2021
Complement‐activated human endothelial cells stimulate increased polyfunctionality in alloreactive T cells
Xie CB, Zhou J, Mackay S, Pober JS. Complement‐activated human endothelial cells stimulate increased polyfunctionality in alloreactive T cells. American Journal Of Transplantation 2021, 21: 1902-1909. PMID: 33415805, PMCID: PMC8096653, DOI: 10.1111/ajt.16485.Peer-Reviewed Original ResearchMeSH KeywordsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesComplement System ProteinsCytokinesEndothelial CellsHumansLymphocyte ActivationT-Lymphocytes
2020
Complement activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cells
Xie CB, Jiang B, Qin L, Tellides G, Kirkiles-Smith NC, Jane-wit D, Pober JS. Complement activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cells. Journal Of Clinical Investigation 2020, 130: 3437-3452. PMID: 32191642, PMCID: PMC7324183, DOI: 10.1172/jci135060.Peer-Reviewed Original ResearchConceptsIL-15/IL-15Rα complexesIL-1βHuman endothelial cellsMembrane attack complexEndothelial cellsAcute rejectionT cellsT cell-mediated acute rejectionCell-mediated acute rejectionComplement-mediated pathologiesIL-15Rα expressionGraft endothelial cellsHuman coronary artery graftsEffector memory CD4T cell infiltrationCoronary artery graftsIL-1 receptorActive IL-1βCultured human endothelial cellsNLRP3 inflammasome assemblyNoncanonical NF-κBArtery graftAlloreactive CD8Complement membrane attack complexMemory CD4
2009
Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death
Choy JC, Pober JS. Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death. American Journal Of Transplantation 2009, 9: 2281-2291. PMID: 19663890, PMCID: PMC3505447, DOI: 10.1111/j.1600-6143.2009.02771.x.Peer-Reviewed Original ResearchConceptsHuman CD8 T cellsCD8 T cellsInducible NO synthaseT cellsActivated T cellsNitric oxideDeprivation-induced cell deathCell deathAllogeneic endothelial cellsT cell proliferationActivation-induced cell deathCytokine deprivationGeneration of NOT cell deathExogenous nitric oxideHuman T cellsNO augmentsAllogeneic responseS-nitrosylationNO synthaseProtective effectActivity of caspasesEndothelial cellsPharmacological inhibitorsDeath
2001
Human Vascular Endothelial Cells Stimulate a Lower Frequency of Alloreactive CD8+ Pre-CTL and Induce Less Clonal Expansion than Matching B Lymphoblastoid Cells: Development of a Novel Limiting Dilution Analysis Method Based on CFSE Labeling of Lymphocytes
Dengler T, Johnson D, Pober J. Human Vascular Endothelial Cells Stimulate a Lower Frequency of Alloreactive CD8+ Pre-CTL and Induce Less Clonal Expansion than Matching B Lymphoblastoid Cells: Development of a Novel Limiting Dilution Analysis Method Based on CFSE Labeling of Lymphocytes. The Journal Of Immunology 2001, 166: 3846-3854. PMID: 11238628, DOI: 10.4049/jimmunol.166.6.3846.Peer-Reviewed Original ResearchAntigens, CDB-Lymphocyte SubsetsCD8-Positive T-LymphocytesCell DivisionCell Line, TransformedCells, CulturedClone CellsCoculture TechniquesColony-Forming Units AssayCytotoxicity Tests, ImmunologicEndothelium, VascularFlow CytometryFluoresceinsFluorescent DyesHumansImmunologic MemoryInterphaseIsoantigensLymphocyte ActivationLymphocyte CountStem CellsSuccinimidesT-Lymphocyte SubsetsT-Lymphocytes, Regulatory
2000
Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype
Dengler T, Pober J. Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype. The Journal Of Immunology 2000, 164: 5146-5155. PMID: 10799873, DOI: 10.4049/jimmunol.164.10.5146.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCoculture TechniquesCyclosporineCytotoxicity Tests, ImmunologicEndothelium, VascularHistocompatibility Antigens Class IHumansImmunologic MemoryImmunophenotypingInterleukin-12InterphaseLeukocyte Common AntigensLymphocyte ActivationT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicConceptsB lymphoblastoid cellsT cellsNaive CD8Endothelial cellsImmunoregulatory cell typesIntracellular perforin contentAlloreactive T cellsAnti-CD28 mAbHuman vascular endothelial cellsHigh surface expressionVascular endothelial cellsExpansion of memoryConventional CTLGraft parenchymaGraft rejectionMemory CD8CTL generationPerforin contentCTL expansionVascular injuryHuman CD8CD40 ligandAlloreactive CTLAnatomic compartmentsICAM-1
1998
Dermal Microvascular Injury in the Human Peripheral Blood Lymphocyte Reconstituted-Severe Combined Immunodeficient (HuPBL-SCID) Mouse/Skin Allograft Model Is T Cell Mediated and Inhibited by a Combination of Cyclosporine and Rapamycin
Murray A, Schechner J, Epperson D, Sultan P, McNiff J, Hughes C, Lorber M, Askenase P, Pober J. Dermal Microvascular Injury in the Human Peripheral Blood Lymphocyte Reconstituted-Severe Combined Immunodeficient (HuPBL-SCID) Mouse/Skin Allograft Model Is T Cell Mediated and Inhibited by a Combination of Cyclosporine and Rapamycin. American Journal Of Pathology 1998, 153: 627-638. PMID: 9708821, PMCID: PMC1852982, DOI: 10.1016/s0002-9440(10)65604-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesCD8-Positive T-LymphocytesCyclosporineDrug Therapy, CombinationEndothelium, VascularEnzyme-Linked Immunosorbent AssayFlow CytometryGenes, MHC Class IIGraft RejectionHumansImmunosuppressive AgentsKeratinocytesMiceMice, SCIDMicrocirculationPolyenesSirolimusSkinSkin TransplantationT-LymphocytesTransplantation, HomologousVascular Cell Adhesion Molecule-1ConceptsPeripheral blood mononuclear cellsHuman peripheral blood mononuclear cellsSkin allograft modelMicrovascular injuryT cellsCell infiltrateAllograft modelWhole peripheral blood mononuclear cellsT cell-dependent mechanismT cell-mediated rejectionHuman natural killer cellsSCID/beige miceEndothelial cell sloughingT Cell-MediatedCell-mediated rejectionCombination of cyclosporineT-cell infiltratesCell-dependent mechanismMononuclear cell infiltrateNatural killer cellsMononuclear cell infiltrationBlood mononuclear cellsSkin graft modelHuman immune responseImmunoglobulin G antibodies
1997
Vascular cells have limited capacities to activate and differentiate T cells: Implications for transplant vascular sclerosis
Pober J, Ma W, Biedermann B, Libby P. Vascular cells have limited capacities to activate and differentiate T cells: Implications for transplant vascular sclerosis. Transplant Immunology 1997, 5: 251-254. PMID: 9504143, DOI: 10.1016/s0966-3274(97)80004-4.Peer-Reviewed Original Research
1994
Antigen-presenting function of human endothelial cells. Direct activation of resting CD8 T cells.
Epperson DE, Pober JS. Antigen-presenting function of human endothelial cells. Direct activation of resting CD8 T cells. The Journal Of Immunology 1994, 153: 5402-12. PMID: 7989746, DOI: 10.4049/jimmunol.153.12.5402.Peer-Reviewed Original ResearchConceptsCD8 T cellsMemory CD8 T cellsT cell proliferative responsesAllogeneic endothelial cellsCell proliferative responsesT cellsAdhesion molecule-1Endothelial cellsCD8/IL-2Proliferative responseMolecule-1CD4 T cell proliferative responsesLate activation Ag-4Human CD8 T cellsVascular cell adhesion molecule-1Class II MHC expressionIntercellular adhesion molecule-1Cell adhesion molecule-1CD4 T cellsIFN-gamma secretionOrgan graft rejectionIL-2R alpha subunitIntercellular adhesion molecule-2CD8 cellsPorcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28
Murray A, Khodedoust M, Pober J, Bothwell A. Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28. Immunity 1994, 1: 57-63. PMID: 7889399, DOI: 10.1016/1074-7613(94)90009-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationAntigens, HeterophileCD2 AntigensCD28 AntigensCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansIn Vitro TechniquesLigandsLymphocyte ActivationSpecies SpecificitySwineT-LymphocytesConceptsPorcine aortic endothelial cellsHuman umbilical vein endothelial cellsHuman T cellsCTLA-4-IgT cellsAortic endothelial cellsEndothelial cellsCultured porcine aortic endothelial cellsMHC class II moleculesClass II moleculesMHC class IUmbilical vein endothelial cellsVein endothelial cellsAllogeneic responseMHC antigensHuman CD8CD28 pathwayCD8 moleculesHuman CD4Species-specific monoclonal antibodiesCD2 pathwayMonoclonal antibodiesClass IDirect presentationCD4