2004
Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences
McKinney JS, Zhang H, Kubori T, Galán J, Altman S. Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences. Nucleic Acids Research 2004, 32: 848-854. PMID: 14762212, PMCID: PMC373343, DOI: 10.1093/nar/gkh219.Peer-Reviewed Original Research
2001
Common and Contrasting Themes of Plant and Animal Diseases
Staskawicz B, Mudgett M, Dangl J, Galan J. Common and Contrasting Themes of Plant and Animal Diseases. Science 2001, 292: 2285-2289. PMID: 11423652, DOI: 10.1126/science.1062013.Peer-Reviewed Original ResearchConceptsHost-pathogen interactionsStudy of plantsDefense signalingHost defense responsesAnimal diseasesDefense responsesEffector proteinsAnimal pathogenesisBacterial pathogenesisPathogen virulencePathogen recognitionMolecular eventsHost resistancePlantsRecent studiesBacterial colonizationSignalingVirulenceProteinColonizationHostMechanismFuture studiesPathogenesisCharacterization of the mutS-proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes
Pancetti A, Galán J. Characterization of the mutS-proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes. FEMS Microbiology Letters 2001, 197: 203-208. PMID: 11313135, DOI: 10.1111/j.1574-6968.2001.tb10604.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChromosome MappingGenes, BacterialGenotypeMiceMice, Inbred BALB CMutationSalmonella typhimuriumVirulenceConceptsPathogenicity islandSecretion systemType III protein secretion systemSPI-1Type III secretion systemHigher eukaryotic hostsProtein secretion systemSet of genesIron uptake systemExpression of genesSegment of DNAPotential pathogenicity islandsEukaryotic hostsFhlA geneGene blockCentisome 63Pig genesFunctional copyTranscription factorsSalmonella chromosomeUnknown functionUptake systemGenesBacterial pathogensHomologGenetic Analysis of Assembly of theSalmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex
Sukhan A, Kubori T, Wilson J, Galán J. Genetic Analysis of Assembly of theSalmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex. Journal Of Bacteriology 2001, 183: 1159-1167. PMID: 11157927, PMCID: PMC94988, DOI: 10.1128/jb.183.4.1159-1167.2001.Peer-Reviewed Original Research
2000
The Flagellar Sigma Factor FliA (ς28) Regulates the Expression of Salmonella Genes Associated with the Centisome 63 Type III Secretion System
Eichelberg K, Galán J. The Flagellar Sigma Factor FliA (ς28) Regulates the Expression of Salmonella Genes Associated with the Centisome 63 Type III Secretion System. Infection And Immunity 2000, 68: 2735-2743. PMID: 10768967, PMCID: PMC97482, DOI: 10.1128/iai.68.5.2735-2743.2000.Peer-Reviewed Original ResearchConceptsType III secretion systemSecretion systemExpression of componentsRegulatory proteinsType III protein secretion systemRegulatory mechanismsSerovar TyphimuriumFlagellar sigma factor FliASigma factor FliAProtein secretion systemTranscriptional regulatory proteinsSalmonella pathogenicity island 1Complex regulatory mechanismsFlagellar regulatory genesPathogenicity island 1Macrophage cell deathEffects of mutationsType III systemCultured epithelial cellsFlagellar genesEnterica serovar TyphimuriumMild centrifugal forceRegulatory genesSalmonella genesS. enterica serovar Typhimurium
1999
Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1
Eichelberg K, Hardt W, Galán J. Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1. Molecular Microbiology 1999, 33: 139-152. PMID: 10411731, DOI: 10.1046/j.1365-2958.1999.01458.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAraC Transcription FactorBacterial ProteinsBiological TransportCells, CulturedChromosome MappingChromosomes, BacterialConsensus SequenceDNA-Binding ProteinsEpithelial CellsGene Expression Regulation, BacterialGenes, BacterialGenes, ReporterHelix-Loop-Helix MotifsMolecular Sequence DataMultigene FamilyPhylogenyRecombinant Fusion ProteinsRepressor ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticVirulenceConceptsType III secretion systemTranscriptional regulatory proteinsPathogenicity island 1Secretion systemRegulatory proteinsType III protein secretion systemSPI-1Island 1Host cellsProtein secretion systemTranscriptional regulatory cascadeSpecific transcriptional regulatory proteinsAdditional regulatory proteinsSignificant sequence similarityAraC-like transcriptional regulatorActin cytoskeletal rearrangementRegulation of expressionInvasion-associated genesExpression of genesNon-phagocytic cellsCentisome 63Effector proteinsTranscriptional regulatorsRegulatory cascadeSequence similarityType III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells
Galán J, Collmer A. Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells. Science 1999, 284: 1322-1328. PMID: 10334981, DOI: 10.1126/science.284.5418.1322.Peer-Reviewed Original ResearchConceptsSecretion systemComplex protein secretion systemsHost cellsType III secretion systemBacterial effector proteinsProtein secretion systemAnimal pathogenic bacteriaHost cellular functionsGram-negative pathogenic bacteriaBacterial devicesPathogenic bacteriaEffector proteinsCellular functionsFlagellar apparatusBacterial speciesEffector moleculesProtein deliveryBacteriaCellsNovel preventionBiologySpeciesPlantsProteinTherapeutic approaches
1998
‘Avirulence genes’ in animal pathogens?
Galán J. ‘Avirulence genes’ in animal pathogens? Trends In Microbiology 1998, 6: 3-6. PMID: 9481815, DOI: 10.1016/s0966-842x(97)01183-9.Peer-Reviewed Original Research
1994
The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins
Kaniga K, Bossio J, Galán J. The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins. Molecular Microbiology 1994, 13: 555-568. PMID: 7997169, DOI: 10.1111/j.1365-2958.1994.tb00450.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAraC Transcription FactorBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceCells, CulturedCloning, MolecularDNA-Binding ProteinsGene Expression Regulation, BacterialGenes, BacterialMembrane ProteinsMembrane Transport ProteinsMolecular Sequence DataMutagenesisRecombinant ProteinsRepressor ProteinsRestriction MappingSalmonella typhimuriumSequence Homology, Amino AcidTranscription FactorsTranscription, GeneticVirulenceConceptsCultured epithelial cellsSalmonella typhimurium genesNon-polar mutationT7 RNA polymerase-based expression systemEpithelial cellsAraC familyTranscription regulatorsInv locusTyphimurium genesNucleotide sequenceSalmonella entryInvGExpression systemInvFGenesProteinSequenceMutationsS. typhimuriumFamilyTranslocasesSimilar sizeCellsMembersHomologuesThe molecular genetic bases of Salmonella entry into mammalian cells
Galán J, Ginocchio C. The molecular genetic bases of Salmonella entry into mammalian cells. Biochemical Society Transactions 1994, 22: 301-306. PMID: 7958312, DOI: 10.1042/bst0220301.Peer-Reviewed Original ResearchContact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium
Ginocchio C, Olmsted S, Wells C, Galán J. Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium. Cell 1994, 76: 717-724. PMID: 8124710, DOI: 10.1016/0092-8674(94)90510-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineDogsEndocytosisEpitheliumGenes, BacterialIn Vitro TechniquesMicroscopy, Electron, ScanningSalmonella typhimuriumConceptsCultured epithelial cellsEpithelial cellsFormation of appendagesCell-signaling eventsDe novo protein synthesisNovo protein synthesisMembrane rufflesSurface organellesBacterial internalizationBacteria Salmonella typhimuriumSalmonella typhimuriumInternalization eventsSurface appendagesHost cellsNonphagocytic cellsS. typhimuriumProtein synthesisBacterial uptakeSuch appendagesSalmonella mutantsInternalization processIntimate interactionCellsBacteriaTyphimurium
1993
Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells
Altmeyer R, McNern J, Bossio JC, Rosenshine I, Finlay B, Galán J. Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells. Molecular Microbiology 1993, 7: 89-98. PMID: 8382333, DOI: 10.1111/j.1365-2958.1993.tb01100.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBacterial AdhesionBacterial ProteinsBase SequenceCells, CulturedDNA Transposable ElementsEpithelial CellsEpitheliumGenes, BacterialMolecular Sequence DataMutagenesisPhenotypeRecombinant Fusion ProteinsSalmonellaSalmonella typhimuriumSequence Homology, Nucleic AcidVirulenceConceptsCultured epithelial cellsInvH geneT7 RNA polymerase expression systemBacterial membrane fractionsEpithelial cellsSignal sequenceExtensive homologyColony hybridization analysisNucleotide sequenceGene productsHomologous sequencesExpression systemException of strainsMolecular characterizationGenesHybridization analysisMembrane fractionCultured cellsEscherichia coliPolypeptideInsertion sequence IS3E. coliOrganismsInvHSequence
1992
Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium
Galán J, Pace J, Hayman M. Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium. Nature 1992, 357: 588-589. PMID: 1608468, DOI: 10.1038/357588a0.Peer-Reviewed Original ResearchConceptsHenle-407 cellsEpidermal growth factor receptorMammalian cellsGrowth factor receptorTyrosine phosphorylationCultured mammalian cellsProtein tyrosine phosphorylationFactor receptorInternalization of SalmonellaCell surface receptorsCultured epithelial cellsAddition of EGFSuch phosphorylationSignal transductionSalmonella genesSalmonella typhimuriumEGF receptorS. typhimuriumIsogenic strainsEscherichia coliPhosphorylationEpithelial cellsGenesInternalizationInvasion
1990
Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains
Galán J, Nakayama K, Curtiss R. Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains. Gene 1990, 94: 29-35. PMID: 2227450, DOI: 10.1016/0378-1119(90)90464-3.Peer-Reviewed Original Research