2011
Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells
Lebastchi AH, Khan SF, Qin L, Li W, Zhou J, Hibino N, Yi T, Rao DA, Pober JS, Tellides G. Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells. American Journal Of Transplantation 2011, 11: 2332-2341. PMID: 21812925, PMCID: PMC3203343, DOI: 10.1111/j.1600-6143.2011.03676.x.Peer-Reviewed Original ResearchConceptsEffector T cellsMemory T cellsNaïve T cellsT cellsIL-17Vascular cellsEffector memory T cellsAlloreactive memory T cellsGrowth factor-beta expressionMedial smooth muscle cellsHuman effector memory T cellsHuman coronary artery graftsIL-17 productionCoronary artery graftsIFN-γ productionAllogeneic endothelial cellsInterferon-gamma productionDevelopment of arteriosclerosisExpression of TGFRole of TGFSmooth muscle cellsImmunodeficient mouse hostsHuman T cellsIntimal compartmentMedial infiltration
2000
Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype
Dengler T, Pober J. Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype. The Journal Of Immunology 2000, 164: 5146-5155. PMID: 10799873, DOI: 10.4049/jimmunol.164.10.5146.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCoculture TechniquesCyclosporineCytotoxicity Tests, ImmunologicEndothelium, VascularHistocompatibility Antigens Class IHumansImmunologic MemoryImmunophenotypingInterleukin-12InterphaseLeukocyte Common AntigensLymphocyte ActivationT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicConceptsB lymphoblastoid cellsT cellsNaive CD8Endothelial cellsImmunoregulatory cell typesIntracellular perforin contentAlloreactive T cellsAnti-CD28 mAbHuman vascular endothelial cellsHigh surface expressionVascular endothelial cellsExpansion of memoryConventional CTLGraft parenchymaGraft rejectionMemory CD8CTL generationPerforin contentCTL expansionVascular injuryHuman CD8CD40 ligandAlloreactive CTLAnatomic compartmentsICAM-1Large-Scale Culture and Selective Maturation of Human Langerhans Cells from Granulocyte Colony-Stimulating Factor-Mobilized CD34+ Progenitors
Gatti E, Velleca M, Biedermann B, Ma W, Unternaehrer J, Ebersold M, Medzhitov R, Pober J, Mellman I. Large-Scale Culture and Selective Maturation of Human Langerhans Cells from Granulocyte Colony-Stimulating Factor-Mobilized CD34+ Progenitors. The Journal Of Immunology 2000, 164: 3600-3607. PMID: 10725716, DOI: 10.4049/jimmunol.164.7.3600.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, CD1Antigens, CD34Antigens, Differentiation, T-LymphocyteAntigens, NeoplasmCD40 LigandCell CountCell Culture TechniquesCell DifferentiationDendritic CellsGranulocyte Colony-Stimulating FactorHematopoietic Stem Cell TransplantationHumansImmunophenotypingLangerhans CellsLeukapheresisLigandsLipopolysaccharidesMembrane GlycoproteinsStem CellsTumor Necrosis Factor-alphaConceptsDendritic cellsLangerhans cellsT cell stimulationG-CSF-mobilized patientsManipulation of DCsImmature dendritic cellsIL-12 productionPrimary immune responseHuman Langerhans cellsCell stimulationDifferent proinflammatory stimuliToll family receptorsDC preparationsMaturation stimuliTNF-alphaCD40 ligandMHC productsImmune responseBirbeck granulesProinflammatory stimuliCytokine removalFamily receptorsAg processingDC typesHomogenous population
1999
Human vascular endothelial cells favor clonal expansion of unusual alloreactive CTL.
Biedermann B, Pober J. Human vascular endothelial cells favor clonal expansion of unusual alloreactive CTL. The Journal Of Immunology 1999, 162: 7022-30. PMID: 10358144, DOI: 10.4049/jimmunol.162.12.7022.Peer-Reviewed Original ResearchConceptsTarget cell contactB lymphoblastoid cellsLittle IFN-gammaIFN-gammaEndothelial cellsCTL clonesClass I MHC-restricted CTLAbsence of ECExpression of CD40LAllogeneic endothelial cellsIFN-gamma secretionIL-4 secretionCell contactHuman vascular endothelial cellsVascular endothelial cellsCTL subsetsCD40L expressionCTL linesCD40 ligandT cellsAlloreactive CTLArterial intimaClonal expansionCTLCultured HUVECs
1998
Human endothelial cells induce and regulate cytolytic T cell differentiation.
Biedermann B, Pober J. Human endothelial cells induce and regulate cytolytic T cell differentiation. The Journal Of Immunology 1998, 161: 4679-87. PMID: 9794397, DOI: 10.4049/jimmunol.161.9.4679.Peer-Reviewed Original ResearchConceptsB lymphoblastoid cellsEndothelial cellsHuman endothelial cellsSame donorT cellsProliferation of CD8IFN-gamma secretionCultured human endothelial cellsT cell differentiationT cell growthAllogeneic CD8CTL responsesPerforin expressionCTL differentiationPresence of ECT lymphocytesAllospecific CTLCD8CTLEfficient stimulatorsLymphoblastoid cellsCell numberReproducible frequencyCell growthInput cell numberHuman endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells.
Ma W, Pober J. Human endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells. The Journal Of Immunology 1998, 161: 2158-67. PMID: 9725207, DOI: 10.4049/jimmunol.161.5.2158.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAdultCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell DifferentiationCells, CulturedCytokinesEndothelium, VascularHumansImmunologic MemoryInterferon-gammaInterleukin-12Interleukin-2Interleukin-4InterphaseLeukocytes, MononuclearLymphocyte ActivationTh1 CellsT-Lymphocyte SubsetsUmbilical VeinsConceptsPeripheral blood adherent mononuclear cellsExogenous IL-12Naive T cellsT cellsIL-12Endothelial cellsMemory CD4Human endothelial cellsCytokine productionNaive CD4B7 moleculesIL-2IFN-gammaBone marrow-derived APCsBlood adherent mononuclear cellsB7 molecule expressionIL-12 supplementationIntracellular cytokine stainingTh1-like cellsIL-4 productionIFN-gamma productionCytokine stainingMononuclear cellsIL-4Molecule expressionEndothelial cells in physiology and in the pathophysiology of vascular disorders.
Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, Pober JS, Wick TM, Konkle BA, Schwartz BS, Barnathan ES, McCrae KR, Hug BA, Schmidt AM, Stern DM. Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 1998, 91: 3527-61. PMID: 9572988.Peer-Reviewed Original ResearchAnimalsArteriosclerosisBlood CellsBlood CoagulationCell AdhesionCell Adhesion MoleculesCell DifferentiationCells, CulturedEndothelial Growth FactorsEndothelinsEndothelium, VascularEpoprostenolExtracellular MatrixFemaleFibrinolysisFibroblast Growth Factor 2HemodynamicsHumansInflammationLymphokinesMaleMiceNeovascularization, PhysiologicNitric OxidePlatelet Activating FactorPre-EclampsiaPregnancyThrombosisUmbilical VeinsVascular DiseasesVascular Endothelial Growth Factor AVascular Endothelial Growth Factors
1988
Immortalization of human endothelial cells by murine sarcoma viruses, without morphologic transformation
Faller D, Kourembanas S, Ginsberg D, Hannan R, Collins T, Ewenstein B, Pober J, Tantravahi R. Immortalization of human endothelial cells by murine sarcoma viruses, without morphologic transformation. Journal Of Cellular Physiology 1988, 134: 47-56. PMID: 2826502, DOI: 10.1002/jcp.1041340106.Peer-Reviewed Original Research