung microvascular endotheliumAdhesion molecule-1Endothelial cell adhesion molecule-1Platelet endothelial cell adhesion molecule-1Endothelial cell expressionMultiplicity of infectionMicrovascular endotheliumMolecule-1Microvascular ECsCell expressionCD31Matrigel substrateSialylation statePeanut agglutinin lectinAdhesion moleculesInhibitory effectAngiogenic phenotype
2006
PECAM‐1 modulates thrombin‐induced tissue factor expression on endothelial cells
Zhang JJ, Kelm RJ, Biswas P, Kashgarian M, Madri JA. PECAM‐1 modulates thrombin‐induced tissue factor expression on endothelial cells. Journal Of Cellular Physiology 2006, 210: 527-537. PMID: 17111362, DOI: 10.1002/jcp.20908.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsApoptosisBlood CoagulationCells, CulturedDisease Models, AnimalDown-RegulationEarly Growth Response Protein 1Endothelial CellsFibrinHumansKidneyMaleMAP Kinase Signaling SystemMiceMice, Inbred C57BLMice, KnockoutOligodeoxyribonucleotides, AntisensePlatelet Endothelial Cell Adhesion Molecule-1Receptor, PAR-1Reperfusion InjuryRNA, MessengerThrombinThromboplastinThrombosisConceptsTissue factor expressionHuman umbilical vein endothelial cellsFactor expressionPECAM-1TF inductionEndothelial cellsP38 phosphorylationCell adhesion molecule-1Transient renal ischemiaThrombin receptor PAR-1PAR-1 antagonistsPertussis toxin inhibitionAdhesion molecule-1Endothelial cell adhesion molecule-1Receptor PAR-1PI3K-Akt phosphorylationGalphai/o subunitsPECAM-1 expressionRho-kinase activityUmbilical vein endothelial cellsVein endothelial cellsRenal ischemiaEgr-1 expressionFibrin depositionPlatelet functionPECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation
Biswas P, Canosa S, Schoenfeld D, Schoenfeld J, Li P, Cheas LC, Zhang J, Cordova A, Sumpio B, Madri JA. PECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation. American Journal Of Pathology 2006, 169: 314-324. PMID: 16816383, PMCID: PMC1698776, DOI: 10.2353/ajpath.2006.051112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBlotting, WesternCapillary PermeabilityCells, CulturedEndothelial CellsFluorescent Antibody TechniqueGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHistamineHistamine AgentsHumansMiceModels, BiologicalPhosphatidylinositol 3-KinasesPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Proto-Oncogene Proteins c-aktReceptors, HistamineSignal TransductionConceptsAdherens junctionsSerine phosphorylationSrc homology 2 domainBeta-catenin expression levelsAdherens junction componentsSerine phosphorylation levelEndothelial cellsΒ-catenin phosphorylationPECAM-1Cell biological responsesCytoplasmic domainSHP-2Proteosomal degradationGSK-3betaDynamic regulatorJunction componentsPhosphorylation levelsPhosphorylationEndothelial cell adhesion molecule-1Expression levelsGSK-3βBiological responsesEndothelial barrier permeabilityMice exhibitCell adhesion molecule-1
2005
Enhanced Susceptibility to Endotoxic Shock and Impaired STAT3 Signaling in CD31-Deficient Mice
Carrithers M, Tandon S, Canosa S, Michaud M, Graesser D, Madri JA. Enhanced Susceptibility to Endotoxic Shock and Impaired STAT3 Signaling in CD31-Deficient Mice. American Journal Of Pathology 2005, 166: 185-196. PMID: 15632011, PMCID: PMC1602311, DOI: 10.1016/s0002-9440(10)62243-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDisease SusceptibilityDNA-Binding ProteinsEndothelium, VascularFemaleFlow CytometryGene Expression RegulationLipopolysaccharidesMiceMice, Inbred C57BLMice, KnockoutPlatelet Endothelial Cell Adhesion Molecule-1Pulmonary CirculationShock, SepticSpleenSTAT3 Transcription FactorTrans-ActivatorsTumor Necrosis Factor-alphaVanadatesConceptsCD31-deficient miceAcute phase responseSeptic shockEndothelial integritySerum tumor necrosis factor alphaTumor necrosis factor alphaEndothelial cellsCell adhesion molecule-1Necrosis factor alphaAdhesion molecule-1Endothelial cell adhesion molecule-1Wild-type controlsIL-6Endotoxic shockMCP-1Neutrophil transmigrationPhase responseMCP-5Factor alphaImmune stimuliVascular permeabilityInterferon gammaKnockout miceMolecule-1STAT3 Signaling
2003
Abolition of arteriolar dilation but not constriction to histamine in cremaster muscle of eNOS–/– mice
Payne GW, Madri JA, Sessa WC, Segal SS. Abolition of arteriolar dilation but not constriction to histamine in cremaster muscle of eNOS–/– mice. AJP Heart And Circulatory Physiology 2003, 285: h493-h498. PMID: 12689855, DOI: 10.1152/ajpheart.00071.2003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriolesCimetidineEndothelium, VascularHistamineHistamine H1 AntagonistsHistamine H2 AntagonistsMaleMiceMice, Inbred C57BLMice, Inbred StrainsMuscle, SkeletalNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIPlatelet Endothelial Cell Adhesion Molecule-1PyrilamineReceptors, HistamineSignal TransductionVasoconstrictionVasodilationConceptsENOS-/- miceMuscle blood flowVasomotor responsesBlood flowCremaster muscleCell adhesion molecule-1Anesthetized C57Bl6 miceBiphasic vasomotor responseSecond-order arteriolesAdhesion molecule-1Endothelial cell adhesion molecule-1Platelet endothelial cell adhesion molecule-1Nitric oxide releaseTopical histamineConstrictor responsesArteriolar dilationNomega-nitroC57BL6 miceH1 receptorsPharmacological interventionsPermeability of capillariesSmooth muscleMicrovascular endotheliumTissue perfusionCumulative additionLack of Platelet Endothelial Cell Adhesion Molecule-1 Attenuates Foreign Body Inflammation because of Decreased Angiogenesis
Solowiej A, Biswas P, Graesser D, Madri JA. Lack of Platelet Endothelial Cell Adhesion Molecule-1 Attenuates Foreign Body Inflammation because of Decreased Angiogenesis. American Journal Of Pathology 2003, 162: 953-962. PMID: 12598328, PMCID: PMC1868115, DOI: 10.1016/s0002-9440(10)63890-4.Peer-Reviewed Original ResearchConceptsCell adhesion molecule-1Adhesion molecule-1Endothelial cell adhesion molecule-1Foreign body inflammationBody inflammationMolecule-1Knockout animalsAcute inflammatory modelForeign body implantsAntibody-blocking studiesPECAM-1 knockout micePlatelet endothelial cell adhesion molecule-1PECAM-1 resultsDiminished deliveryNeutrophil accumulationNeutrophil infiltrationLeukocyte accumulationInflammatory modelChronic processDecreased angiogenesisCD31 expressionKnockout miceMice exhibitEndothelial cellsLeukocyte transmigrationPlatelet–endothelial cell adhesion molecule-1 modulates endothelial migration through its immunoreceptor tyrosine-based inhibitory motif
Gratzinger D, Barreuther M, Madri JA. Platelet–endothelial cell adhesion molecule-1 modulates endothelial migration through its immunoreceptor tyrosine-based inhibitory motif. Biochemical And Biophysical Research Communications 2003, 301: 243-249. PMID: 12535670, DOI: 10.1016/s0006-291x(02)02982-0.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAmino Acid MotifsAnimalsCattleCell MovementCells, CulturedEndothelium, VascularEnzyme ActivationIntracellular Signaling Peptides and ProteinsMiceMice, KnockoutPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein BindingProtein Tyrosine Phosphatase, Non-Receptor Type 11Protein Tyrosine PhosphatasesRecombinant Fusion ProteinsTyrosineConceptsSHP-2Tyrosine phosphatase SHP-2Endothelial migrationFocal contact componentsPlatelet endothelial cell adhesion molecule-1Phosphatase SHP-2Cell-cell junctionsImmunoreceptor tyrosine-based inhibitory motifCell-substrate adhesionFocal adhesion kinaseTyrosine-based inhibitory motifPECAM-1Endothelial cellsPECAM-1 phosphorylationSelective dephosphorylationAdhesion kinaseTyrosine phosphorylationAdhesion proteinsRecombinant proteinsCytoskeletal fractionCell adhesion molecule-1Coordinated migrationInhibitory motifPhosphorylationAdhesion molecule-1
2002
Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice
Graesser D, Solowiej A, Bruckner M, Osterweil E, Juedes A, Davis S, Ruddle NH, Engelhardt B, Madri JA. Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice. Journal Of Clinical Investigation 2002, 109: 383-392. PMID: 11827998, PMCID: PMC150854, DOI: 10.1172/jci13595.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisPECAM-1-deficient miceEndothelial cellsAutoimmune encephalomyelitisVascular permeabilityDevelopment of EAET lymphocyte transendothelial migrationEarly onsetHuman autoimmune disease multiple sclerosisAutoimmune disease multiple sclerosisCell adhesion molecule-1Altered vascular permeabilityCNS vascular permeabilityMononuclear cell extravasationDisease multiple sclerosisPlatelet/endothelial cell adhesion molecule-1Wild-type miceAdhesion molecule-1Endothelial cell adhesion molecule-1Subsets of leukocytesPECAM-1 expressionLymphocyte transendothelial migrationEarly time pointsHistamine challengeMultiple sclerosis
1998
The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis.
Graesser D, Mahooti S, Haas T, Davis S, Clark R, Madri J. The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis. Laboratory Investigation 1998, 78: 1445-58. PMID: 9840619.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDCell AdhesionCell MembraneCell MovementCells, CulturedClone CellsEncephalomyelitis, Autoimmune, ExperimentalEndothelium, VascularGelatinasesIntegrin alpha4Matrix Metalloproteinase 14Matrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMice, Inbred StrainsProtease InhibitorsTissue Inhibitor of Metalloproteinase-2T-LymphocytesTransfectionConceptsExperimental autoimmune encephalomyelitisT cell clonesRecombinant vascular cell adhesion molecule-1Autoreactive T cell clonesAlpha4 integrinsMMP-2EAE inductionAutoimmune encephalomyelitisMyelin basic protein-reactive T cell clonesDisease processMatrix metalloproteinaseVascular cell adhesion molecule-1Cell adhesion molecule-1Human multiple sclerosisSusceptible mouse strainsAdhesion molecule-1T cell transmigrationMatrix metalloproteinase-2Microvascular endothelial cellsAlpha4 integrin expressionExpression of alpha4Adoptive transferMultiple sclerosisMouse modelEndothelial cell layer
1997
Platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) tyrosine phosphorylation state changes during vasculogenesis in the murine conceptus.
Pinter E, Barreuther M, Lu T, Imhof B, Madri J. Platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) tyrosine phosphorylation state changes during vasculogenesis in the murine conceptus. American Journal Of Pathology 1997, 150: 1523-30. PMID: 9137078, PMCID: PMC1858227.Peer-Reviewed Original ResearchConceptsBlood islandsSrc homology 2 domain-containing proteinsDomain-containing proteinsPhosphorylation state changesPlatelet endothelial cell adhesion molecule-1PECAM-1 cytoplasmic domainMurine conceptusParticular tyrosine residueCell-extracellular matrixDifferential tyrosine phosphorylationCell-cell interactionsEndothelial cell migrationCytoplasmic domainEmbryonic angioblastsMesodermal cellsTyrosine phosphorylationTyrosine residuesCellular localizationCell migrationAngioblastsPhosphorylationGrowth factorVasculogenesisCell adhesion molecule-1MorphogensT cell adhesion to endothelial cells and extracellular matrix is modulated upon transendothelial cell migration.
Romanic A, Graesser D, Baron J, Visintin I, Janeway C, Madri J. T cell adhesion to endothelial cells and extracellular matrix is modulated upon transendothelial cell migration. Laboratory Investigation 1997, 76: 11-23. PMID: 9010446.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCell AdhesionCell MovementCells, CulturedCollagenEncephalomyelitis, Autoimmune, ExperimentalEndothelium, VascularExtracellular MatrixFibronectinsFlow CytometryIntegrin alpha4beta1IntegrinsIntercellular Adhesion Molecule-1Interleukin-2Lymphocyte Function-Associated Antigen-1MiceMice, Inbred StrainsMyelin Basic ProteinPeptide FragmentsReceptors, Lymphocyte HomingReceptors, Very Late AntigenRecombinant ProteinsT-LymphocytesUp-RegulationVascular Cell Adhesion Molecule-1ConceptsAdhesion molecule-1T cellsMolecule-1Recombinant vascular cell adhesion molecule-1Recombinant intercellular adhesion molecule-1Experimental autoimmune encephalomyelitis (EAE) miceLate activation antigen-4Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1T cell migrationExtracellular matrixCell migrationTransendothelial cell migrationAntigen-4Integrin surface expressionInflammatory responseCD4 expressionBrain sectionsT cell adhesionPerivascular tissueEndothelial cellsIntegrin expressionCell-ECM interactionsSurface expression
1994
The induction of 72-kD gelatinase in T cells upon adhesion to endothelial cells is VCAM-1 dependent.
Romanic A, Madri J. The induction of 72-kD gelatinase in T cells upon adhesion to endothelial cells is VCAM-1 dependent. Journal Of Cell Biology 1994, 125: 1165-1178. PMID: 7515069, PMCID: PMC2120055, DOI: 10.1083/jcb.125.5.1165.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Recombinant VCAM-1T cellsT cell transmigrationEndothelial cellsNegative endothelial cellsCell transmigrationCell adhesion molecule-1Transmigrated T cellsExperimental autoimmune encephalomyelitisT cell extravasationAdhesion molecule-1Late antigen-4VCAM-1 positive cellsMyelin basic proteinAutoimmune encephalomyelitisEndothelial cell monolayersAntigen-4Brain parenchymaNormal miceEndothelial cell layerPositive cellsMolecule-1Perivascular tissueT cell adhesion
1993
Surface expression of alpha 4 integrin by CD4 T cells is required for their entry into brain parenchyma.
Baron JL, Madri JA, Ruddle NH, Hashim G, Janeway CA. Surface expression of alpha 4 integrin by CD4 T cells is required for their entry into brain parenchyma. Journal Of Experimental Medicine 1993, 177: 57-68. PMID: 7678116, PMCID: PMC2190872, DOI: 10.1084/jem.177.1.57.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalBrainCD4-Positive T-LymphocytesCell Adhesion MoleculesCell MovementClone CellsEncephalomyelitis, Autoimmune, ExperimentalIntercellular Adhesion Molecule-1MiceMice, Inbred BALB CMyelin Basic ProteinReceptors, Very Late AntigenVascular Cell Adhesion Molecule-1ConceptsAlpha 4 integrinsExperimental autoimmune encephalomyelitisAdhesion molecule-1T cellsBrain parenchymaT cell linesEncephalitogenic clonesAlpha 4Surface expressionMolecule-1Recombinant vascular cell adhesion molecule-1T helper type 1 clonesCD4 T cell linesCloned T cell linesTh1 cytokine interleukin-2Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Tumor necrosis factor betaTh1 T cellsEffector T cellsCD4 T cellsMajor pathogenic factorT cell entryNecrosis factor beta