2021
Coronavirus Disease 2019 (COVID-19) Coronary Vascular Thrombosis Correlation with Neutrophil but Not Endothelial Activation
Johnson JE, McGuone D, Xu ML, Jane-Wit D, Mitchell RN, Libby P, Pober JS. Coronavirus Disease 2019 (COVID-19) Coronary Vascular Thrombosis Correlation with Neutrophil but Not Endothelial Activation. American Journal Of Pathology 2021, 192: 112-120. PMID: 34599881, PMCID: PMC8479934, DOI: 10.1016/j.ajpath.2021.09.004.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Intracellular adhesion molecule-1Adhesion molecule-1Von Willebrand factorEndothelial activationMolecule-1Severe coronavirus disease 2019Neutrophil extracellular trap formationCell adhesion molecule-1COVID-19 cohortCOVID-19 patientsNeutrophil-platelet aggregatesCoronavirus disease 2019Extracellular trap formationCOVID-19Transcription factor p65Extensive thrombosisLymphocytic infiltrationMyocardial injuryThrombotic diathesisInflammatory activationNeutrophil activationCardiovascular diseaseDisease 2019Autopsy tissue
2014
Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2
Wang C, Qin L, Manes TD, Kirkiles-Smith NC, Tellides G, Pober JS. Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2. Journal Of Experimental Medicine 2014, 211: 395-404. PMID: 24516119, PMCID: PMC3949571, DOI: 10.1084/jem.20131125.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceBlotting, WesternCell AdhesionChromatin ImmunoprecipitationDNA PrimersEndothelial CellsFlow CytometryHumansImmunoblottingMechanistic Target of Rapamycin Complex 2Microscopy, FluorescenceMultiprotein ComplexesOncogene Protein v-aktReal-Time Polymerase Chain ReactionSirolimusT-LymphocytesTOR Serine-Threonine KinasesTumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1VCAM-1 expressionEndothelial cellsActivation of ERK1/2Cell adhesion molecule-1TNF inductionInfiltration of leukocytesAdhesion molecule-1Inhibition of TNFPotential therapeutic targetAbility of rapamycinAbility of TNFTranscription factor IRF-1Hyperactivation of ERK1/2Inhibition of ERK1/2Venular flowT cellsEndothelial expressionInflamed tissuesVascular endotheliumMolecule-1Therapeutic targetRapamycin pretreatmentRenal glomeruliTNF
2013
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment
Lauridsen HM, Pober JS, Gonzalez AL. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment. The FASEB Journal 2013, 28: 1166-1180. PMID: 24297702, PMCID: PMC3929680, DOI: 10.1096/fj.13-240986.Peer-Reviewed Original ResearchConceptsAdhesion molecule-1Cell adhesion molecule-1Molecule-1Endothelial cellsPostcapillary venulesBasement membraneVascular cell adhesion molecule-1Intercellular adhesion molecule-1Tumor necrosis factor αMicrovascular leukocyte recruitmentNecrosis factor αLate antigen-4Platelet endothelial cell adhesion molecule-1TNF-α activationInflammatory cascadeAntigen-4Neutrophil extravasationInterleukin-8Leukocyte recruitmentNeutrophil adhesionFactor αTNFPericytesVenular shear stressAnti-CD99
2001
HUMAN T CELLS INFILTRATE AND INJURE PIG CORONARY ARTERY GRAFTS WITH ACTIVATED BUT NOT QUIESCENT ENDOTHELIUM IN IMMUNODEFICIENT MOUSE HOSTS1
Tereb D, Kirkiles-Smith N, Kim R, Wang Y, Rudic R, Schechner J, Lorber M, Bothwell A, Pober J, Tellides G. HUMAN T CELLS INFILTRATE AND INJURE PIG CORONARY ARTERY GRAFTS WITH ACTIVATED BUT NOT QUIESCENT ENDOTHELIUM IN IMMUNODEFICIENT MOUSE HOSTS1. Transplantation 2001, 71: 1622-1630. PMID: 11435975, DOI: 10.1097/00007890-200106150-00023.Peer-Reviewed Original ResearchConceptsTumor necrosis factorHuman tumor necrosis factorArtery graftT cellsNecrosis factorPig coronary artery endothelial cellsHuman T-cell infiltrationVascular cell adhesion molecule-1Human peripheral blood mononuclearHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsHuman leukocytesEndothelial cellsMajor histocompatibility complex antigensCell adhesion molecule-1Graft endothelial cellsT-cell infiltratesBeige mouse modelCoronary artery endothelial cellsT cell infiltrationCoronary artery graftsBlood mononuclear cellsPeripheral blood mononuclearCoronary artery segmentsAdhesion molecule-1
1996
Prolonged cytokine exposure causes a dynamic redistribution of endothelial cell adhesion molecules to intercellular junctions.
Bradley JR, Pober JS. Prolonged cytokine exposure causes a dynamic redistribution of endothelial cell adhesion molecules to intercellular junctions. Laboratory Investigation 1996, 75: 463-72. PMID: 8874379.Peer-Reviewed Original ResearchConceptsCell junctionsProtein redistributionMembrane protein redistributionLongitudinal stress fibersDynamic redistributionIntercellular adhesion molecule-2Cultured umbilical vein endothelial cellsBeta 3 integrinAdhesion moleculesBeta 1 integrinCell adhesion moleculeDense peripheral bandStress fibersCytoskeletal actinEndothelial cellsUmbilical vein endothelial cellsActin filamentsCell adhesion molecule-1Vein endothelial cellsAdhesion molecule-1Intercellular adhesion molecule 1 moleculesIntercellular junctionsApical surfaceVascular cell adhesion molecule-1Peripheral bandIL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma c chain.
Palmer-Crocker RL, Hughes CC, Pober JS. IL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma c chain. Journal Of Clinical Investigation 1996, 98: 604-609. PMID: 8698849, PMCID: PMC507467, DOI: 10.1172/jci118829.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCells, CulturedEndothelium, VascularHumansInterleukin-3Interleukin-4Janus Kinase 2Molecular Sequence DataPhosphorylationProtein-Tyrosine KinasesProto-Oncogene ProteinsReceptors, Interleukin-2RNA, MessengerSTAT6 Transcription FactorTrans-ActivatorsTyrosineVascular Cell Adhesion Molecule-1ConceptsIL-4 responsesIL-13IL-4Human endothelial cellsEndothelial cellsVascular cell adhesion molecule-1Cell adhesion molecule-1Cultured human vascular endothelial cellsAdhesion molecule-1IL-15 receptorTyrosine kinaseCommon signaling subunitHuman vascular endothelial cellsJAK2 tyrosine kinaseVascular endothelial cellsSTAT6 transcription factorReverse transcription-PCR methodGamma c chainTranscription-PCR methodGC-independent pathwayIL-2IL-9IL-7IL-4RMolecule-1A Sustained Reduction in IκB-β May Contribute to Persistent NF-κB Activation in Human Endothelial Cells*
Johnson D, Douglas I, Jahnke A, Ghosh S, Pober J. A Sustained Reduction in IκB-β May Contribute to Persistent NF-κB Activation in Human Endothelial Cells*. Journal Of Biological Chemistry 1996, 271: 16317-16322. PMID: 8663191, DOI: 10.1074/jbc.271.27.16317.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCell Adhesion MoleculesCell MembraneCell NucleusCells, CulturedConsensus SequenceCytosolDNADNA-Binding ProteinsEndothelium, VascularGene ExpressionHistocompatibility Antigens Class IHumansI-kappa B ProteinsIntercellular Adhesion Molecule-1Interleukin-1KineticsMolecular Sequence DataNF-kappa BOligodeoxyribonucleotidesTetradecanoylphorbol AcetateTumor Necrosis Factor-alphaUmbilical VeinsVascular Cell Adhesion Molecule-1ConceptsAdhesion molecule-1Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Phorbol myristate acetateInterleukin-1alphaNF-kappaB activationMolecule-1Sustained reductionNF-kappaBEndothelial cellsIkappaB-alphaIkappaB-betaHuman endothelial cellsHuman leukocyte antigen (HLA) class INF-kappaB.Inhibitory protein IkappaB-alphaCell surface molecule expressionIkappaB-beta degradationPersistent NF-κB activationSurface molecule expressionAntigen class INF-κB activationIkappaB-beta levelsDe novo expressionActivation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1.
Karmann K, Min W, Fanslow WC, Pober JS. Activation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1. Journal Of Experimental Medicine 1996, 184: 173-182. PMID: 8691131, PMCID: PMC2192678, DOI: 10.1084/jem.184.1.173.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCalcium-Calmodulin-Dependent Protein KinasesCD40 LigandCell Adhesion MoleculesEndothelium, VascularE-SelectinGene ExpressionHumansIntercellular Adhesion Molecule-1Interleukin-1JNK Mitogen-Activated Protein KinasesMembrane GlycoproteinsMitogen-Activated Protein KinasesMolecular Sequence DataOligodeoxyribonucleotidesRNA, MessengerTranscription, GeneticTumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1Intercellular adhesion molecule-1Tumor necrosis factorHuman umbilical vein endothelial cellsAdhesion molecule-1CD40 ligandHomologous desensitizationIL-1E-selectinJun NH2-terminal kinaseNecrosis factorInterleukin-1Molecule-1ATF-2/c-JunEndothelial cellsCell adhesion molecule-1
1995
CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.
Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS. CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4342-4346. PMID: 7538666, PMCID: PMC41940, DOI: 10.1073/pnas.92.10.4342.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, B-LymphocyteCD40 AntigensCell Adhesion MoleculesCells, CulturedCytokinesEndothelium, VascularGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorHumansInterferon-betaInterferon-gammaInterleukinsKineticsMiceRecombinant ProteinsSkinTumor Necrosis Factor-alphaUmbilical VeinsConceptsTumor necrosis factorAdhesion molecule-1Endothelial cellsHuman endothelial cellsNecrosis factorInterleukin-1Molecule-1T cell-mediated inflammatory reactionVascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Cultured human umbilical vein endothelial cellsAdhesion molecule expressionLeukocyte adhesion moleculesMicrovascular endothelial cellsHuman microvascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsFlow cytometric quantitationVein endothelial cellsCD40 antigenEndothelial CD40Interferon betaMolecule expressionInflammatory reactionIL-4 induction of VCAM-1 on endothelial cells involves activation of a protein tyrosine kinase.
Palmer-Crocker RL, Pober JS. IL-4 induction of VCAM-1 on endothelial cells involves activation of a protein tyrosine kinase. The Journal Of Immunology 1995, 154: 2838-45. PMID: 7533184, DOI: 10.4049/jimmunol.154.6.2838.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1VCAM-1 expressionEndothelial cellsIL-4 inductionIL-4IL-4R.Cell adhesion molecule-1Pretreatment of ECTreatment of ECIL-4 concentrationsAdhesion molecule-1Cultured human endothelial cellsHerbimycin ATime of treatmentHuman endothelial cellsTyrosine kinaseMolecule-1Western blotProtein tyrosine kinasesTyrosine phosphorylationIL-4-induced tyrosine phosphorylationAnti-phosphotyrosine immunoprecipitatesReplicate wellsPrdictive Value Of Inducible Endothelial Cell Adhesion Molecule Expression For Acte Rejection Of Human Cardiac Allografts
Briscoe DM, Yeung AC, Schoen FJ, Allred EN, Stavrakis G, Ganz P, Cotran RS, Pober JS, Schoen EL. Prdictive Value Of Inducible Endothelial Cell Adhesion Molecule Expression For Acte Rejection Of Human Cardiac Allografts. Transplantation 1995, 59: 204-211. PMID: 7530872, DOI: 10.1097/00007890-199501000-00009.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAdultBiopsyCD3 ComplexCell Adhesion MoleculesE-SelectinGraft RejectionHeart TransplantationHumansIntercellular Adhesion Molecule-1Longitudinal StudiesMiddle AgedMyocardial IschemiaMyocardiumPredictive Value of TestsProspective StudiesT-LymphocytesVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1Intercellular adhesion molecule-1T-cell infiltratesHuman cardiac allograftsAdhesion molecule expressionVCAM-1 expressionAdhesion molecule-1E-selectinCardiac allograftsCell infiltrateIschemic injuryMolecule expressionEndothelial adhesion molecules intercellular adhesion molecule-1Molecule-1Adhesion molecule intercellular adhesion molecule-1Lesser extent VCAM-1Endothelial cell adhesion molecule expressionEndothelial adhesion molecule expressionAdhesion moleculesCell adhesion molecule-1Ongoing acute rejectionSuccessful antirejection therapyCardiac allograft rejectionHuman transplant recipientsImmediate posttransplant period
1994
Antigen-presenting function of human endothelial cells. Direct activation of resting CD8 T cells.
Epperson DE, Pober JS. Antigen-presenting function of human endothelial cells. Direct activation of resting CD8 T cells. The Journal Of Immunology 1994, 153: 5402-12. PMID: 7989746, DOI: 10.4049/jimmunol.153.12.5402.Peer-Reviewed Original ResearchConceptsCD8 T cellsMemory CD8 T cellsT cell proliferative responsesAllogeneic endothelial cellsCell proliferative responsesT cellsAdhesion molecule-1Endothelial cellsCD8/IL-2Proliferative responseMolecule-1CD4 T cell proliferative responsesLate activation Ag-4Human CD8 T cellsVascular cell adhesion molecule-1Class II MHC expressionIntercellular adhesion molecule-1Cell adhesion molecule-1CD4 T cellsIFN-gamma secretionOrgan graft rejectionIL-2R alpha subunitIntercellular adhesion molecule-2CD8 cellsHuman T-cell-mediated destruction of allogeneic dermal microvessels in a severe combined immunodeficient mouse.
Murray AG, Petzelbauer P, Hughes CC, Costa J, Askenase P, Pober JS. Human T-cell-mediated destruction of allogeneic dermal microvessels in a severe combined immunodeficient mouse. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 9146-9150. PMID: 8090783, PMCID: PMC44764, DOI: 10.1073/pnas.91.19.9146.Peer-Reviewed Original ResearchConceptsSignificant T-cell infiltrationT-cell-mediated injuryT cell-mediated destructionVascular cell adhesion molecule-1Human microvesselsSevere combined immunodeficient (SCID) miceMajor histocompatibility complex (MHC) class II moleculesCell adhesion molecule-1Human allograft rejectionT-cell infiltratesT cell infiltrationCombined Immunodeficient MiceImmunodeficient mouse modelAdhesion molecule-1Human skin graftsClass II moleculesPotential therapeutic reagentSmall animal modelsHuman vascular endotheliumAllograft rejectionSkin rejectionMouse microvesselsSkin graftsT cellsImmunodeficient miceInducibility and Expression of Microvascular Endothelial Adhesion Molecules in Lesional, Perilesional, and Uninvolved Skin of Psoriatic Patients
Petzelbauer P, Pober J, Keh A, Braverman I. Inducibility and Expression of Microvascular Endothelial Adhesion Molecules in Lesional, Perilesional, and Uninvolved Skin of Psoriatic Patients. Journal Of Investigative Dermatology 1994, 103: 300-305. PMID: 7521374, DOI: 10.1111/1523-1747.ep12394720.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Endothelial leukocyte adhesion molecule-1Lesional skinAdhesion molecule-1Psoriatic patientsEndothelial cellsUninvolved skinPerilesional skinMolecule-1Dermal microvasculatureCapillary loopsCell adhesion molecule-1Perivascular inflammatory infiltratesLeukocyte adhesion molecule-1VCAM-1 expressionTumor necrosis factorEndothelial adhesion moleculesMicrovascular endothelial cellsEndothelial cell expressionVenular endothelial cellsInflammatory infiltrateEpidermal involvementPsoriatic plaquesSuperficial plexusNecrosis factor
1993
Elevated cyclic AMP inhibits endothelial cell synthesis and expression of TNF-induced endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1.
Pober JS, Slowik MR, De Luca LG, Ritchie AJ. Elevated cyclic AMP inhibits endothelial cell synthesis and expression of TNF-induced endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1. The Journal Of Immunology 1993, 150: 5114-23. PMID: 7684420, DOI: 10.4049/jimmunol.150.11.5114.Peer-Reviewed Original ResearchConceptsEndothelial cellsAction of cAMPCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsSignal transducerUmbilical vein endothelial cellsRole of cAMPCell adhesion molecule-1Adhesion molecule-1Vein endothelial cellsCyclic monophosphatePhysiological regulatorElevated cyclic AMPVascular cell adhesion molecule-1Adhesion molecule expressionIntracellular mediatorsIsobutyl methylxanthineMolecule-1TNF inductionVCAM-1 mRNAEndothelial activation by hydrogen peroxide. Selective increases of intercellular adhesion molecule-1 and major histocompatibility complex class I.
Bradley JR, Johnson DR, Pober JS. Endothelial activation by hydrogen peroxide. Selective increases of intercellular adhesion molecule-1 and major histocompatibility complex class I. American Journal Of Pathology 1993, 142: 1598-609. PMID: 8098585, PMCID: PMC1886909.Peer-Reviewed Original ResearchConceptsIntercellular adhesion molecule-1Tumor necrosis factorAdhesion molecule-1Major histocompatibility complex class I.Major histocompatibility complex class IHistocompatibility complex class IMolecule-1Complex class IInterleukin-1Intercellular adhesion molecule-1 surface expressionMumol/L hydrogen peroxideVascular cell adhesion molecule-1Transcription factor nuclear factor kappa BEndothelial leukocyte adhesion molecule-1Class ICell adhesion molecule-1Leukocyte-derived cytokinesNuclear factor kappa BVascular endothelial cell functionLeukocyte adhesion molecule-1Surface expressionClass I.Factor kappa BEC surface receptorsEndothelial cell function
1992
Expression of vascular cell adhesion molecule-1 in human renal allografts.
Briscoe DM, Pober JS, Harmon WE, Cotran RS. Expression of vascular cell adhesion molecule-1 in human renal allografts. Journal Of The American Society Of Nephrology 1992, 3: 1180-5. PMID: 1282831, DOI: 10.1681/asn.v351180.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAntigens, CDAntigens, Differentiation, MyelomonocyticCD3 ComplexCell Adhesion MoleculesEndothelium, VascularE-SelectinGraft RejectionHumansIntercellular Adhesion Molecule-1Kidney TransplantationKidney Tubular Necrosis, AcuteKidney TubulesMacrophagesMonocytesPostoperative ComplicationsT-Lymphocyte SubsetsTransplantation, HomologousVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1Cell adhesion molecule-1VCAM-1 expressionAdhesion molecule-1Renal allograftsNormal biopsiesMolecule-1Human renal allograft biopsiesMonocyte/macrophage infiltrateEndothelial VCAM-1 expressionAcute tubular necrosisRenal allograft biopsiesT-cell infiltratesT cell infiltrationHuman renal allograftsEndothelial VCAM-1Normal kidney specimensArea of infiltratesMean scoreVascular endothelial cellsAcute rejectionAllograft biopsiesTubular necrosisCell infiltrateClinicopathologic diagnosisEffects of tumor necrosis factor, lipopolysaccharide, and IL-4 on the expression of vascular cell adhesion molecule-1 in vivo. Correlation with CD3+ T cell infiltration.
Briscoe DM, Cotran RS, Pober JS. Effects of tumor necrosis factor, lipopolysaccharide, and IL-4 on the expression of vascular cell adhesion molecule-1 in vivo. Correlation with CD3+ T cell infiltration. The Journal Of Immunology 1992, 149: 2954-60. PMID: 1383333, DOI: 10.4049/jimmunol.149.9.2954.Peer-Reviewed Original ResearchConceptsT cell infiltrationEndothelial VCAM-1 expressionVCAM-1 expressionIL-4Cell infiltrationEndothelial leukocyte adhesion moleculeVCAM-1T cellsAbility of TNFT-cell-rich infiltrateImmunocytochemical stainingVascular cell adhesion molecule-1Endothelial cell adhesion molecule expressionAdhesion moleculesCell adhesion molecule-1Adhesion molecules ELAM-1Cell adhesion molecule expressionInjection of LPST cell extravasationAdhesion molecule expressionEndothelial VCAM-1Tumor necrosis factorAdhesion molecule-1Endothelial adhesion moleculesLeukocyte adhesion molecules
1991
Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells
Savage C, Hughes C, Pepinsky R, Wallner B, Freedman A, Pober J. Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells. Cellular Immunology 1991, 137: 150-163. PMID: 1679377, DOI: 10.1016/0008-8749(91)90065-j.Peer-Reviewed Original ResearchConceptsLymphocyte function-associated antigen-3Adhesion molecule-1T cellsEndothelial cellsMolecule-1Antigen 3Human peripheral blood CD4Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Peripheral blood CD4T cell populationsB7/BB1Cultured human endothelial cellsIL-2 synthesisBlood CD4EC costimulationHuman endothelial cellsT cell CD2IL-2CD4CostimulationCell populationsComparable degreeLines of evidence