2023
IL-6 trans-signaling in a humanized mouse model of scleroderma
Odell I, Agrawal K, Sefik E, Odell A, Caves E, Kirkiles-Smith N, Horsley V, Hinchcliff M, Pober J, Kluger Y, Flavell R. IL-6 trans-signaling in a humanized mouse model of scleroderma. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2306965120. PMID: 37669366, PMCID: PMC10500188, DOI: 10.1073/pnas.2306965120.Peer-Reviewed Original ResearchConceptsBone marrow-derived immune cellsIL-6Human hematopoietic stem cellsImmune cellsT cellsScleroderma skinSoluble IL-6 receptorCD8 T cellsHumanized mouse modelPathogenesis of sclerodermaMesenchymal cellsFibroblast-derived IL-6IL-6 receptorIL-6 signalingT cell activationHuman IL-6Human T cellsExpression of collagenFibrosis improvementPansclerotic morpheaHuman endothelial cellsHumanized miceReduced markersSkin graftsHuman CD4
2013
Alloantibody and Complement Promote T Cell–Mediated Cardiac Allograft Vasculopathy Through Noncanonical Nuclear Factor-&kgr;B Signaling in Endothelial Cells
Jane-wit D, Manes TD, Yi T, Qin L, Clark P, Kirkiles-Smith NC, Abrahimi P, Devalliere J, Moeckel G, Kulkarni S, Tellides G, Pober JS. Alloantibody and Complement Promote T Cell–Mediated Cardiac Allograft Vasculopathy Through Noncanonical Nuclear Factor-&kgr;B Signaling in Endothelial Cells. Circulation 2013, 128: 2504-2516. PMID: 24045046, PMCID: PMC3885874, DOI: 10.1161/circulationaha.113.002972.Peer-Reviewed Original ResearchConceptsCardiac allograft vasculopathyPanel reactive antibodyNuclear factor-κB signalingFactor-κB signalingAllograft vasculopathyT cellsEndothelial cellsMembrane attack complexAlloreactive T cell activationChronic antibody-mediated rejectionNoncanonical nuclear factorProinflammatory gene programAntibody-mediated rejectionDonor-specific antibodiesGraft endothelial cellsLate allograft lossAlloreactive T cellsAllogeneic endothelial cellsT cell activationAttack complexHuman T cellsAllograft lossHeart transplantationTransplantation patientsLesion pathogenesis
2011
Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells
Lebastchi AH, Khan SF, Qin L, Li W, Zhou J, Hibino N, Yi T, Rao DA, Pober JS, Tellides G. Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells. American Journal Of Transplantation 2011, 11: 2332-2341. PMID: 21812925, PMCID: PMC3203343, DOI: 10.1111/j.1600-6143.2011.03676.x.Peer-Reviewed Original ResearchConceptsEffector T cellsMemory T cellsNaïve T cellsT cellsIL-17Vascular cellsEffector memory T cellsAlloreactive memory T cellsGrowth factor-beta expressionMedial smooth muscle cellsHuman effector memory T cellsHuman coronary artery graftsIL-17 productionCoronary artery graftsIFN-γ productionAllogeneic endothelial cellsInterferon-gamma productionDevelopment of arteriosclerosisExpression of TGFRole of TGFSmooth muscle cellsImmunodeficient mouse hostsHuman T cellsIntimal compartmentMedial infiltration
2009
Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death
Choy JC, Pober JS. Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death. American Journal Of Transplantation 2009, 9: 2281-2291. PMID: 19663890, PMCID: PMC3505447, DOI: 10.1111/j.1600-6143.2009.02771.x.Peer-Reviewed Original ResearchConceptsHuman CD8 T cellsCD8 T cellsInducible NO synthaseT cellsActivated T cellsNitric oxideDeprivation-induced cell deathCell deathAllogeneic endothelial cellsT cell proliferationActivation-induced cell deathCytokine deprivationGeneration of NOT cell deathExogenous nitric oxideHuman T cellsNO augmentsAllogeneic responseS-nitrosylationNO synthaseProtective effectActivity of caspasesEndothelial cellsPharmacological inhibitorsDeathParacrine Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death (141.3)
Choy J, Pober J. Paracrine Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death (141.3). The Journal Of Immunology 2009, 182: 141.3-141.3. DOI: 10.4049/jimmunol.182.supp.141.3.Peer-Reviewed Original ResearchHuman CD8 T cellsCD8 T cellsT cellsActivated T cellsDeprivation-induced cell deathCell deathAllogeneic endothelial cellsT cell proliferationActivation-induced cell deathCytokine deprivationHuman T cellsProtein expression levelsNO augmentsAllogeneic responseS-nitrosylationProtective effectExogenous NOActivity of caspasesEndothelial cellsPharmacological inhibitorsDeathBcl-2Cell proliferationExpression levelsINOS
1996
Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells CONVERGENCE OF COSTIMULATORY SIGNALS AND DIFFERENCES FROM TRANSFORMED T CELLS (∗)
Hughes C, Pober J. Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells CONVERGENCE OF COSTIMULATORY SIGNALS AND DIFFERENCES FROM TRANSFORMED T CELLS (∗). Journal Of Biological Chemistry 1996, 271: 5369-5377. PMID: 8621390, DOI: 10.1074/jbc.271.10.5369.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesB-LymphocytesCD3 ComplexCell Line, TransformedCell NucleusCells, CulturedFlow CytometryGene Expression RegulationHumansInterleukin-2KineticsLuciferasesLymphocyte ActivationMolecular Sequence DataNF-kappa BPromoter Regions, GeneticRecombinant ProteinsRegulatory Sequences, Nucleic AcidSignal TransductionT-LymphocytesTranscription FactorsTranscription, GeneticTransfectionTumor Cells, CulturedConceptsNormal T cellsT cellsCostimulatory signalsDifferent costimulatory signalsT cell receptorActivated T-cells (NFAT) sitesNormal human T cellsHuman T cellsT cell sitesTransformed T cellsCD2 antibodiesNF-kappaB siteAccessory cellsTumor cell linesCell receptorInterleukin-2 geneNuclear factorPrimary activationIL-2 promoterJurkat T cellsProximal AP-1 siteCell linesAntibodiesAP-1 siteTranscriptional regulation
1994
Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28
Murray A, Khodedoust M, Pober J, Bothwell A. Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28. Immunity 1994, 1: 57-63. PMID: 7889399, DOI: 10.1016/1074-7613(94)90009-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationAntigens, HeterophileCD2 AntigensCD28 AntigensCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansIn Vitro TechniquesLigandsLymphocyte ActivationSpecies SpecificitySwineT-LymphocytesConceptsPorcine aortic endothelial cellsHuman umbilical vein endothelial cellsHuman T cellsCTLA-4-IgT cellsAortic endothelial cellsEndothelial cellsCultured porcine aortic endothelial cellsMHC class II moleculesClass II moleculesMHC class IUmbilical vein endothelial cellsVein endothelial cellsAllogeneic responseMHC antigensHuman CD8CD28 pathwayCD8 moleculesHuman CD4Species-specific monoclonal antibodiesCD2 pathwayMonoclonal antibodiesClass IDirect presentationCD4
1983
Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon
Pober J, Collins T, Gimbrone M, Cotran R, Gitlin J, Fiers W, Clayberger C, Krensky A, Burakoff S, Reiss C. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature 1983, 305: 726-729. PMID: 6415484, DOI: 10.1038/305726a0.Peer-Reviewed Original ResearchConceptsCytolytic T lymphocytesRecombinant immune interferonIa antigensT cellsIa expressionAlloreactive cytolytic T lymphocytesClass II major histocompatibility complex antigensT-lymphocyte-mediated responsesImmune interferonEndothelial cellsMajor histocompatibility complex antigensForeign class IHistocompatibility complex antigensHuman vascular endothelialHuman umbilical vein endothelial cellsHUVE cellsCapillary endothelial cellsUmbilical vein endothelial cellsHuman T cellsImmunological cell typesAllograft rejectionVein endothelial cellsOrgan graftsT lymphocytesComplex antigens