An in vitro three-dimensional coculture model of cerebral microvascular angiogenesis and differentiation
Ment L, Stewart W, Scaramuzzino D, Madri J. An in vitro three-dimensional coculture model of cerebral microvascular angiogenesis and differentiation. In Vitro Cellular & Developmental Biology - Animal 1997, 33: 684-691. PMID: 9358284, DOI: 10.1007/s11626-997-0126-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAstrocytesBrainCell DifferentiationCoculture TechniquesDogsEndothelium, VascularEnzyme ActivationFibronectinsImmunohistochemistryLamininMicrocirculationMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalNeovascularization, PhysiologicRatsRNA, MessengerUrokinase-Type Plasminogen ActivatorConceptsAstrocyte coculturesThree-dimensional cocultureBrain microvascular endothelial cellsNewborn beagle pupsPostnatal day 1Microvascular endothelial cellsNeonatal rat forebrainCell typesPlasminogen activator activityPreterm birthMicrovascular responsesBeagle pupsThree-dimensional coculture modelDay 1Rat forebrainGlial processesEndothelial proliferationMicrovascular angiogenesisEndothelial cellsCoculture modelPlasminogen zymographyOnly low levelsExtracellular matrix componentsTube formationCoculture