2023
Shared genetic architecture of blood eosinophil counts and asthma in UK Biobank
Li B, Wang Y, Wang Z, Li X, Kay S, Chupp G, Zhao H, Gomez J. Shared genetic architecture of blood eosinophil counts and asthma in UK Biobank. ERJ Open Research 2023, 9: 00291-2023. PMID: 37650091, PMCID: PMC10463033, DOI: 10.1183/23120541.00291-2023.Peer-Reviewed Original ResearchGenome-wide association studiesGenetic architectureGenetic correlation analysisUK BiobankGWAS resultsTranscription factorsInterleukin-4 SignalingBlood eosinophil countsAssociation studiesDoctor-diagnosed asthmaSignificant variantsEosinophil countEuropean ancestryTraitsPathwayGenetic linkType 2 immune responsesType 2 inflammationSignalingCritical associationImmune responseHeterogeneous diseaseTAGCSevere asthmaTherapeutic interventions
2021
Single-cell characterization of a model of poly I:C-stimulated peripheral blood mononuclear cells in severe asthma
Chen A, Diaz-Soto MP, Sanmamed MF, Adams T, Schupp JC, Gupta A, Britto C, Sauler M, Yan X, Liu Q, Nino G, Cruz CSD, Chupp GL, Gomez JL. Single-cell characterization of a model of poly I:C-stimulated peripheral blood mononuclear cells in severe asthma. Respiratory Research 2021, 22: 122. PMID: 33902571, PMCID: PMC8074196, DOI: 10.1186/s12931-021-01709-9.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsSevere asthmaEffector T cellsBlood mononuclear cellsT cellsHealthy controlsPoly IDendritic cellsMononuclear cellsUnstimulated peripheral blood mononuclear cellsInterferon responseTLR3 agonist poly IImpaired interferon responseMain cell subsetsNatural killer cellsPro-inflammatory profilePro-inflammatory pathwaysC stimulationCyTOF profilingHigh CD8Cell typesEffector cellsKiller cellsCell subsetsMain cell types
2016
Noninvasive Analysis of the Sputum Transcriptome Discriminates Clinical Phenotypes of Asthma
Yan X, Chu JH, Gomez J, Koenigs M, Holm C, He X, Perez MF, Zhao H, Mane S, Martinez FD, Ober C, Nicolae DL, Barnes KC, London SJ, Gilliland F, Weiss ST, Raby BA, Cohn L, Chupp GL. Noninvasive Analysis of the Sputum Transcriptome Discriminates Clinical Phenotypes of Asthma. Annals Of The American Thoracic Society 2016, 13 Suppl 1: s104-5. PMID: 27027945, PMCID: PMC5015745, DOI: 10.1513/annalsats.201510-681mg.Peer-Reviewed Original ResearchNitric oxide levelsOxide levelsHigher bronchodilator responseHistory of intubationNormal lung functionClusters of patientsCommon transcriptomic signaturesHigh ratePrebronchodilator FEV1Severe asthmaSteroid requirementsInflammatory characteristicsLung functionBronchodilator responseControl subjectsAsthmaHeterogeneous diseaseClinical phenotypeGene signaturePhysiologic characteristicsWhole bloodTranscriptomic signaturesCluster 1DiseaseHospitalization
2015
Genetic variation in chitinase 3-like 1 (CHI3L1) contributes to asthma severity and airway expression of YKL-40
Gomez JL, Crisafi GM, Holm CT, Meyers DA, Hawkins GA, Bleecker ER, Jarjour N, Investigators S, Cohn L, Chupp GL. Genetic variation in chitinase 3-like 1 (CHI3L1) contributes to asthma severity and airway expression of YKL-40. Journal Of Allergy And Clinical Immunology 2015, 136: 51-58.e10. PMID: 25592985, PMCID: PMC4494869, DOI: 10.1016/j.jaci.2014.11.027.Peer-Reviewed Original ResearchConceptsYKL-40 levelsYKL-40 expressionSerum YKL-40 levelsAsthma severityYKL-40Airway expressionAirway diseaseLung functionSingle nucleotide polymorphismsChitinase 3Severe Asthma Research ProgramHigh YKL-40 expressionOnly single nucleotide polymorphismRisk allele APostbronchodilator FEV1Airflow obstructionFEV1 percentSevere asthmaAsthmatic subjectsAsthma prevalenceHaplotype analysisGene polymorphismsG alleleCHI3L1 geneAsthma