2013
Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation
Devalliere J, Chang WG, Andrejecsk JW, Abrahimi P, Cheng CJ, Jane‐wit D, Saltzman WM, Pober JS. Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation. The FASEB Journal 2013, 28: 908-922. PMID: 24221087, PMCID: PMC3898640, DOI: 10.1096/fj.13-238527.Peer-Reviewed Original ResearchConceptsHuman umbilical vein ECsEndothelial cellsMiR-132MicroRNA-132Cultured human umbilical vein endothelial cellsNumber of microvesselsGrowth factor-induced proliferationHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsEndothelial cell transplantationCultured endothelial cellsEndogenous growth factorsEC transplantationVein endothelial cellsCell transplantationImmunodeficient miceTissue perfusionTransplantationMiR deliveryGrowth factorIntegrin αvβ3Endocytosed nanoparticlesSquare millimeterBiological effectsControl transfectionPericytes modulate endothelial sprouting
Chang WG, Andrejecsk JW, Kluger MS, Saltzman WM, Pober JS. Pericytes modulate endothelial sprouting. Cardiovascular Research 2013, 100: 492-500. PMID: 24042014, PMCID: PMC3826704, DOI: 10.1093/cvr/cvt215.Peer-Reviewed Original ResearchMeSH KeywordsBecaplerminCoculture TechniquesCulture Media, ConditionedHepatocyte Growth FactorHuman Umbilical Vein Endothelial CellsHumansMicrovesselsNeovascularization, PhysiologicParacrine CommunicationPericytesProto-Oncogene Proteins c-bcl-2Proto-Oncogene Proteins c-sisSignal TransductionSpheroids, CellularTime FactorsTransfectionVascular Endothelial Growth Factor AParacrine exchanges of molecular signals between alginate-encapsulated pericytes and freely suspended endothelial cells within a 3D protein gel
Andrejecsk JW, Cui J, Chang WG, Devalliere J, Pober JS, Saltzman WM. Paracrine exchanges of molecular signals between alginate-encapsulated pericytes and freely suspended endothelial cells within a 3D protein gel. Biomaterials 2013, 34: 8899-8908. PMID: 23973174, PMCID: PMC3839675, DOI: 10.1016/j.biomaterials.2013.08.008.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsParacrine signalsFunctioning of tissuesProper survivalEndothelial cellsUmbilical vein endothelial cellsMolecular signalsRegulated deliveryVein endothelial cellsVessel-like structuresLiving cellsProtein gelsHepatocyte growth factorTherapeutic proteinsParacrine exchangesGrowth factorMicrovascular pericytesProteinAngiogenic proteinsCellsVascular tissue engineeringHUVEC behaviorTissue constructsPericytesLocal environment
2009
Engineered Molecular Delivery for Control and Enhancement of Transplanted Endothelial Cell Fate in Tissue Engineering
Jay S, Shepherd B, Andrejecsk J, Kyriakides T, Pober J, Saltzman W. Engineered Molecular Delivery for Control and Enhancement of Transplanted Endothelial Cell Fate in Tissue Engineering. 2009, 1: 1-2. DOI: 10.1109/nebc.2009.4967788.Peer-Reviewed Original ResearchTissue engineeringAdvanced tissue engineeringMultifunctional hydrogelsTissue constructsFunctional microvascular networksTransplantation of ECsEndothelial cell fateBiomaterial strategiesAlginate microparticlesVascular endothelial growth factorEndothelial cellsEngineeringHydrogelsCell fateGrowth factorSustained releaseProtein 1Mural cellsMolecular deliveryMonocyte chemoattractant protein-1Cell populationsProtein therapeuticsProtein matrixAngiogenic growth factorsProtein delivery
2000
A Phosphatidylinositol 3-Kinase/Akt Pathway, Activated by Tumor Necrosis Factor or Interleukin-1, Inhibits Apoptosis but Does Not Activate NFκB in Human Endothelial Cells*
Madge L, Pober J. A Phosphatidylinositol 3-Kinase/Akt Pathway, Activated by Tumor Necrosis Factor or Interleukin-1, Inhibits Apoptosis but Does Not Activate NFκB in Human Endothelial Cells*. Journal Of Biological Chemistry 2000, 275: 15458-15465. PMID: 10748004, DOI: 10.1074/jbc.m001237200.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesApoptosisCells, CulturedChromonesCulture Media, Serum-FreeEndothelium, VascularEnzyme ActivationEnzyme InhibitorsHumansInterleukin-1KineticsMorpholinesNF-kappa BPhosphatidylinositol 3-KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRecombinant ProteinsTumor Necrosis Factor-alphaUmbilical VeinsWortmanninConceptsHuman endothelial cellsProtein kinaseStress-activated protein kinaseAkt pathwayMitogen-activated protein kinaseProtein kinase AktPromoter-reporter geneInhibitor of phosphatidylinositolEndothelial cellsSerum-deprived endothelial cellsPhosphorylation of AktGrowth factorAnti-apoptotic pathwaysKinase AktGene productsAnti-apoptotic effectsInhibits ApoptosisSerum deprivationAktLY294002Phospho-AktPro-inflammatory gene productsTranscriptionKinasePhosphatidylinositolControl of Apoptosis during Angiogenesis by Survivin Expression in Endothelial Cells
O'Connor D, Schechner J, Adida C, Mesri M, Rothermel A, Li F, Nath A, Pober J, Altieri D. Control of Apoptosis during Angiogenesis by Survivin Expression in Endothelial Cells. American Journal Of Pathology 2000, 156: 393-398. PMID: 10666367, PMCID: PMC1850029, DOI: 10.1016/s0002-9440(10)64742-6.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCell DivisionCell SurvivalCells, CulturedEndothelial Growth FactorsEndothelium, VascularFibroblast Growth Factor 2HumansInhibitor of Apoptosis ProteinsLymphokinesMicrotubule-Associated ProteinsMitogensNeoplasm ProteinsNeovascularization, PhysiologicProteinsSurvivinVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsTumor necrosis factor alpha/cycloheximideControl of apoptosisEndothelial cellsSurvivin expressionQuiescent endothelial cellsEndothelial cell survivalGrowth factorApoptosis inhibitor survivinTwo-dimensional cultureCaspase-3 activityRecombinant expressionFibroblast growth factorBasic fibroblast growth factorCell survivalStrong inductionProtein survivinPathological angiogenesisProtective genesRNA expressionVascular tubesSurvivinExpressionVascular endothelial growth factorApoptosisCells
1995
IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro
Petzelbauer P, Watson C, Watson C, Pfau S, Pober J. IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro. Cytokine 1995, 7: 267-272. PMID: 7543779, DOI: 10.1006/cyto.1995.0031.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsIL-8Cultured human umbilical vein endothelial cellsDermal microvascular endothelial cellsEndothelial cellsHuman endothelial cellsRecombinant human IL-8IL-8 treatmentMicrovascular human endothelial cellsReceptor mRNA expressionEndothelial cell growth factorHuman IL-8Human dermal microvascular endothelial cellsMicrovascular endothelial cellsCytoplasmic calcium concentrationUmbilical vein endothelial cellsCell growth factorVein endothelial cellsAngiogenic factorsMRNA expressionDirect actionGrowth factorCalcium concentrationIndirect actionProliferative rate