1982
Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation.
Pober JS, Gimbrone MA. Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6641-6645. PMID: 6815654, PMCID: PMC347184, DOI: 10.1073/pnas.79.21.6641.Peer-Reviewed Original ResearchConceptsHuman vascular endothelial cellsCell shape changesVascular endothelial cellsEndothelial cellsMembrane proteinsCell divisionRadioiodinated membrane proteinsHuman endothelial cellsStandard culture conditionsIntact cellsCultured human endothelial cellsBiochemical demonstrationImmunoprecipitationCulture conditionsConfluent culturesExpressionMonoclonal antibody bindingCellsPrimary culturesShape changesMonoclonal antibodiesLectin phytohemagglutininProteinRegulationImportant implications[34] Proteolysis of rhodopsin
Pober J. [34] Proteolysis of rhodopsin. Methods In Enzymology 1982, 81: 236-239. PMID: 7047990, DOI: 10.1016/s0076-6879(82)81036-7.Peer-Reviewed Original ResearchConceptsLight-activated enzymesOrganization of rhodopsinMembrane proteinsPolypeptide sequenceHigh-resolution mappingConformational changesLimited proteolysisBovine rhodopsinCleavage siteRod outer segmentsRhodopsinProteolysisRegion of interactionDisk membranesResolution mappingSpecific sitesOuter segmentsValuable probe
1979
Selective labelling of the hydrophobic segments of intrinsic membrane proteins with a lipophilic photogenerated carbene
GOLDMAN D, POBER J, WHITE J, BAYLEY H. Selective labelling of the hydrophobic segments of intrinsic membrane proteins with a lipophilic photogenerated carbene. Nature 1979, 280: 841-843. PMID: 471056, DOI: 10.1038/280841a0.Peer-Reviewed Original ResearchConceptsIntrinsic membrane proteinsMembrane proteinsLipid bilayersCarbon-hydrogen bondsMembrane-associated regionsHydrophobic amino acidsProtein segmentsAryl azidesCarbeneHydrophobic peptidesReactive speciesAryl nitrenesBiological membranesNitreneAmino acidsHydrophobic segmentsHydrocarbon regionProteinGlycophorin ASelective labelingBilayersReagentsLipophilicReactivityAttractive candidate