2001
Tumor Necrosis Factor Alpha Increases Human Cerebral Endothelial Cell Gb3 and Sensitivity to Shiga Toxin
Eisenhauer P, Chaturvedi P, Fine R, Ritchie A, Pober J, Cleary T, Newburg D. Tumor Necrosis Factor Alpha Increases Human Cerebral Endothelial Cell Gb3 and Sensitivity to Shiga Toxin. Infection And Immunity 2001, 69: 1889-1894. PMID: 11179369, PMCID: PMC98098, DOI: 10.1128/iai.69.3.1889-1894.2001.Peer-Reviewed Original ResearchConceptsHemolytic uremic syndromeShiga toxinMicrovascular brain endothelial cellsCentral nervous system pathologyTumor necrosis factor alphaEndothelial cellsShiga toxin toxicityNervous system pathologyBrain endothelial cellsNecrosis factor alphaTNF-alpha treatmentUmbilical vein endothelial cellsCNS involvementVein endothelial cellsUremic syndromeIntestinal infectionsShiga Toxin BindingFactor alphaEnterohemorrhagic Escherichia coliSystem pathologyFunctional receptorsNormal fatty acidsToxin bindingFatty acidsToxin
2000
Human TNF Can Induce Nonspecific Inflammatory and Human Immune-Mediated Microvascular Injury of Pig Skin Xenografts in Immunodeficient Mouse Hosts
Kirkiles-Smith N, Tereb D, Kim R, McNiff J, Schechner J, Lorber M, Pober J, Tellides G. Human TNF Can Induce Nonspecific Inflammatory and Human Immune-Mediated Microvascular Injury of Pig Skin Xenografts in Immunodeficient Mouse Hosts. The Journal Of Immunology 2000, 164: 6601-6609. PMID: 10843720, DOI: 10.4049/jimmunol.164.12.6601.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAdultAnimalsCell Adhesion MoleculesDose-Response Relationship, ImmunologicDrug SynergismEndothelium, VascularGraft RejectionHistocompatibility AntigensHumansInterferon-gammaMiceMice, Inbred C57BLMice, SCIDMicrocirculationSevere Combined ImmunodeficiencySkin TransplantationSwineT-LymphocytesTransplantation, HeterologousTumor Necrosis Factor-alphaUp-RegulationConceptsHuman T-cell infiltrationPig skin xenograftsT cell infiltrationSkin xenograftsCell infiltrationHuman TNFIFN-gammaE-selectinT cell-mediated rejectionEndothelial cellsAdhesion moleculesCytokine-mediated injuryNonspecific inflammatory damagePig skin graftsCell-mediated rejectionAppropriate therapeutic targetsImmunodeficient mouse modelPorcine E-selectinEndothelial adhesion moleculesPorcine IFN-gammaClass II moleculesHuman skin allograftsMHC class IPorcine endothelial cellsImmunodeficient mouse hosts
1994
HLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons.
Johnson DR, Pober JS. HLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons. Molecular And Cellular Biology 1994, 14: 1322-1332. PMID: 8289810, PMCID: PMC358487, DOI: 10.1128/mcb.14.2.1322.Peer-Reviewed Original ResearchBase SequenceBinding SitesCloning, MolecularConsensus SequenceDNA PrimersDNA-Binding ProteinsDrug SynergismGene ExpressionGenes, MHC Class IHeLa CellsHLA-B7 AntigenHumansInterferon-betaInterferon-gammaLymphotoxin-alphaMolecular Sequence DataNF-kappa BNuclear ProteinsPolymerase Chain ReactionPromoter Regions, GeneticRecombinant ProteinsSequence DeletionSequence Homology, Nucleic AcidTransfection
1993
Endothelial activation by hydrogen peroxide. Selective increases of intercellular adhesion molecule-1 and major histocompatibility complex class I.
Bradley JR, Johnson DR, Pober JS. Endothelial activation by hydrogen peroxide. Selective increases of intercellular adhesion molecule-1 and major histocompatibility complex class I. American Journal Of Pathology 1993, 142: 1598-609. PMID: 8098585, PMCID: PMC1886909.Peer-Reviewed Original ResearchConceptsIntercellular adhesion molecule-1Tumor necrosis factorAdhesion molecule-1Major histocompatibility complex class I.Major histocompatibility complex class IHistocompatibility complex class IMolecule-1Complex class IInterleukin-1Intercellular adhesion molecule-1 surface expressionMumol/L hydrogen peroxideVascular cell adhesion molecule-1Transcription factor nuclear factor kappa BEndothelial leukocyte adhesion molecule-1Class ICell adhesion molecule-1Leukocyte-derived cytokinesNuclear factor kappa BVascular endothelial cell functionLeukocyte adhesion molecule-1Surface expressionClass I.Factor kappa BEC surface receptorsEndothelial cell function
1990
IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1.
Doukas J, Pober JS. IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1. The Journal Of Immunology 1990, 145: 1727-33. PMID: 1697308, DOI: 10.4049/jimmunol.145.6.1727.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, NorthernCell Adhesion MoleculesDrug SynergismEndothelium, VascularE-SelectinFluorescent Antibody TechniqueGene ExpressionHistocompatibility Antigens Class IHumansIn Vitro TechniquesIntercellular Adhesion Molecule-1Interferon-gammaInterleukin-1Recombinant ProteinsRNA, MessengerTumor Necrosis Factor-alphaConceptsEndothelial leukocyte adhesion molecule-1ELAM-1 expressionIL-1 betaIFN-gammaIL-1Endothelial cellsEC activationIL-1 beta responseIntercellular adhesion molecule-1 expressionAdhesion molecule-1 expressionMHC Ag expressionLeukocyte adhesion molecule-1Treatment of ECICAM-1 expressionMolecule-1 expressionClass I MHC moleculesTumor necrosis factorAdhesion molecule-1ICAM-1 mRNAClass I MHCI MHC moleculesIFN-gamma effectELAM-1 mRNACultured endothelial cellsIncrease TNFTumor necrosis factor and immune interferon synergistically increase transcription of HLA class I heavy- and light-chain genes in vascular endothelium.
Johnson DR, Pober JS. Tumor necrosis factor and immune interferon synergistically increase transcription of HLA class I heavy- and light-chain genes in vascular endothelium. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 5183-5187. PMID: 2164225, PMCID: PMC54286, DOI: 10.1073/pnas.87.13.5183.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell NucleusCells, CulturedDrug SynergismEndothelium, VascularFlow CytometryGenes, MHC Class IHistocompatibility Antigens Class IHumansInterferon Type IInterferon-gammaMacromolecular SubstancesMolecular Sequence DataReceptors, Cell SurfaceReceptors, Tumor Necrosis FactorRecombinant ProteinsRNA, MessengerSequence Homology, Nucleic AcidTranscription, GeneticTumor Necrosis Factor-alphaConceptsNecrosis factorHuman endothelial cellsEndothelial cellsClass IInterferon gammaImmune interferonMRNA levelsMajor histocompatibility complex moleculesTumor necrosis factorHLA class IClass I major histocompatibility complex moleculesCultured human endothelial cellsHistocompatibility complex moleculesUntreated endothelial cellsSynergistic increase
1989
IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis.
Zavoico GB, Ewenstein BM, Schafer AI, Pober JS. IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis. The Journal Of Immunology 1989, 142: 3993-9. PMID: 2497185, DOI: 10.4049/jimmunol.142.11.3993.Peer-Reviewed Original ResearchMeSH KeywordsArachidonic AcidArachidonic AcidsCells, CulturedChromatography, Thin LayerCycloheximideDrug SynergismEndothelium, VascularEpoprostenolHistamineHumansInterleukin-1Platelet Activating FactorProstaglandin Endoperoxides, SyntheticProstaglandin H2Prostaglandins HThrombinVon Willebrand Factor
1988
Three distinct classes of regulatory cytokines control endothelial cell MHC antigen expression. Interactions with immune gamma interferon differentiate the effects of tumor necrosis factor and lymphotoxin from those of leukocyte alpha and fibroblast beta interferons.
Lapierre LA, Fiers W, Pober JS. Three distinct classes of regulatory cytokines control endothelial cell MHC antigen expression. Interactions with immune gamma interferon differentiate the effects of tumor necrosis factor and lymphotoxin from those of leukocyte alpha and fibroblast beta interferons. Journal Of Experimental Medicine 1988, 167: 794-804. PMID: 2450953, PMCID: PMC2188900, DOI: 10.1084/jem.167.3.794.Peer-Reviewed Original ResearchConceptsIFN-alphaIL-6Endothelial cellsClass IAction of lymphotoxinMHC antigen expressionIFN-alpha/betaClass II antigensClass I expressionClass II inductionTumor necrosis factorCultured human endothelial cellsClass I MHCRecombinant preparationsHuman endothelial cellsCytokine administrationMHC antigensII antigensPatient populationRegulatory cytokinesNecrosis factorAntigen expressionGamma interferonIFN-gammaI MHC