1998
Tumor necrosis factor is delivered to mitochondria where a tumor necrosis factor-binding protein is localized.
Ledgerwood EC, Prins JB, Bright NA, Johnson DR, Wolfreys K, Pober JS, O'Rahilly S, Bradley JR. Tumor necrosis factor is delivered to mitochondria where a tumor necrosis factor-binding protein is localized. Laboratory Investigation 1998, 78: 1583-9. PMID: 9881958.Peer-Reviewed Original ResearchMeSH KeywordsAdipocytesAntibodies, MonoclonalCarrier ProteinsCells, CulturedHumansImmunohistochemistryIntracellular MembranesIodine RadioisotopesLysosomesMicroscopy, ImmunoelectronMitochondriaReceptors, Tumor Necrosis FactorReceptors, Tumor Necrosis Factor, Type ITumor Necrosis Factor Decoy ReceptorsTumor Necrosis Factor-alphaU937 CellsConceptsInner mitochondrial membraneExtracellular ligandsMitochondrial membraneTumor necrosis factor receptorIsolated mitochondriaBinding proteinNecrosis factor receptorMitochondriaCell surfaceImmunoelectron microscopyProteinFactor receptorSubcellular fractionsWestern blottingFactor binding proteinTNF effectsPathwayDiverse actionsExogenous TNFTNFR-IIMonoclonal antibodiesTumor necrosis factor binding proteinLysosomesTumor necrosis factorBlotting
1994
Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28
Murray A, Khodedoust M, Pober J, Bothwell A. Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28. Immunity 1994, 1: 57-63. PMID: 7889399, DOI: 10.1016/1074-7613(94)90009-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationAntigens, HeterophileCD2 AntigensCD28 AntigensCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansIn Vitro TechniquesLigandsLymphocyte ActivationSpecies SpecificitySwineT-LymphocytesConceptsPorcine aortic endothelial cellsHuman umbilical vein endothelial cellsHuman T cellsCTLA-4-IgT cellsAortic endothelial cellsEndothelial cellsCultured porcine aortic endothelial cellsMHC class II moleculesClass II moleculesMHC class IUmbilical vein endothelial cellsVein endothelial cellsAllogeneic responseMHC antigensHuman CD8CD28 pathwayCD8 moleculesHuman CD4Species-specific monoclonal antibodiesCD2 pathwayMonoclonal antibodiesClass IDirect presentationCD4
1993
Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration.
Slowik MR, De Luca LG, Fiers W, Pober JS. Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration. American Journal Of Pathology 1993, 143: 1724-30. PMID: 7504889, PMCID: PMC1887273.Peer-Reviewed Original ResearchConceptsEndothelial leukocyte adhesion molecule-1Endothelial cellsHuman endothelial cellsTumor necrosis factor concentrationsMonoclonal antibodiesNecrosis factor concentrationsLeukocyte adhesion molecule-1Major histocompatibility complex moleculesActivation of ECTumor necrosis factorAdhesion molecule-1Class I major histocompatibility complex moleculesLeukocyte adhesion moleculesTumor necrosis factor receptorELAM-1 inductionRecombinant human TNFHistocompatibility complex moleculesNecrosis factor receptorCultured endothelial cellsP75 receptorTumor necrosisNecrosis factorMolecule-1Receptor typesTNF receptor
1991
MS-1 sinusoidal endothelial antigen is expressed by factor XIIIa+, HLA-DR+ dermal perivascular dendritic cells.
Walsh LJ, Goerdt S, Pober JS, Sueki H, Murphy GF. MS-1 sinusoidal endothelial antigen is expressed by factor XIIIa+, HLA-DR+ dermal perivascular dendritic cells. Laboratory Investigation 1991, 65: 732-41. PMID: 1684403.Peer-Reviewed Original ResearchConceptsPerivascular dendritic cellsDendritic cellsEndothelial cellsInterstitial cellsDendritic interstitial cellsNormal human skinVascular endothelial cellsNonlymphoid organsEndothelial antigensHLA-DRDermal dendrocytesMast cellsImmunofluorescence labeling techniquePerivascular localizationSinusoidal endotheliumPhagocytic macrophagesPerivascular cellsSinusoidal spacesLymphatic endothelial cellsMonoclonal antibodiesBlood vascular endothelial cellsAntibodiesFactor XIIIaBasement membraneDendritic morphology
1987
Identification of an inducible endothelial-leukocyte adhesion molecule.
Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA. Identification of an inducible endothelial-leukocyte adhesion molecule. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 9238-9242. PMID: 2827173, PMCID: PMC299728, DOI: 10.1073/pnas.84.24.9238.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell surface proteinsEndothelial cell surface proteinsUnstimulated human endothelial cellsCell surface structuresHL-60 cellsSame polypeptideHL-60 adhesionCultured human endothelial cellsAdhesion assaysAdhesive interactionsH18/7Adhesion moleculesCytokine-stimulated human endothelial cellsDependent fashionCell surface antigensEndothelial cellsEndothelial leukocyte adhesion moleculeEndothelial leukocyte adhesion molecule-1AdhesionCellsAdhesion molecule-1Monoclonal antibodiesSites of inflammationLesser extent
1986
Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon.
Pober JS, Gimbrone MA, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. The Journal Of Immunology 1986, 137: 1893-6. PMID: 3091693, DOI: 10.4049/jimmunol.137.6.1893.Peer-Reviewed Original ResearchConceptsTumor necrosis factorIFN-gammaHuman umbilical vein endothelialNecrosis factorIL-1Interleukin-1HUVE cellsImmune interferonIntercellular adhesion molecule-1Endothelial cellsDifferent immune mediatorsAdhesion molecule-1Murine monoclonal antibodiesHuman endothelial cellsImmune mediatorsPattern of activationActivation antigensAntigenic modulationB antigensDifferent lymphokinesMolecule-1Monoclonal antibodiesProgressive increaseInterferonAntigen
1983
Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon.
Pober JS, Gimbrone MA, Cotran RS, Reiss CS, Burakoff SJ, Fiers W, Ault KA. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. Journal Of Experimental Medicine 1983, 157: 1339-1353. PMID: 6403654, PMCID: PMC2186979, DOI: 10.1084/jem.157.4.1339.Peer-Reviewed Original ResearchConceptsAllogeneic T cellsT cellsIa antigensEndothelial cellsPeripheral blood mononuclear cellsBlood mononuclear cellsCultured human umbilical vein endothelial cellsExpression of IaActivated T cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsAllograft rejectionVein endothelial cellsGamma interferon geneFluorescence flow cytometryHuman gamma interferonMononuclear cellsImmune responseGamma interferonVascular endotheliumIa expressionFlow cytometryImmune interferonMonoclonal antibodiesAntigen
1982
Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation.
Pober JS, Gimbrone MA. Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6641-6645. PMID: 6815654, PMCID: PMC347184, DOI: 10.1073/pnas.79.21.6641.Peer-Reviewed Original ResearchConceptsHuman vascular endothelial cellsCell shape changesVascular endothelial cellsEndothelial cellsMembrane proteinsCell divisionRadioiodinated membrane proteinsHuman endothelial cellsStandard culture conditionsIntact cellsCultured human endothelial cellsBiochemical demonstrationImmunoprecipitationCulture conditionsConfluent culturesExpressionMonoclonal antibody bindingCellsPrimary culturesShape changesMonoclonal antibodiesLectin phytohemagglutininProteinRegulationImportant implications