1994
Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells
Birch K, Ewenstein B, Golan D, Pober J. Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells. Journal Of Cellular Physiology 1994, 160: 545-554. PMID: 7521337, DOI: 10.1002/jcp.1041600318.Peer-Reviewed Original ResearchConceptsEndothelial cellsAgonist concentrationsSingle human umbilical vein endothelial cellCytoplasmic free calcium ionsNumber of cellsPeak elevationHigh agonist concentrationsCytoplasmic free calciumHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVWF secretionIndividual endothelial cellsAmount of secretionVein endothelial cellsHuman endothelial cellsAgonist treatmentVon Willebrand factor secretionAgonist doseExtracellular Ca2Maximal secretionP-selectinThrombin receptorHigh dosesIntracellular storesFactor secretion
1992
Calcium/calmodulin transduces thrombin-stimulated secretion: studies in intact and minimally permeabilized human umbilical vein endothelial cells.
Birch KA, Pober JS, Zavoico GB, Means AR, Ewenstein BM. Calcium/calmodulin transduces thrombin-stimulated secretion: studies in intact and minimally permeabilized human umbilical vein endothelial cells. Journal Of Cell Biology 1992, 118: 1501-1510. PMID: 1522120, PMCID: PMC2289613, DOI: 10.1083/jcb.118.6.1501.Peer-Reviewed Original ResearchConceptsVon Willebrand factorEndothelial cellsProtein kinase CCell-permeant calcium chelatorExtracellular calcium chelator EGTAVWF secretionAgonist-induced elevationInhibitory peptidesSignal transduction pathwaysHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsCultured endothelial cellsCalcium chelator EGTAPermeabilized human umbilical vein endothelial cellsVein endothelial cellsHuman endothelial cellsPhorbol esterRole of calmodulinSecond messenger pathwaysIntracellular calciumIntact human endothelial cellsTransduction pathwaysThrombin receptorCalcium ionophoreLarge intracellular proteinThrombin and histamine rapidly stimulate the phosphorylation of the myristoylated alanine‐rich C‐kinase substrate in human umbilical vein endothelial cells: Evidence for distinct patterns of protein kinase activation
Jacobson B, Pober J, Fenton J, Ewenstein B. Thrombin and histamine rapidly stimulate the phosphorylation of the myristoylated alanine‐rich C‐kinase substrate in human umbilical vein endothelial cells: Evidence for distinct patterns of protein kinase activation. Journal Of Cellular Physiology 1992, 152: 166-176. PMID: 1320036, DOI: 10.1002/jcp.1041520121.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedDose-Response Relationship, DrugElectrophoresis, Polyacrylamide GelEndothelium, VascularEnzyme ActivationHistamineHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMolecular WeightMyristoylated Alanine-Rich C Kinase SubstratePhosphorylationPrecipitin TestsProtein KinasesProteinsReceptors, Cell SurfaceThrombin
1989
IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis.
Zavoico GB, Ewenstein BM, Schafer AI, Pober JS. IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis. The Journal Of Immunology 1989, 142: 3993-9. PMID: 2497185, DOI: 10.4049/jimmunol.142.11.3993.Peer-Reviewed Original ResearchMeSH KeywordsArachidonic AcidArachidonic AcidsCells, CulturedChromatography, Thin LayerCycloheximideDrug SynergismEndothelium, VascularEpoprostenolHistamineHumansInterleukin-1Platelet Activating FactorProstaglandin Endoperoxides, SyntheticProstaglandin H2Prostaglandins HThrombinVon Willebrand Factor