2022
Monobody adapter for functional antibody display on nanoparticles for adaptable targeted delivery applications
Albert C, Bracaglia L, Koide A, DiRito J, Lysyy T, Harkins L, Edwards C, Richfield O, Grundler J, Zhou K, Denbaum E, Ketavarapu G, Hattori T, Perincheri S, Langford J, Feizi A, Haakinson D, Hosgood SA, Nicholson ML, Pober JS, Saltzman WM, Koide S, Tietjen GT. Monobody adapter for functional antibody display on nanoparticles for adaptable targeted delivery applications. Nature Communications 2022, 13: 5998. PMID: 36220817, PMCID: PMC9553936, DOI: 10.1038/s41467-022-33490-8.Peer-Reviewed Original ResearchConceptsTargeted delivery applicationsTargeted nanoparticlesAntibody immobilizationAntigen-binding functionNanoparticlesNP surfaceDelivery applicationsRobust deliveryEndothelial cellsAmine couplingAntibody displayClinical translationIntracellular drug levelsVascular endothelial cellsCultured endothelial cellsNanomedicineDrug levelsVivo perfusionOrgan transplantsAdapterClinical settingHuman kidneyHuman settingImmobilizationEfficacy
2020
Lysis of cold‐storage‐induced microvascular obstructions for ex vivo revitalization of marginal human kidneys
DiRito JR, Hosgood SA, Reschke M, Albert C, Bracaglia LG, Ferdinand JR, Stewart BJ, Edwards CM, Vaish AG, Thiru S, Mulligan DC, Haakinson DJ, Clatworthy MR, Saltzman WM, Pober JS, Nicholson ML, Tietjen GT. Lysis of cold‐storage‐induced microvascular obstructions for ex vivo revitalization of marginal human kidneys. American Journal Of Transplantation 2020, 21: 161-173. PMID: 32627324, PMCID: PMC7775334, DOI: 10.1111/ajt.16148.Peer-Reviewed Original ResearchConceptsNormothermic machine perfusionHuman kidneyMicrovascular obstructionMarginal organsHigh-risk donorsSevere organ shortageLikelihood of complicationsMicrovascular blood flowAccumulation of fibrinogenCold storage injuryTissue plasminogen activatorImmediate translational potentialMicrovascular pluggingRenal functionRenal injuryMachine perfusionTubular epitheliumBlood flowIntravascular releaseOrgan shortagePhysiologic impactKidneyTranslational potentialPlasminogen activatorVivo assessment
2001
Expression of Tumor Necrosis Factor Receptors in Normal Kidney and Rejecting Renal Transplants
Al-Lamki R, Wang J, Skepper J, Thiru S, Pober J, Bradley J. Expression of Tumor Necrosis Factor Receptors in Normal Kidney and Rejecting Renal Transplants. Laboratory Investigation 2001, 81: 1503-1515. PMID: 11706058, DOI: 10.1038/labinvest.3780364.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAntigens, CDColoring AgentsEosine Yellowish-(YS)Fluorescent DyesGene ExpressionGraft RejectionHematoxylinHumansKidneyKidney Failure, ChronicKidney TransplantationMicroscopy, ImmunoelectronReceptors, Tumor Necrosis FactorReceptors, Tumor Necrosis Factor, Type IReceptors, Tumor Necrosis Factor, Type IITumor Necrosis Factor-alphaConceptsDistal convoluted tubuleNormal kidneyTubular epithelial cellsTNFR-1Renal transplantEpithelial cellsTNFR-2Acute cellular rejectionTNFR-1 expressionAcute transplant rejectionTumor necrosis factor receptorEndothelium of glomeruliNecrosis factor receptorInteraction of TNFAcute rejectionCellular rejectionTNFR expressionTransplant rejectionCultured cellsConvoluted tubulesRenal kidneyTNF receptorTNFKidneyHuman kidney