2014
Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2
Wang C, Qin L, Manes TD, Kirkiles-Smith NC, Tellides G, Pober JS. Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2. Journal Of Experimental Medicine 2014, 211: 395-404. PMID: 24516119, PMCID: PMC3949571, DOI: 10.1084/jem.20131125.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceBlotting, WesternCell AdhesionChromatin ImmunoprecipitationDNA PrimersEndothelial CellsFlow CytometryHumansImmunoblottingMechanistic Target of Rapamycin Complex 2Microscopy, FluorescenceMultiprotein ComplexesOncogene Protein v-aktReal-Time Polymerase Chain ReactionSirolimusT-LymphocytesTOR Serine-Threonine KinasesTumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1VCAM-1 expressionEndothelial cellsActivation of ERK1/2Cell adhesion molecule-1TNF inductionInfiltration of leukocytesAdhesion molecule-1Inhibition of TNFPotential therapeutic targetAbility of rapamycinAbility of TNFTranscription factor IRF-1Hyperactivation of ERK1/2Inhibition of ERK1/2Venular flowT cellsEndothelial expressionInflamed tissuesVascular endotheliumMolecule-1Therapeutic targetRapamycin pretreatmentRenal glomeruliTNF
2013
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment
Lauridsen HM, Pober JS, Gonzalez AL. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment. The FASEB Journal 2013, 28: 1166-1180. PMID: 24297702, PMCID: PMC3929680, DOI: 10.1096/fj.13-240986.Peer-Reviewed Original ResearchConceptsAdhesion molecule-1Cell adhesion molecule-1Molecule-1Endothelial cellsPostcapillary venulesBasement membraneVascular cell adhesion molecule-1Intercellular adhesion molecule-1Tumor necrosis factor αMicrovascular leukocyte recruitmentNecrosis factor αLate antigen-4Platelet endothelial cell adhesion molecule-1TNF-α activationInflammatory cascadeAntigen-4Neutrophil extravasationInterleukin-8Leukocyte recruitmentNeutrophil adhesionFactor αTNFPericytesVenular shear stressAnti-CD99
1998
Human endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells.
Ma W, Pober J. Human endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells. The Journal Of Immunology 1998, 161: 2158-67. PMID: 9725207, DOI: 10.4049/jimmunol.161.5.2158.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAdultCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell DifferentiationCells, CulturedCytokinesEndothelium, VascularHumansImmunologic MemoryInterferon-gammaInterleukin-12Interleukin-2Interleukin-4InterphaseLeukocytes, MononuclearLymphocyte ActivationTh1 CellsT-Lymphocyte SubsetsUmbilical VeinsConceptsPeripheral blood adherent mononuclear cellsExogenous IL-12Naive T cellsT cellsIL-12Endothelial cellsMemory CD4Human endothelial cellsCytokine productionNaive CD4B7 moleculesIL-2IFN-gammaBone marrow-derived APCsBlood adherent mononuclear cellsB7 molecule expressionIL-12 supplementationIntracellular cytokine stainingTh1-like cellsIL-4 productionIFN-gamma productionCytokine stainingMononuclear cellsIL-4Molecule expressionEndothelial cells in physiology and in the pathophysiology of vascular disorders.
Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, Pober JS, Wick TM, Konkle BA, Schwartz BS, Barnathan ES, McCrae KR, Hug BA, Schmidt AM, Stern DM. Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 1998, 91: 3527-61. PMID: 9572988.Peer-Reviewed Original ResearchAnimalsArteriosclerosisBlood CellsBlood CoagulationCell AdhesionCell Adhesion MoleculesCell DifferentiationCells, CulturedEndothelial Growth FactorsEndothelinsEndothelium, VascularEpoprostenolExtracellular MatrixFemaleFibrinolysisFibroblast Growth Factor 2HemodynamicsHumansInflammationLymphokinesMaleMiceNeovascularization, PhysiologicNitric OxidePlatelet Activating FactorPre-EclampsiaPregnancyThrombosisUmbilical VeinsVascular DiseasesVascular Endothelial Growth Factor AVascular Endothelial Growth Factors
1996
Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA‐3 signaling pathway
Karmann K, Hughes C, Fanslow W, Pober J. Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA‐3 signaling pathway. European Journal Of Immunology 1996, 26: 610-617. PMID: 8605928, DOI: 10.1002/eji.1830260316.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, Differentiation, T-LymphocyteB-LymphocytesCD2 AntigensCD40 LigandCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell CommunicationCells, CulturedEndothelium, VascularHumansInterleukin-2LigandsLymphocyte ActivationMembrane GlycoproteinsPhytohemagglutininsSignal TransductionUp-RegulationConceptsCD40 ligand expressionExogenous IL-2T cellsIL-2Ligand expressionCD2/LFACD40 ligandHuman peripheral blood CD4Peripheral blood adherent cellsHelper T cell subsetsPeripheral blood T cellsPeripheral blood CD4T cell subsetsCo-stimulatory moleculesBlood T cellsHelper T cellsIL-2 receptorCo-stimulatory signalsIL-2 secretionInterleukin-2 secretionBlood CD4Endothelial effectsCell subsetsHuman CD4B cells
1994
Endothelial cell-induced resistance to cyclosporin A in human peripheral blood T cells requires contact-dependent interactions involving CD2 but not CD28.
Karmann K, Pober JS, Hughes CC. Endothelial cell-induced resistance to cyclosporin A in human peripheral blood T cells requires contact-dependent interactions involving CD2 but not CD28. The Journal Of Immunology 1994, 153: 3929-37. PMID: 7523510, DOI: 10.4049/jimmunol.153.9.3929.Peer-Reviewed Original ResearchConceptsIL-2 secretionT cellsEndothelial cellsPeripheral blood T cellsHuman peripheral blood T cellsGraft endothelial cellsHost T cellsBlood T cellsT cell CD28Cultured human endothelial cellsEndothelial cell ligandsIL-2 synthesisInitiation of cocultureContact-dependent interactionsEffect of ECHuman endothelial cellsT cell CD2Presence of ECInhibitory doseLFA-3Cell ligandsCyclosporin ACD28Cell contactSecretionProlonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells
Birch K, Ewenstein B, Golan D, Pober J. Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells. Journal Of Cellular Physiology 1994, 160: 545-554. PMID: 7521337, DOI: 10.1002/jcp.1041600318.Peer-Reviewed Original ResearchConceptsEndothelial cellsAgonist concentrationsSingle human umbilical vein endothelial cellCytoplasmic free calcium ionsNumber of cellsPeak elevationHigh agonist concentrationsCytoplasmic free calciumHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVWF secretionIndividual endothelial cellsAmount of secretionVein endothelial cellsHuman endothelial cellsAgonist treatmentVon Willebrand factor secretionAgonist doseExtracellular Ca2Maximal secretionP-selectinThrombin receptorHigh dosesIntracellular storesFactor secretionInducibility and Expression of Microvascular Endothelial Adhesion Molecules in Lesional, Perilesional, and Uninvolved Skin of Psoriatic Patients
Petzelbauer P, Pober J, Keh A, Braverman I. Inducibility and Expression of Microvascular Endothelial Adhesion Molecules in Lesional, Perilesional, and Uninvolved Skin of Psoriatic Patients. Journal Of Investigative Dermatology 1994, 103: 300-305. PMID: 7521374, DOI: 10.1111/1523-1747.ep12394720.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Endothelial leukocyte adhesion molecule-1Lesional skinAdhesion molecule-1Psoriatic patientsEndothelial cellsUninvolved skinPerilesional skinMolecule-1Dermal microvasculatureCapillary loopsCell adhesion molecule-1Perivascular inflammatory infiltratesLeukocyte adhesion molecule-1VCAM-1 expressionTumor necrosis factorEndothelial adhesion moleculesMicrovascular endothelial cellsEndothelial cell expressionVenular endothelial cellsInflammatory infiltrateEpidermal involvementPsoriatic plaquesSuperficial plexusNecrosis factor
1993
Elevated cyclic AMP inhibits endothelial cell synthesis and expression of TNF-induced endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1.
Pober JS, Slowik MR, De Luca LG, Ritchie AJ. Elevated cyclic AMP inhibits endothelial cell synthesis and expression of TNF-induced endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1. The Journal Of Immunology 1993, 150: 5114-23. PMID: 7684420, DOI: 10.4049/jimmunol.150.11.5114.Peer-Reviewed Original ResearchConceptsEndothelial cellsAction of cAMPCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsSignal transducerUmbilical vein endothelial cellsRole of cAMPCell adhesion molecule-1Adhesion molecule-1Vein endothelial cellsCyclic monophosphatePhysiological regulatorElevated cyclic AMPVascular cell adhesion molecule-1Adhesion molecule expressionIntracellular mediatorsIsobutyl methylxanthineMolecule-1TNF inductionVCAM-1 mRNA
1991
Expression of endothelial leukocyte adhesion molecule-1 in septic but not traumatic/hypovolemic shock in the baboon.
Redl H, Dinges HP, Buurman WA, van der Linden CJ, Pober JS, Cotran RS, Schlag G. Expression of endothelial leukocyte adhesion molecule-1 in septic but not traumatic/hypovolemic shock in the baboon. American Journal Of Pathology 1991, 139: 461-6. PMID: 1714243, PMCID: PMC1886066.Peer-Reviewed Original ResearchConceptsEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Hypovolemic shockSeptic shockAdhesion molecule-1Endothelial leukocyte adhesion molecule-1 expressionMolecule-1Adhesion molecule-1 expressionGranulocyte elastase levelsMolecule-1 expressionELAM-1 expressionTumor necrosis factorDe novo expressionEndothelial activationElastase levelsLive Escherichia coliNeutrophil activationNecrosis factorImmunohistochemical techniquesELAM-1ELAM expressionStrong stainingNovo expressionSmall veinsHigh levelsExpression of endothelial-leukocyte adhesion molecule-1 in elicited late phase allergic reactions.
Leung DY, Pober JS, Cotran RS. Expression of endothelial-leukocyte adhesion molecule-1 in elicited late phase allergic reactions. Journal Of Clinical Investigation 1991, 87: 1805-1809. PMID: 1708785, PMCID: PMC295299, DOI: 10.1172/jci115201.Peer-Reviewed Original ResearchConceptsLate-phase reactionInflammatory cellsAllergic reactionsEC activationLate-phase allergic reactionsEndothelial leukocyte adhesion molecule-1Adhesion moleculesIntradermal allergen injectionSequential skin biopsiesInflammatory cell infiltrateNeutrophil adhesion moleculesEndothelial leukocyte adhesion moleculeAllergic skin reactionsELAM-1 expressionOrgan cultureAdhesion molecule-1Skin organ cultureAtopic patientsCell infiltrateEndothelial activationAllergen injectionRespiratory allergySkin reactionsTNF-alphaIntradermal injectionWhat can be learned from the expression of endothelial adhesion molecules in tissues?
Pober J, Cotran RS. What can be learned from the expression of endothelial adhesion molecules in tissues? Laboratory Investigation 1991, 64: 301-5. PMID: 2002650.Peer-Reviewed Original Research
1990
THE ROLE OF ENDOTHELIAL CELLS IN INFLAMMATION
POBER J, COTRAN R. THE ROLE OF ENDOTHELIAL CELLS IN INFLAMMATION. Transplantation 1990, 50: 537-544. PMID: 2219269, DOI: 10.1097/00007890-199010000-00001.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsCytokine-endothelial interactions in inflammation, immunity, and vascular injury.
Cotran RS, Pober JS. Cytokine-endothelial interactions in inflammation, immunity, and vascular injury. Journal Of The American Society Of Nephrology 1990, 1: 225-35. PMID: 2104268, DOI: 10.1681/asn.v13225.Peer-Reviewed Original ResearchConceptsVascular injuryTumor necrosis factorEffects of cytokinesEndothelial adhesion moleculesLeukocyte-endothelial adhesionCytokine-induced activationSuch pathological processesSeptic shockNecrosis factorImmune responseInterleukin-1Shwartzman reactionAdhesion moleculesPathological processesCytokinesInjuryStructural alterationsBiological characteristicsPossible contributionVasculitisInflammationEndotheliumHypersensitivityImmunityImportant roleAdhesion of Human B Cells to Germinal Centers in Vitro Involves VLA-4 and INCAM-110
Freedman A, Munro J, Rice G, Bevilacqua M, Morimoto C, McIntyre B, Rhynhart K, Pober J, Nadler L. Adhesion of Human B Cells to Germinal Centers in Vitro Involves VLA-4 and INCAM-110. Science 1990, 249: 1030-1033. PMID: 1697696, DOI: 10.1126/science.1697696.Peer-Reviewed Original Research
1989
ENDOTHELIAL CELL ACTIVATION AND HIGH INTERLEUKIN-1 SECRETION IN THE PATHOGENESIS OF ACUTE KAWASAKI DISEASE
Leung D, Kurt-Jones E, Newburger J, Cotran R, Burns J, Pober J. ENDOTHELIAL CELL ACTIVATION AND HIGH INTERLEUKIN-1 SECRETION IN THE PATHOGENESIS OF ACUTE KAWASAKI DISEASE. The Lancet 1989, 334: 1298-1302. PMID: 2480498, DOI: 10.1016/s0140-6736(89)91910-7.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, SurfaceCell AdhesionCell Adhesion MoleculesChild, PreschoolEndothelium, VascularE-SelectinFemaleGamma-GlobulinsHLA-DQ AntigensHumansImmunization, PassiveIntercellular Adhesion Molecule-1Interleukin-1MaleMembrane GlycoproteinsMucocutaneous Lymph Node SyndromeReceptors, ImmunologicSkinConceptsEndothelial cell activationCoronary artery abnormalitiesCell activationInterleukin-1Gammaglobulin treatmentArtery abnormalitiesKawasaki diseaseAcute Kawasaki disease patientsPeripheral blood mononuclear cellsEndothelial cellsCytotoxic antibody activityIntravenous gammaglobulin treatmentKawasaki disease patientsAcute Kawasaki diseaseEndothelial cell antigensBlood mononuclear cellsInterleukin-1 secretionTumor necrosis factorIL-1 secretionEndothelial cell expressionSkin biopsy samplesMeans of immunoperoxidaseMucocutaneous symptomsPersistent feverClinical symptoms
1987
Identification of an inducible endothelial-leukocyte adhesion molecule.
Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA. Identification of an inducible endothelial-leukocyte adhesion molecule. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 9238-9242. PMID: 2827173, PMCID: PMC299728, DOI: 10.1073/pnas.84.24.9238.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell surface proteinsEndothelial cell surface proteinsUnstimulated human endothelial cellsCell surface structuresHL-60 cellsSame polypeptideHL-60 adhesionCultured human endothelial cellsAdhesion assaysAdhesive interactionsH18/7Adhesion moleculesCytokine-stimulated human endothelial cellsDependent fashionCell surface antigensEndothelial cellsEndothelial leukocyte adhesion moleculeEndothelial leukocyte adhesion molecule-1AdhesionCellsAdhesion molecule-1Monoclonal antibodiesSites of inflammationLesser extentEffects of Tumour Necrosis Factor and Related Cytokines on Vascular Endothelial Cells
Pober J. Effects of Tumour Necrosis Factor and Related Cytokines on Vascular Endothelial Cells. Novartis Foundation Symposia 1987, 131: 170-191. PMID: 3330009, DOI: 10.1002/9780470513521.ch12.Peer-Reviewed Original ResearchConceptsIL-1 betaHuman endothelial cellsIL-1 alphaRelated cytokinesVascular endothelial cellsEndothelial cellsNecrosis factorEndothelial leucocyte adhesion moleculeIntercellular adhesion molecule-1Tumor necrosis factorInterleukin-1 betaAdhesion molecule-1Endothelial cell activationInterleukin-1 alphaCultured human endothelial cellsPhorbol esterEffects of TNFRecombinant human TNFCytoplasmic calcium concentrationIL-1 speciesMechanism of actionHEC adhesivenessInflammatory leucocytesCell surface expressionProtein kinase C pathway
1986
Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon.
Pober JS, Gimbrone MA, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. The Journal Of Immunology 1986, 137: 1893-6. PMID: 3091693, DOI: 10.4049/jimmunol.137.6.1893.Peer-Reviewed Original ResearchConceptsTumor necrosis factorIFN-gammaHuman umbilical vein endothelialNecrosis factorIL-1Interleukin-1HUVE cellsImmune interferonIntercellular adhesion molecule-1Endothelial cellsDifferent immune mediatorsAdhesion molecule-1Murine monoclonal antibodiesHuman endothelial cellsImmune mediatorsPattern of activationActivation antigensAntigenic modulationB antigensDifferent lymphokinesMolecule-1Monoclonal antibodiesProgressive increaseInterferonAntigen
1985
Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines.
Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines. Journal Of Clinical Investigation 1985, 76: 2003-2011. PMID: 3877078, PMCID: PMC424265, DOI: 10.1172/jci112200.Peer-Reviewed Original ResearchConceptsEndothelial-leukocyte adhesionHuman vascular endotheliumIL-1Leukocyte adhesionVascular endotheliumCultured human vascular endotheliumLeukocyte-vessel wall interactionsIL-1 pretreatmentEndothelial cell damageEndothelial monolayersSites of inflammationIL-1 treatmentHuman umbilical vein endothelial monolayersCertain vascular diseasesPromyelocytic cell line HL-60Polymorphonuclear leukocyte adhesionIL-1 inductionImmune mediatorsHuman endothelial cellsEndothelial adhesivityVascular diseaseBlood leukocytesInterleukin-1Leukocyte cell linesCell line U937